Gokilaht®-S 5EC testing on Culex quinquefasciatus Say larvae for an early detection in esterase and monooxygenase resistance system

Singh, Gavendra; Prakash, Soam

doi:10.1007/s00436-008-1292-x

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…aegypti (Lin et al, 2013;Koou et al, 2014b;Rasli et al, 2018) and Cx. quinquefasciatus Say (Diptera: Culicidae) (Sarkar et al, 2009;Singh & Prakash, 2009;Low et al, 2013b;Ramkumar & Shivakumar, 2015). Their results were inconsistent with the present study, which did not reveal any correlation related to the survivability rate of all insecticides analyzed against α-EST activity.…”

Section: Discussioncontrasting

confidence: 99%

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

et al. 2021

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This study aims to examine the efficacy of mosquito mat vaporizers on Aedes aegypti and their associated metabolic detoxication mechanisms. For this purpose, Aedes aegypti (Linnaeus) was collected from nine districts in Selangor, Malaysia and tested with mosquito vaporizing mat bioassays. The same populations were also subjected to biochemical assays to investigate activities of detoxifying enzymes, namely non-specific esterase (EST), glutathione-S-transferase (GST) and mixed function oxidase (MFO). The efficacy of Ae. aegypti on the active ingredients tested in decreasing order were d-allethrin > dimefluthrin > prallethrin with PBO > prallethrin. The results further indicated significant enhancement mean levels of EST, GST and MFO in pyrethroid-resistant populations. The mortality rate of Ae. aegypti in response to pyrethroid active ingredients was associated with MFO activity, suggesting it is an important detoxification enzyme for the populations tested. In view of the presence of resistance against household insecticide products, pyrethroid efficacy on Ae. aegypti populations needs to be monitored closely to ensure the implementation of an effective vector control program in Malaysia.

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Section: Discussioncontrasting

confidence: 99%

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

et al. 2021

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Evaluation of culture filtrates of Culicinomyces clavisporus: Mycoadulticide for Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi

Singh

Prakash

2011

Parasitol Res

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The Culicinomyces clavisporus is a fungal pathogen of a wide range of mosquito larvae. The C. clavisporus was isolated from the larvae of Culiseta inornata. We have investigated into potential pathogenicity against the adults of Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi. The culture filtrates released from the strain of C. clavisporus 46258 were grown in the EmYPss broth, were filtered and used for the bioassays after a growth of 15 days. The results demonstrated these metabolites with LC(50), LC(90) and LC(99) values of C. quinquefasciatus, 5.62, 8.71 and 12.59, A. aegypti, 3.0, 7.0 and 9.3, and A. stephensi 2.69, 6.0 and 7.24 μl/cm(2), respectively after exposure for 24 h. These results compared favorably with the commercial adulticide Gokilaht(®)-S 5EC (d,d-trans-cyphenothrin) that showed 100% mortality at the same concentration. This study successfully identified that the metabolites of C. clavisporus can be used as mosquitoes adulticide as safer alternative to modern synthetic chemical insecticide against mosquito vector of diseases. Further purification can lead to biotechnological exploitation.

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Efficacy of the Trichophyton ajelloi and Lagenidium giganteum metabolites against mosquitoes after flash chromatography

Singh

Prakash

2011

Parasitol Res

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Entomopathogens are significant natural enemies for mosquitoes. We have investigated the adulticidal efficacies of metabolites of Trichophyton ajelloi and Lagenidium giganteum against Culex quinquefasciatus and Aedes aegypti simultaneously. The T. ajelloi was grown on Sabouraud's dextrose broth medium at 25 ± 2°C and relative humidity at 75 ± 5% for 15 days. L. giganteum was grown in peptone yeast extract glucose broth at 25 ± 2°C and relative humidity 75 ± 5% for 15 days. The filtrations of metabolites have been made by using Whatman-1 filter paper then with the flash chromatograph. The bioassays were conducted as per the World Health Organization's methods and protocols (2006). In this significant investigations, the metabolites of T. ajelloi have been found highly susceptible against A. aegypti with LC(99)-7.24 ml after an exposure time of 24 h with a comparison, the LC(99)-66 ml was observed against C. quinquefasciatus after exposure of 24 h. Moreover, the L. giganteum metabolites have shown higher pathogenicity against C. quinquefasciatus with LC(99)-11.3 ml and A. aegypti with LC(99)-15.49 ml. Although, the efficacy in adults could be achieved with higher concentration can be significant also. Their adulticidal activities in different climatic zones are plausible with metabolites which have better LT values of T. ajelloi.

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Gokilaht®-S 5EC testing on Culex quinquefasciatus Say larvae for an early detection in esterase and monooxygenase resistance system

Cited by 4 publications

References 15 publications

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

Evaluation of culture filtrates of Culicinomyces clavisporus: Mycoadulticide for Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi

Efficacy of the Trichophyton ajelloi and Lagenidium giganteum metabolites against mosquitoes after flash chromatography

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