Gnathostoma binucleatum: Excretion–secretion antigen analysis obtained from advanced third-stage larvae in in vitro culture

Caballero-García, María de Lourdes; Almeyda-Artigas, Roberto Javier; Mosqueda-Cabrera, Miguel Ángel; Jiménez-Cardoso, Enedina

doi:10.1016/j.exppara.2005.03.012

Cited by 10 publications

(12 citation statements)

References 21 publications

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“…26 The SDS-PAGE patterns for AgES, with 22 bands 6-190 kDa ( Figure 1C), were more complex and different from those in L3A recovered from experimentally infected mice (28-208 kDa). 16 Two-dimensional immunoblot analysis patterns from this study ( Figure 1D) were even more complex, showing 59 spots (9-75 kDa, pI 6-7.5).…”

Section: Discussionmentioning

confidence: 99%

“…Our results contrasted with those reported for AgES of L3 obtained from infected mice in that three bands (80, 120, and 208 kDa) were found by using serum samples from infected mice, and one reactive band (120 kDa) was detected with a human serum sample from an infected person. 16 We report IET patterns and two-dimensional immunoblot analysis of AgS ( Figure 4) and AgES ( Figure 5) obtained from L3A of G. binucleatum. Four peptides in AgS, three with molecular weights of 32 kDa (pI 6.3, 6.5 and 6.9) and one with a molecular weight of 40 kDa (pI 5.6), were identified as specific antigens (Figure 4) that showed no crossreactivity with serum samples from healthy persons.…”

Section: Discussionmentioning

confidence: 99%

“…Two of the peptides were metalloproteases (80 and 208 kDa). 16,17 The purpose of this study was to identify and characterize G. binucleatum immunodominant antigens derived from somatic antigens (AgS) and excretory-secretory antigens (AgES) that could be useful for immunodiagnosis of human gnathostomiasis cases.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Immunodominant Peptides from Gnathostoma binucleatum

Campista-León

Delgado‐Vargas²,

Landa³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of Gnathostoma binucleatum and in Asia mainly by G. spinigerum. The availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. In this study, six immunodominant peptides were identified and characterized from G. binucleatum, somatic antigens (AgS: 24, 32, and 40 kDa) and excretory-secretory antigens (AgES: 42, 44, and 56 kDa) by two-dimensional immunoblot analysis. Among those immunodominant peptides, two AgS spots were characterized by mass spectrometric analysis (32 kDa; pI 6.3 and 6.5) and identified as type 1 galectins. In accordance with this finding, a fraction of AgS exhibited affinity to lactose and displayed a 100% specificity and sensitivity for the diagnosis of human gnathostomiasis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification of Immunodominant Peptides from Gnathostoma binucleatum

Campista-León

Delgado‐Vargas²,

Landa³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…It is a food-borne zoonosis, and is endemic to areas where humans eat raw freshwater fish or shellfish, especially Thailand and other parts of Southeast Asia, Japan, and increasingly Latin America, particularly Mexico. 1 It is acquired by consuming raw or uncooked fresh-water fish infected with ADVL3, 2 in particular, swamp eels, catfish, sleeper perch, bream, Nile tilapia, butterfish, loaches, or snake-headed fish. 1 Epidemiologic studies have shown that more than 2,000 cases of this disease have been reported in Mexico since 1999, of which more than 500 were reported in the state of Nayarit.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano‐Zaragoza¹,

Durán‐Avelar²,

Messina-Robles³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked freshwater fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting. Our results do not show differences in the antigens recognized by IgM and IgE. However, we found that the specific humoral immune response is caused mainly by IgG, specifically IgG4. We found that 43%, 65.2%, 54.1%, and 26.3% of the patients recognize the 37-kD, 33-kD, 31-kD, and 24-kDa antigens, suggesting that the 33-kD antigen is the immunodominant antigen of G. binucleatum.

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“…For the genus Gnathostoma, only a few proteases have been characterized, but they include the 24 kDa, cathepsin L-like cysteine protease from Gnathostoma spinigerum (Kongkerd et al, 2008), which has been proposed for use as a specific antigen in diagnostics. In the case of G. binucleatum, Caballero et al (2005) reported two bands with proteolytic activity against gelatin at 205 and 80 kDa. They also found that the 80 kDa protease activity was inhibited by EDTA, which allowed them to affirm that it was a metalloprotease.…”

Section: Resultsmentioning

confidence: 99%

Proteases secreted by Gnathostoma binucleatum degrade fibronectin and antibodies from mammals

Vibanco-Pérez¹,

Durán‐Avelar²,

Zambrano‐Zaragoza³

et al. 2015

Helminthologia

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SummaryGnathostomiasis is a prevalent zoonosis in humans in some regions of the world. The genus Gnathostoma is considered an accidental parasite for humans; G. binucleatum is the endemic species in Nayarit, Mexico. This work was designed to determine the proteolytic activity of the excretory-secretory products (ESP) of advanced third-stage larvae (ADVL3) of Gnathostoma binucleatum against human fibronectin and antibodies from human and sheep. Our findings showed protease activity against human fibronectin as well as sheep and human gamma globulins of the ESP at molecular weights of 80 and 56 kDa. The proteases found in the ESP of G. binucleatum are thus candidate molecules for consideration as pathogenic elements, owing to the fact that they destroy proteins of the host tissue, which probably allows them to migrate through those tissues and degrade molecules involved in the humoral immune response.

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Gnathostoma binucleatum: Excretion–secretion antigen analysis obtained from advanced third-stage larvae in in vitro culture

Cited by 10 publications

References 21 publications

Identification of Immunodominant Peptides from Gnathostoma binucleatum

Identification of Immunodominant Peptides from Gnathostoma binucleatum

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Proteases secreted by Gnathostoma binucleatum degrade fibronectin and antibodies from mammals

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