GPU accelerated real-time multi-functional spectral-domain optical coherence tomography system at 1300nm

Abstract

We present a GPU accelerated multi-functional spectral domain optical coherence tomography system at 1300nm. The system is capable of real-time processing and display of every intensity image, comprised of 512 pixels by 2048 A-lines acquired at 20 frames per second. The update rate for all four images with size of 512 pixels by 2048 A-lines simultaneously (intensity, phase retardation, flow and en face view) is approximately 10 frames per second. Additionally, we report for the first time the characterization of phase retardation and diattenuation by a sample comprised of a stacked set of polarizing film and wave plate. The calculated optic axis orientation, phase retardation and diattenuation match well with expected values. The speed of each facet of the multi-functional OCT CPU-GPU hybrid acquisition system, intensity, phase retardation, and flow, were separately demonstrated by imaging a horseshoe crab lateral compound eye, a non-uniformly heated chicken muscle, and a microfluidic device. A mouse brain with thin skull preparation was imaged in vivo and demonstrated the capability of the system for live multi-functional OCT visualization.

Decreased light attenuation in cerebral cortex during cerebral edema detected using optical coherence tomography

“…Real-time visualization of the sample was achieved using in-house developed software, which utilized a graphics processing unit (GPU) to process and display the OCT structure and blood flow images in real time. 14 The real-time display of OCT images allowed structural markers within the imaging location to be rapidly identified and ensured that the same region of the brain was consistently imaged during and across all experiments. Depth-resolved structural information was retrieved from each A-line by applying a Fourier transform to the spectrum collected on the camera after linearly remapping to k -space.…”

Towards real-time detection of seizures in awake rats with GPU-accelerated diffuse optical tomography

“…In the field of medical imaging, which often faces heavy computing burdens, GPU has been successfully applied in techniques like magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound imaging for image rendering, construction, and analysis 37 . In the area of optical imaging, GPU has been used in Monte Carlo simulation of photon migration 38 , fluorescence molecular tomography 39 , frequency-domain DOT 26 , frequency-domain optical coherence tomography 40,41 , and recently in photoacoustic tomography 42 .…”

In vivo detection of cortical optical changes associated with seizure activity with optical coherence tomography

“…The occurrences of the experimental steps are indicated: S: Saline injection (first red bar), PTZ: PTZ injection (second red bar), FMJ: Facial myoclonic jerks (green dashed line), FS: Full stage-5 seizure (blue dashed line). The gray region is the 2SD interval above and below the mean of the 10 min baseline.) [23]. In SD-OCT, the sensitivity of the system decreases as a function of depth due to the finite resolution of the spectrometer and because of this, we applied a depth-correction function, reported by Yun et al [24], during image processing by multiplying each A-line by the calculated correction curve.…”