GMOMETHODS: The European Union Database of Reference Methods for GMO Analysis

Bassolino, Laura; Bulcke, Marc H Van den; Mazzara, Marco; Ben, Enrico; Patak, Alex

doi:10.5740/jaoacint.12-050

Cited by 53 publications

(42 citation statements)

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“…W tym celu stosowano metody specyficzne dla poszczególnych linii GMO, zwalidowane i uznane za zalecane do badań GMO w całej Unii Europejskiej. Do identyfikacji linii GMO i oznaczenia jej zawartości stosowano metody wykorzystujące technikę real-time PCR (1,4). (10,11).…”

Section: Materiał I Metodyunclassified

Application of molecular biology in the studies towards genetically modified organisms used in feed in Poland

Sieradzki

Mazur

Król

et al. 2017

Medycyna Weterynaryjna

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Genetically modified organisms are increasingly used in the production of feed and food, which has met with opposition from consumers. The aim of the study was the use of molecular biology methods with particular emphasis on techniques of real-time PCR in the research in the detection and identification of genetically modified feed. The research materials were samples of feed taken from feed produced and used in animal nutrition in Poland in the years 2004–2015. The applied research methods included PCR and real-time PCR techniques, and consisted in the detection and determination of the DNA content of genetically modified plants. Cascade methods used in this study included the screening method of detection of GMOs, the method of identifying the type of GMO, and methods of quantitative analysis of GMO content. As part of the research task in the years 2004–2015 a total of 1435 samples of feed towards GMOs were examined. A positive result was found in 559 cases (39%). Most frequently the positive samples were found the presence of genetically modified soybeans (531, 37%). Moreover, within the years 2014–2015 an increase in the number of positive GM rape samples was observed (56, 4%). GM maize contained the least positive samples (38, 2.6%). GMO content above the legislative threshold 0.9% was found in the vast majority of samples containing GM soy, while for maize and rapeseed the number of samples containing more than 0.9% GMO was respectively 12 and 8. Analysis of the feed market in Poland indicates that the soybean plant is the most common genetically modified crop. Analysis of the origin of sources of GM rapeseed showed that the reasons for this should be sought in batches of rapeseed imported from third countries. It has been observed with regard to the situation of GM maize for the feed market in Poland that from 2013 the situation changed radically as a result of the Decree of the Ministry of Agriculture issued prohibiting the cultivation of MON810 maize on Polish fields. The result of our study showed that the proportion of genetically modified feed on the feed market in Poland is very similar to other EU countries. The source of GMOs in feed on the Polish market is feed materials imported into Poland as a source of feed protein.

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Section: Materiał I Metodyunclassified

Application of molecular biology in the studies towards genetically modified organisms used in feed in Poland

Sieradzki

Mazur

Król

et al. 2017

Medycyna Weterynaryjna

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“…or '-' symbols. GM events not detected by any screening method or not detected by the original screening set of Waiblinger et al (2010) are indicated by grey shading a An authorization decision is taken or is pending b QT-ELE-00-004 (Bonfini et al 2012) c QL-ELE-00-011 (Bonfini et al 2012) d QL-ELE-00-014 (Bonfini et al 2012) e QL-CON-00-008 (Bonfini et al 2012) f QL-CON-00-011 (Bonfini et al 2012) g QT-ELE-00-002 (Bonfini et al 2012) h QL-ELE-00-016 (Bonfini et al 2012) i QL-ELE-00-015 (Bonfini et al 2012) J Not detected by any screening method a FAM and HEX signal) can be analysed in one reaction. Furthermore, in case of a positive finding for a sample DNA, the subsequent analysis for identification is less complex if only a FAM or HEX signal is detected.…”

Section: Assay Design and Optimisationmentioning

confidence: 99%

“…At the time we began the study, six GMO soybean events (MON 87701,MON 87708,MON 87769, were identified as not being covered by the Waiblinger screening table. Protocols for six single event-specific real-time PCR methods using fluorescein amidite (FAM) labeled TaqMan probes and certified reference materials are publicly available (Bonfini et al 2012). If the six methods combine in one PCR reaction, a positive FAM fluorescence signal would indicate a positive screening result for at least one of the GM soybean events, without knowing specifically which of the GM event(s) actually is present in the sample.…”

