Gramella aestuarii sp. nov., isolated from a tidal flat, and emended description of Gramella echinicola

Abstract

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterial strain motile by gliding, designated BS12 T , was isolated from a tidal flat at Boseong, Korea. Cells were moderately halotolerant, catalase-and oxidase-positive rods. Growth was observed at 5-40 6C (optimum, 25 6C), at pH 5.5-9.0 (optimum, pH 7.0-7.5) and in the presence of 1-11 % (w/v) NaCl (optimum, 2-4 %). The major cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH, iso-C 17 : 1 v9c and anteiso-C 15 : 0 . The polar lipid pattern comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 42.3 mol% and the only respiratory quinone was menaquinone 6. Phylogenetic inference based on 16S rRNA gene sequences showed that strain BS12 T formed a tight phyletic lineage with members of the genus Gramella. Strain BS12 T was most closely related to 'Gramella jeungdoensis' HMD3159 with sequence similarity of 97.5 %, but the DNA-DNA relatedness value between the two strains was only 22.1±2.2 %. On the basis of phenotypic and molecular features, strain BS12 T was shown to represent a novel species of the genus Gramella, for which the name Gramella aestuarii sp. nov. is proposed. The type strain is BS12 T (5KACC 16188 T 5JCM 17790 T ). An emended description of Gramella echinicola is also proposed.

Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment

“…Similarly, none of the strains possessed polar lipids such as PG, DPG and SGL. Present results substantially validated the polar lipid data previously reported by Jeong et al (2013) for G. echinicola KCTC 12278…”

Gramella aestuariivivens sp. nov., isolated from a tidal flat

“…The genus Gramella , a member of the family Flavobacteriaceae of the phylum Bacteroidetes , was proposed by Nedashkovskaya et al (2005) with the description of a single recognized species, Gramella echinicola , isolated from the sea urchin Strongylocentrotus intermedius collected from Troitsa Bay, Gulf of Peter the Great, Sea of Japan. Subsequently, seven further species of the genus Gramella with validly published names, Gramella portivictoriae (Lau et al , 2005), Gramella marina (Nedashkovskaya et al , 2010), Gramella gaetbulicola (Cho et al , 2011), Gramella aestuarii (Jeong et al , 2013), Gramella flava (Liu et al , 2014), Gramella oceani (Hameed et al , 2014) and Gramella planctonica (Shahina et al , 2014a, b), have been described. Members of the genus Gramella have been isolated from marine organisms and marine environments (Nedashkovskaya et al , 2005, 2010; Lau et al , 2005; Cho et al , 2011; Jeong et al , 2013; Hameed et al , 2014; Liu et al , 2014).…”

Gramella aquimixticola sp. nov., isolated from water of an estuary environment

“…The genus Gramella , a member of the family Flavobacteriaceae of the phylum Bacteroidetes (Bernardet, 2011), was proposed by Nedashkovskaya et al (2005) with the description of a single recognized species, Gramella echinicola . At the time of writing, the genus Gramella comprises nine species with validly published names, G. echinicola (Nedashkovskaya et al , 2005), Gramella portivictoriae (Lau et al , 2005), Gramella marina (Nedashkovskaya et al , 2010), Gramella gaetbulicola (Cho et al , 2011), Gramella aestuarii (Jeong et al , 2013), Gramella flava (Liu et al , 2014), Gramella oceani (Hameed et al , 2014), Gramella planctonica (Shahina et al , 2014a, b) and Gramella aestuariivivens (Park et al , 2015). All members of the genus Gramella have been isolated from marine environments (Lau et al , 2005; Nedashkovskaya et al , 2005, 2010; Cho et al , 2011; Jeong et al , 2013; Hameed et al , 2014; Liu et al , 2014; Shahina et al , 2014a; Park et al , 2015).…”

“… * Data from: a , Jeong et al (2013); b , Park et al (2015); c , Nedashkovskaya et al (2005); d , Cho et al (2011); e , Nedashkovskaya et al (2010); f , Lau et al (2005). …”