GmAOC4 modulates seed germination by regulating JA biosynthesis in soybean

Zhang, Wei; Xu, Wenjing; Li, Songsong; Zhang, Hongmei; Liu, Xiaoqing; Cui, Xiaoyan; Song, Li; Zhu, Yuelin; Chen, Huatao

doi:10.1007/s00122-021-03974-0

Cited by 10 publications

(10 citation statements)

References 30 publications

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“…Levels of these transcripts are increased by 80 to 100-fold in turions as compared to fronds. In addition to ABA biosynthesis, a gene encoding allene oxide cyclase, the key enzyme for JA biosynthesis (Zhang et al ., 2002), is also increased by about 50-fold in turions. This indicates that JA responsive pathways may also play important roles in turion biology and is reminiscent of its involvement along with ABA in the regulation of seed germination (Zhang et al ., 2002).…”

Section: Resultsmentioning

confidence: 99%

“…In addition to ABA biosynthesis, a gene encoding allene oxide cyclase, the key enzyme for JA biosynthesis (Zhang et al ., 2002), is also increased by about 50-fold in turions. This indicates that JA responsive pathways may also play important roles in turion biology and is reminiscent of its involvement along with ABA in the regulation of seed germination (Zhang et al ., 2002). Other classes of enzymes that showed significant increases in transcript levels are those involved in DNA and protein damage repair, as well as enzymes for cell wall modifications that could facilitate rapid growth during turion germination (Tables S3 to S5).…”

Section: Resultsmentioning

confidence: 99%

“…Consistent with their increased expression, we found some of their target genes encoding HSP20 and HSP70, as well as a cold-regulated 413 (Cor413) membrane protein that could confer temperature stress protection (Zhou et al, 2018), being highly expressed in turions. Additional protection from DNA damage and endoplasmic reticulum dysfunction could be provided by up-regulation in turions of poly (ADP-ribose) polymerase (Aoyagi et al, 2021) and the conserved eukaryotic cell death repressor Bax Inhibitor-1 (BI-1) seed germination (Zhang et al, 2002). Other classes of enzymes that showed significant increases in transcript levels are those involved in DNA and protein damage repair, as well as enzymes for cell wall modifications that could facilitate rapid growth during turion germination (Tables S3 to S5).…”

Section: Comparative Analysis Of Global Transcriptomes Between Mature...mentioning

confidence: 99%

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Genomics of turions from the Greater Duckweed reveal its pathways for dormancy and reemergence strategy

Pasaribu

Acosta

Aylward

et al. 2022

Preprint

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Over 15 families of aquatic plants are known to use a strategy of developmental switching upon environmental stress to produce dormant propagules called turions. However, few molecular details for turion biology have been elucidated due to the difficulties in isolating high-quality nucleic acids from this tissue. We successfully developed a new protocol to isolate high-quality transcripts and carried out RNA-seq analysis of mature turions from the Greater Duckweed Spirodela polyrhiza. Comparison of turion transcriptome to that of fronds, the actively growing leaf-like tissue, were carried out. Bioinformatic analysis of high confidence, differentially expressed transcripts between frond and mature turion tissues revealed major pathways related to stress tolerance, starch and lipid metabolism, and dormancy that are mobilized to reprogram frond meristems for turion differentiation. We identified the key genes that are likely to drive starch and lipid accumulation during turion formation, as well as in pathways for starch and lipid utilization upon turion germination. Comparison of genome-wide cytosine methylation levels also revealed evidence for epigenetic changes in the formation of turion tissues. Similarities between turions and seeds provided evidence that key regulators for seed maturation and germination have been retooled for their function in turion biology.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Comparative Analysis Of Global Transcriptomes Between Mature...mentioning

confidence: 99%

See 1 more Smart Citation

Genomics of turions from the Greater Duckweed reveal its pathways for dormancy and reemergence strategy

