Glycosphingolipids of an alveolar rhabdomyosarcoma and normal human muscle

Leskawa, Kenneth C.; Buse, Paul E.; Hogan, Edward L.; Garvin, A. Julian

doi:10.1007/bf02834169

Cited by 8 publications

(2 citation statements)

References 22 publications

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“…Following neutralization with HC1, samples were dialyzed for several days at 4 C and dried in an evaporating centrifuge (Savant Instruments). With some samples (the influenza vaccines produced by Wyeth and the rubella vaccine from Merck, Sharp and Dohme [West Point, Pennsylvania]), it was necessary to remove excessive neutral lipids by chromatography on small columns of silicic acid (29). Final dried residues were dissolved in 100 td of C/M, 2:1 (v/v), and 40 ~1 aliquots were applied to silica gel TLC plates.…”

Section: Methodsmentioning

confidence: 99%

Lipid content of swine influenza and other vaccines

Leskawa

Hogan

Dasgupta

et al. 1986

Lipids

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An analysis of the lipids in swine influenza vaccines was performed, comparing six different lots of swine influenza, other influenza and noninfluenza vaccines. Cholesterol content and phospholipid content varied greatly, but there were no major differences between the types of vaccines. Appreciable amounts of phosphatidylethanolamine were found in only one swine influenza vaccine. The major phospholipids of influenza vaccines were phosphatidylcholine, sphingomyelin and phosphatidic acid. A detectable amount of phosphatidylserine was not found in any swine influenza vaccine, but was present in two of three nonswine influenza vaccines. Only two of six swine influenza vaccines showed trace amounts (less than 0.5 microgram/ml) of ganglioside (GM3). However, larger quantities of galactocerebroside were found (2.24-6.43 micrograms/ml) in all influenza vaccines examined, including swine influenza vaccines.

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Section: Methodsmentioning

confidence: 99%

Lipid content of swine influenza and other vaccines

Leskawa

Hogan

Dasgupta

et al. 1986

Lipids

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“…The extracts were dried under N2, saponified in 0.6 N NaOH in methanol and desalted by C18 reverse-phase chromatography [34]. Removal of fatty acid methyl esters and other neutral lipids was performed by silicic acid column chromatography [35]. and fu-1 cells were collected on varying days, fixed with methanol and stained with Giemsa stain.…”

Section: Gsl Isolationmentioning

confidence: 99%

Glycosphingolipids during skeletal muscle cell differentiation: Comparison of normal and fusion-defective myoblasts

Cambron

Leskawa

1994

Mol Cell Biochem

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The regulation of glycosphingolipid (GSL) synthesis in culture by fusion-competent (E63) myoblasts and fusion-defective (fu-1) cells was examined. Upon reaching confluency E63 cells fused to form multinucleated myotubes and demonstrated many characteristics of developing skeletal muscle including induction of creatine kinase activity and a shift in creatine kinase isozymes to the MM isoform. The fu-1 cells displayed none of these characteristics, despite the fact that both cells were cloned from the same parental myoblast line (rat L8). There was a transient increase in the synthesis of total neutral GSLs by E63 cells at the time of membrane fusion. In contrast, neutral GSL synthesis by fu-1 cells gradually decreased with time in culture. The major GSLs synthesized by both cell types were lactosylceramide and ganglioside GM3, with more complex structures being observed with prolonged time in culture. Several glycosyltransferase activities were assayed at varying times in culture. Generally, the changes in activities fell into three groups. One group was maximally activated at the end of the culture period (GalT-3, GalNAcT-1 and GalT-6). Another group was maximally activated during the time of active membrane fusion (GlcT and SAT-1). A third group was maximally activated at the time of cell contact and the beginning of membrane fusion (GlcNAcT-1 and GalT-2). In terms of the times of maximal activation there were few differences between E63 and fu-1 cells, with one notable exception. The activity of GalT-2 (lactosylceramide synthase) in E63 cells increased dramatically upon contact and the beginning of membrane fusion, whereas there were no changes in GalT-2 activity in fu-1 cells during time in culture. These results support our hypothesis that membrane glycosphingolipids play an important role in the differentiation of skeletal muscle cells.

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Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro

et al. 1988

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Glycosphingolipid biosynthesis was examined using [3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. Incorporation of label into neutral glycolipids decreased steadily as the population of myotubes increased, so that final biosynthesis was one-half that observed with myoblasts (p less than 0.02). Conversely, ganglioside biosynthesis doubled during myoblast confluency (p less than 0.02) and then decreased as myotubes formed. Qualitatively, L6 cells synthesized large amounts of ganglioside GM3 during all myogenic phases. The major neutral glycosphingolipid products were lactosylceramide and paragloboside (nLcOse4Cer). Few changes in TLC autoradiographic patterns were noted during differentiation, with the exception of a slight decrease in ganglioside GM1. The results indicate that the biosynthesis of glycosphingolipids is tightly regulated during myogenesis in vitro and suggest a role for membrane gangliosides in muscle cell differentiation.

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Glycosphingolipids of an alveolar rhabdomyosarcoma and normal human muscle

Cited by 8 publications

References 22 publications

Lipid content of swine influenza and other vaccines

Lipid content of swine influenza and other vaccines

Glycosphingolipids during skeletal muscle cell differentiation: Comparison of normal and fusion-defective myoblasts

Glycosphingolipid biosynthesis during myogenesis of rat L6 cells in vitro

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