Glutathione S-transferase Omega class (GSTO) polymorphisms in a sample from Rome (Central Italy)

Abstract

Glutathione S-transferases are a superfamily of enzymes that are involved in biotransformation of drugs, xenobiotics and play a fundamental role in the protection of cells from oxidative stress. In humans, the recently described GST Omega class contains two expressed genes GSTO1 and GSTO2, located on chromosome 10 (10q24.3). Four polymorphisms in GSTO genes have been identified in ethnic groups: GSTO1*A140D (rs4925), GSTO1*E155del (rs56204475), GSTO1*E208K (rs11509438) and GSTO2*N142D (rs156697). This study provides the allele frequencies of GSTO polymorphism in a sample consisting of 116 apparently healthy individuals of both sexes from Rome (Central Italy). Detection of GSTO1*A140D and GSTO2*N142D alleles was performed by PCR-RFLP analysis, while GSTO1*E155del and GSTO1*E208K alleles were detected using the Confronting Two-Pair Primers analysis (PCR-CTPP) and allele specific PCR, respectively. The GSTO allele frequencies found in the Italian sample were included in the variability range observed in European populations. Comparison between the data presented in this study and data in previous studies showed different patterns among European, Asian and African populations.

GSTM1 null genotype as risk factor for late-onset Alzheimer's disease in Italian patients

“…Glutathione S-transferases (GSTs; EC: 2.5.1.18) comprise a superfamily of genes encoding enzymes that are very important in the clinical outcome of different multifactorial diseases [14][15][16]. These enzymes are involved in metabolic detoxification of reactive electrophiles, in biosynthesis of leukotrienes, prostaglandins, testosterone, and progesterone, and in the degradation of tyrosine [17,18]. Over the last two decades, a significant body of data has been accumulated linking genetic variability of GSTs with the development and expression of several multifactorial diseases [19][20][21].…”

Association Between Glutathione S-Transferase Omega 1 A140D Polymorphism in the Turkish Population and Susceptibility to Non-Small Cell Lung Cancer

“…The frequency of GSTO1 A140D seems to vary between races (9,(11)(12)(13)(14)(15)(16)(17)(18), but only a few studies investigated this frequency in Caucasians (9,14,17,18). In the single study of GSTO1 A140D that included the Turkish population, Takeshita et al (17) observed that its distribution differed from Caucasian populations, and was more similar to African, Eastern Asian, and Brazilian populations.…”

Haplotype differences for copy number variants in the 22q11.23 region among human populations: a pigmentation-based model for selective pressure

“…Information about the classification of these human groups and DNA extraction procedures is available through previous studies. [18][19][20][21] We exported additional data from the databases of HapMap and 1000 Genomes Projects. 22 …”