Grafting increases cadmium accumulation in the post-grafting generations of the potential cadmium-hyperaccumulatorSolanum photeinocarpum

Intercropping with Solanum species decreases cadmium uptake and increases phosphorus and potassium uptakes in Amygdalus persica

“…The experiment was conducted in a greenhouse at the Chengdu Campus from March to May 2021. Soil samples were treated according to Lin et al 27 The Cd was added as CdCl 2 ·2.5H 2 O to make a final soil concentration of 5 mg kg −1. 17,18 Each pot (diameter of 25 cm and 10 cm depth) contained 3.0 kg of well‐mixed Cd‐contaminated soil.…”

“…The experiment was conducted in a greenhouse at the Chengdu Campus from March to May 2021. Soil samples were treated according to Lin et al 27 The Cd was added as CdCl 2 Á2.5H 2 O to make a final soil concentration of 5 mg kg À1.17 growth, all pots were watered every day to keep the soil moisture content maintaining at 80% field capacity, the other weeds were removed timely, and the pests were controlled timely.…”

“…After 40 days of cultivation in the Cd-contaminated soil (May 2020), 17,18 all plants were harvested and treated accordingly to the study of Lin et al 27 The root, stem, and leaf biomass (dry weight) were measured using an electronic balance (accuracy, 0.001 g). The Cd contents in roots, stems, and leaves were determined using the iCAP 6300 ICP-MS spectrometer (Thermo Scientific, MA, USA) following the report of Lin et al 27 The translocation factor (TF) was calculated as Cd content in shoots/Cd content in roots, and the Cd extraction of hyperaccumulator was calculated as plant Cd content  plant biomass. 28 Dry plant samples were digested in H 2 SO 4 and H 2 O 2 to determine the total P and total K contents using the spectrophotometer and flame photometer, respectively.…”

“…29 At the end of the experiment, potted soil was collected, air-dried, and sieved through a 1-mm mesh. The soil pH value was determined using a pH meter, and the soil exchangeable Cd concentration was determined using the iCAP 6300 ICP-MS spectrometer following the report of Lin et al 27 The soil available P concentration was extracted using NaHCO 3 and determined using a spectrophotometer, and the soil available K concentration was extracted using NH 4 OAc and determined using a flame photometer. 29…”

Application of uniconazole (S3307) promotes selenium accumulation inCyphomandra betaceaseedlings under selenium stress

“…The SOD, POD, and CAT activities were determined by the nitroblue tetrazolium photoreduction method, guaiacol colorimetric method, and potassium permanganate titration method, respectively; the soluble protein content was determined by Coomassie brilliant blue staining. The details of determining the antioxidant enzyme activity and soluble protein content were described in the previous studies 29,30 . After that, all plants were harvested and treated as reported by Lin et al (2020), 30 and the root, stem, and leaf biomass (dry weight) were determined using an electronic balance.…”