Pasaribu

Acosta

Aylward

et al. 2022

Preprint

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“…Современные методики белковой инженерии позволяют не проводить долгий мониторинг возможных нуклеотидных полиморфизмов и отбор нужных вариантов, после чего осуществляется гибридизация с целью закрепления полезных SNPs [7,8]. С использованием биоинформатического анализа перед исследователями в области биотехнологии открываются возможности искусственной индукции подобных полиморфизмов с заранее заданными и известными свойствами.…”

Section: Introductionunclassified

Bioinformatic method for determining single nucleotide polymorphisms on the example of gene <i>WIN</i> in <i>Glycine max</i>

Timkin

Penzin

2023

Izvestiâ vuzov. Prikladnaâ himiâ i biotehnologiâ

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In this paper, a hypothetical method for locating SNPs (single nucleotide polymorphisms) on the example of the ribonuclease gene WIN was proposed. Ribonuclease comprises an enzyme that participates in defence reactions against fungal infections in soybeans, as well as other protective responses to biotic stress. Its belonging to the RNA-ases group determines the specific properties, namely the ability to degrade foreign nucleic acids. This ability provides for a general nonspecific immune response of the plant to the invasion of antigenic structures. Modern biotechnology calls for the development of molecular methods and approaches that will increase the resistance of a culture or accelerate the processes of its adaptation in the field. This problem can be solved by using technologies of SNP artificial induction in those parts of the genome that encode proteins capable of acting in protective reactions against biotic stress. In the study, 5 single-nucleotide polymorphisms were proposed using bioinformatic analysis. Since the localisation and detection of SNPs comprise a challenging task due to the presence of a single nucleotide change, in the biotechnological practice, predictive analysis is carried out in order to localise the potential sequence of occurring single-nucleotide polymorphism. Following the identification of the hypothetical SNP location, they can be further detected using complex molecular methods, such as real-time PCR or local sequencing. This technology can become a powerful tool for breeding soybean varieties having predetermined properties. Such theoretical and predictive models will allow for a quicker response to the dynamic environment under manmade load on plants.

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“…In association panels, characteristics such as LD, genetic diversity, marker density, and population structure affect the resolution and accuracy of QTL detected via GWAS (Sonah et al, 2015;Zhang et al, 2022). In recent years, GWAS have not only been applied to different populations to identify QTL associated with PC in soybean (Sonah et al, 2015;Zhang et al, 2017;Li et al, 2019;Zhang T et al, 2019), but have also been applied to analyze other complex quantitative traits such as oil content, salt stress tolerance, agronomic traits, and yieldrelated traits (Hwang et al, 2014;Fang et al, 2017;Zhang W et al, 2019;Pang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Identification of loci governing soybean seed protein content via genome-wide association study and selective signature analyses

Zhang

et al. 2022

Front. Plant Sci.

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Soybean [Glycine max (L.) Merr.] is an excellent source of protein. Understanding the genetic basis of protein content (PC) will accelerate breeding efforts to increase soybean quality. In the present study, a genome-wide association study (GWAS) was applied to detect quantitative trait loci (QTL) for PC in soybean using 264 re-sequenced soybean accessions and a high-quality single nucleotide polymorphism (SNP) map. Eleven QTL were identified as associated with PC. The QTL qPC-14 was detected by GWAS in both environments and was shown to have undergone strong selection during soybean improvement. Fifteen candidate genes were identified in qPC-14, and three candidate genes showed differential expression between a high-PC and a low-PC variety during the seed development stage. The QTL identified here will be of significant use in molecular breeding efforts, and the candidate genes will play essential roles in exploring the mechanisms of protein biosynthesis.

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GmAOC4 modulates seed germination by regulating JA biosynthesis in soybean

Cited by 10 publications

References 30 publications

Genomics of turions from the Greater Duckweed reveal its pathways for dormancy and reemergence strategy

Genomics of turions from the Greater Duckweed reveal its pathways for dormancy and reemergence strategy

Bioinformatic method for determining single nucleotide polymorphisms on the example of gene <i>WIN</i> in <i>Glycine max</i>

Identification of loci governing soybean seed protein content via genome-wide association study and selective signature analyses

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