Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun, Kai; Chang, Yong Keun; Zhou, Binbin; Wang, Xiaojin; Liu, Lin

doi:10.2147/ijn.s127957

Cited by 26 publications

(10 citation statements)

References 72 publications

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“…One of the most outstanding applications for metal nanoparticles is their use in labeling and imaging, especially in magnetic resonance imaging, surface-enhanced Raman scattering, and fluorescence spectroscopy [ 9 ]. Another popular use of metal nanoparticles is as optical or electrochemical biosensors [ 10 , 11 ]. The use of metal nanoparticles in such techniques enabled their involvement in the medical and clinical diagnosis of many diseases such as cancer [ 12 ], Alzheimer’s disease [ 13 ], HIV [ 14 ], hepatitis B [ 15 ], tuberculosis [ 16 ], diabetes [ 17 ], and influenza [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Cyanobacteria as Nanogold Factories: Chemical and Anti-Myocardial Infarction Properties of Gold Nanoparticles Synthesized by Lyngbya majuscula

et al. 2018

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To the best of our knowledge, cyanobacterial strains from the Arabian Gulf have never been investigated with respect to their potential for nanoparticle production. Lyngbya majuscula was isolated from the AlOqair area, Al-Ahsa Government, Eastern Province, Kingdom of Saudi Arabia. The cyanobacterium was initially incubated with 1500 mg/mL of HAuCl4 for two days. The blue-green strain turned purple, which indicated the intracellular formation of gold nanoparticles. Prolonged incubation for over two months triggered the extracellular production of nanogold particles. UV-visible spectroscopy measurements indicated the presence of a resonance plasmon band at ~535 nm, whereas electron microscopy scanning indicated the presence of gold nanoparticles with an average diameter of 41.7 ± 0.2 nm. The antioxidant and anti-myocardial infarction activities of the cyanobacterial extract, the gold nanoparticle solution, and a combination of both were investigated in animal models. Isoproterenol (100 mg/kg, SC (sub cutaneous)) was injected into experimental rats for three days to induce a state of myocardial infarction; then the animals were given cyanobacterial extract (200 mg/kg/day, IP (intra peritoneal)), gold nanoparticles (200 mg/kg/day, IP), ora mixture of both for 14 days. Cardiac biomarkers, electrocardiogram (ECG), blood pressure, and antioxidant enzymes were determined as indicators of myocardial infarction. The results showed that isoproterenol elevates ST and QT segments and increases heart rate and serum activities of creatine phosphokinase (CPK), creatine kinase-myocardial bound (CP-MB), and cardiac troponin T (cTnT). It also reduces heart tissue content of glutathione peroxidase (GRx) and superoxide dismutase (SOD), and the arterial pressure indices of systolic arterial pressure (SAP), diastolic arterial pressure (DAP), and mean arterial pressure (MAP). Gold nanoparticles alone or in combination with cyanobacterial extract produced an inhibitory effect on isoproterenol-induced changes in serum cardiac injury markers, ECG, arterial pressure indices, and antioxidant capabilities of the heart.

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Section: Introductionmentioning

confidence: 99%

Cyanobacteria as Nanogold Factories: Chemical and Anti-Myocardial Infarction Properties of Gold Nanoparticles Synthesized by Lyngbya majuscula

et al. 2018

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“…Once the peptide was bound to the target, it would lose the ability to trigger the aggregation of NPs. Based on this fact, AuNPs and AgNPs-based liquid-phase colorimetric assays have been converted into surface-tethered electrochemical electrochemical assays [71]. For example, the tripeptide (Arg-Pro-Arg) with two positively charged arginine residues could lead to the AuNPs aggregation.…”

Section: Peptide-induced Assembly Of Nanoparticlesmentioning

confidence: 99%

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

et al. 2021

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The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design of electrochemical biosensors for the signal output and amplification. In this review, we highlight the significant progress in the in situ assembly of nanomaterials as the nanolabels for enhancing the performances of electrochemical biosensors. The works are discussed based on the difference in the interactions for the assembly of nanomaterials, including DNA hybridization, metal ion–ligand coordination, metal–thiol and boronate ester interactions, aptamer–target binding, electrostatic attraction, and streptavidin (SA)–biotin conjugate. We further expand the range of the assembly units from nanomaterials to small organic molecules and biomolecules, which endow the signal-amplified strategies with more potential applications.

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“…[ 162 ] A similar method was carried out based on a kinase peptide binder for the detection of kinase with an LOD of 20 mU mL −1 . [ 163 ]…”

Section: Targeting Molecules Via Peptide–gold Hybrid Nanomaterialsmentioning

confidence: 99%

Rational Design of Functional Peptide–Gold Hybrid Nanomaterials for Molecular Interactions

et al. 2020

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Gold nanoparticles (AuNPs) have been extensively used for decades in biosensing‐related development due to outstanding optical properties. Peptides, as newly realized functional biomolecules, are promising candidates of replacing antibodies, receptors, and substrates for specific molecular interactions. Both peptides and AuNPs are robust and easily synthesized at relatively low cost. Hence, peptide–AuNP‐based bio‐nano‐technological approaches have drawn increasing interest, especially in the field of molecular targeting, cell imaging, drug delivery, and therapy. Many excellent works in these areas have been reported: demonstrating novel ideas, exploring new targets, and facilitating advanced diagnostic and therapeutic technologies. Importantly, some of them also have been employed to address real practical problems, especially in remote and less privileged areas. This contribution focuses on the application of peptide–gold hybrid nanomaterials for various molecular interactions, especially in biosensing/diagnostics and cell targeting/imaging, as well as for the development of highly active antimicrobial/antifouling coating strategies. Rationally designed peptide–gold nanomaterials with functional properties are discussed along with future challenges and opportunities.

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Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Cited by 26 publications

References 72 publications

Cyanobacteria as Nanogold Factories: Chemical and Anti-Myocardial Infarction Properties of Gold Nanoparticles Synthesized by Lyngbya majuscula

Cyanobacteria as Nanogold Factories: Chemical and Anti-Myocardial Infarction Properties of Gold Nanoparticles Synthesized by Lyngbya majuscula

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

Rational Design of Functional Peptide–Gold Hybrid Nanomaterials for Molecular Interactions

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