Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies

Abstract

Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, it is difficult to reach the cell dose required for cell therapy of vascular diseases, thus limiting the clinical use of these cells. Glycosaminoglycans (GAG) are components from the extracellular matrix (ECM) that are able to interact and potentiate heparin binding growth factor (HBGF) activities. According to these relevant biological properties of GAG, we designed a GAG mimetic having the capacity to increase the yield of ECFC production from blood and to improve functionality of their endothelial outgrowth. We demonstrate that the addition of [OTR(4131)] mimetic during the isolation process of ECFC from Cord Blood induces a 3 fold increase in the number of colonies. Moreover, addition of [OTR(4131)] to cell culture media improves adhesion, proliferation, migration and self-renewal of ECFC. We provide evidence showing that GAG mimetics may have great interest for cell therapy applied to vascular regeneration therapy and represent an alternative to exogenous growth factor treatments to optimize potential therapeutic properties of ECFC.

Human and Swine Dental Pulp Stem Cells Form a Vascularlike Network after Angiogenic Differentiation in Comparison with Endothelial Cells: A Quantitative Analysis

“…Next we applied a quantitative method (14) to analyze the in vitro angiogenesis assays of these cells. The quantitative analysis was based on the images taken at 4 h presented in Fig.…”

“…The images were captured at ×4 in phase contrast mode and the vascular network was analyzed using ImageJ software according to a method reported by Chevalier et al (14). Briefly, the vascular networks were segmented and skeletonized.…”

Oncogenic role of sFRP2 in P53-mutant osteosarcoma development via autocrine and paracrine mechanism

“…Images were analyzed with Image J Angiogenesis Analyzer tool. This tool quantifies the tube formation images by extracting characteristic information of the formed tube meshes and branches (Chevalier et al, 2014).…”

Fabrication and Characterization of Heparin/Collagen Sponge for in Vitro Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Hepatocytes

“…Inpress(Inpress):e40599. 7 the endothelial outgrowth by interacting with heparinbinding growth factors (38). This provides a great opportunity for heparinized/collagen-3D sponges to be served as a good vehicle for cell therapy as well as for promoting angiogenesis.…”