Section: Introductionmentioning

confidence: 99%

“…A direct and practicable way to detect GM events is to apply the single event-specific methods provided by the European Union Reference Laboratory for GM Food and Feed (EURL-GMFF) according to Regulation (EC) No. 1829/2003, if available (EU 2003Bonfini et al 2012). Detection of any GM soybean event in seed lots becomes particularly more important since European initiatives have launched programmes to increase non-GM soybean protein production, based on largescale soybean cultivation in the EU (De Visser et al 2014;Anonymous 2016).…”

Section: Introductionmentioning

confidence: 99%

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Screening for six genetically modified soybean lines by an event-specific multiplex PCR method: Collaborative trial validation of a novel approach for GMO detection

Grohmann

Belter

Speck

et al. 2016

J Consum Prot Food Saf

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This study presents a novel approach to detect genetically modified (GM) plant events that are not covered by common GMO screening methods. It is based on a simplified multiplex assay which merges the event-specific real-time PCR methods for the detection of six GM soybean lines (MON 87701, MON 87708, MON 87769, DP-305423, CV-127 and DAS-68416). The use of two different fluorescent dyes facilitates the subsequent analysis for identification of the GM event. The multiplex PCR method was validated in a collaborative study trial with 16 participating laboratories. Each laboratory received eight samples containing low levels (0.1% or 0.03% m/m) of one or two GM soybean lines and four GMnegative samples. Data of 720 PCR analyses were evaluated and a false-positive rate of 0.3% and a falsenegative rate of 3.9% was observed, respectively. The limits of detection (LOD 95%) were calculated based on modelling the probability of detection (POD) and show satisfactory sensitivity and reproducibility for the assay. Furthermore, we discuss the modularity and applicability of event-specific multiplex PCR systems for the detection of GM events that are not covered by screenings.

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“…Thus the authors claim, confirming the transgenic Biodivers Conserv (2018) 27:539-566 543 Table 1 Evidence table of sampling In searching for p35-S and T-NOS, Quist and Chapela were applying a screening method based on primers that target common elements of many commercial transgenic constructs. This is still the most widely applied screening matrix worldwide (Bonfini et al 2012;Block et al 2013). The method of nested-PCR was used to confirm non-specific binding and the amplification of unexpected primer binding sites.…”

Section: Transgenes In Mexican Maizementioning

confidence: 99%

Challenges for transgene detection in landraces and wild relatives: learning from 15 years of debate over GM maize in Mexico

Agapito-Tenfen

Wickson

2017

Biodivers Conserv

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Maize is one of the world's five staple cereals and its traditional varieties constitute a global resource critical to future agricultural development. Fifteen years ago, claims that transgenes had spread into traditional landrace maize in Mexico started an international discussion on the scale and significance of transgene flow from genetically modified (GM) crops to centres of crop origin and genetic diversity. The initial discovery of transgenes in landrace maize sparked an intense environmental dispute in which the culture and traditions of indigenous people were seen as threatened by the unchecked spread of biotechnological inventions from multinational corporations. This dispute was reflected in a political and legal battle over the regulatory status of GM crops in Mexico, which continues today as approvals of GM maize for cultivation remain subject to contestation in the courts. These legal, political and environmental disputes have been fanned by the existence of a significant scientific controversy over the methods for GM detection. The use of various approaches and a lack of harmonized methods specific for monitoring and detection of transgenes in landraces has generated both positive and negative results for GM contamination in Mexico over the years. In this paper, we review the peerreviewed literature on transgene detection in Mexican maize and highlight the challenges associated with transgene detection in landraces. In doing so, we identify the key methodological aspects under dispute and pinpoint the research bottlenecks and needs for building the capacity to effectively monitor transgene escape from GM crops to wild relatives or landraces.

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GMOMETHODS: The European Union Database of Reference Methods for GMO Analysis

Cited by 53 publications

References 0 publications

Application of molecular biology in the studies towards genetically modified organisms used in feed in Poland

Application of molecular biology in the studies towards genetically modified organisms used in feed in Poland

Screening for six genetically modified soybean lines by an event-specific multiplex PCR method: Collaborative trial validation of a novel approach for GMO detection

Challenges for transgene detection in landraces and wild relatives: learning from 15 years of debate over GM maize in Mexico

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