Graphene nanoplatelets spontaneously translocate into the cytosol and physically interact with cellular organelles in the fish cell line PLHC-1

Lammel, Tobias; Navas, José M.

doi:10.1016/j.aquatox.2014.02.016

Cited by 54 publications

(55 citation statements)

References 51 publications

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“…Oyster hemocytes are immune cells that can phagocytose foreign particles and can also produce ROS (Boyd and Burnett 1999). In agreement with our findings, other studies have also reported physical damage by GFNs to cellular membranes (Lammel and Navas 2014;Chen et al 2016), suggesting critical risks to cell function and viability. Further, an interdependent relationship between inflammation and oxidative stress has been suggested in nanomaterial toxicity (Manke et al 2013).…”

Section: Histopathological Assessmentsupporting

confidence: 93%

A 72‐h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide

Khan

Adeleye

Burgess

et al. 2019

Enviro Toxic and Chemistry

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Graphene is a 2‐dimensional nanomaterial with unique mechanical, thermal, electrical, and optical properties. With increasing applications of graphene‐family nanomaterials (GFNs) in electronics, biomedicine, and surface coatings, concern for their impacts on aquatic ecosystems is rising. Current information on the toxicity of GFNs, including graphene oxide, is scarce. Filter‐feeding bivalves, such as eastern oysters, are good models for nanomaterial exposure studies. We present results from a 72‐h static renewal oyster study using 1 and 10 mg/L graphene oxide, which, to our knowledge, is the first report on in vivo effects of graphene oxide exposures in marine bivalves. Water samples were analyzed for graphene oxide concentration and size assessments. Gill and digestive gland tissues were evaluated for lipid peroxidation and glutathione‐S‐transferase (GST) activity. In addition, gill sections were fixed for histopathological analyses. Elevated lipid peroxidation was noted in oysters exposed to 10 mg/L graphene oxide. No significant changes in GST activity were observed, but reduced total protein levels were found in digestive gland tissues of exposed oysters at both concentrations. Loss of mucous cells, hemocytic infiltration, and vacuolation were observed in gills of exposed oysters. The results indicate that short‐term graphene oxide exposures can induce oxidative stress and epithelial inflammation and adversely affect overall oyster health. Further investigations regarding the fate and sublethal effects of graphene oxide are critical to understanding the risks associated with a rapidly growing graphene consumer market. Environ Toxicol Chem 2019;38:820–830. Published 2019 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.

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Section: Histopathological Assessmentsupporting

confidence: 93%

A 72‐h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide

Khan

Adeleye

Burgess

et al. 2019

Enviro Toxic and Chemistry

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“…Among GPN, pG, GQD, GO, GO nanosheets, GO flakes, single layer GO, rGO and rGOQD were used; in addition, several NOM, including HA, CYS, bwGO, and GOBS potentially modulate the effects of GBNs on zebrafish (Table 2). Although in this review, we have restricted our observations on embryos, larvae, and adult fish, several fish cell lines, including the hepatocellular carcinoma cell line PLHC-1 (derived from topminnow fish, Poeciliopsis lucida ) (Lammel and Navas, 2014), cardiac cell lines (SICH) of Catla catla (Xing et al 2016) and bluegill sunfish cells (BF-2) (Srikanth et al 2018) have been used for the evaluation of GPN toxicity (GO and RGO for SICH cell lines and PLHC1 and BF-2 for GO). From the available literature we reviewed we found that the size, shape, surface properties and chemistry, concentration, agglomeration, dose, and method of preparation of GPN, are the major determinants that affect the various biological activities in zebrafish (Chen et al 2015a, b; 2016; Zhu et al, 2015; Ren et al 2016; Lu et al 2017; Zhang et al 2017a; Zhang et al 2017b; De Marchi et al 2018).…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Graphene-Based Nanomaterials Toxicity in Fish

Dasmahapatra

Dasari

Tchounwou

2018

Reviews of Environmental Contamination and Toxicology

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Due to their unique physicochemical properties, graphene-based nanoparticles (GPN) constitute one of the most promising types of nanomaterials used in biomedical research. GPN have been used as polymeric conduits for nerve regeneration, carriers for targeted drug delivery, and in the treatment of cancer via photo-thermal therapy. Moreover, they have been used as tracers to study the distribution of bioactive compounds used in healthcare. Due to their extensive use, GPN released into the environment would probably pose a threat to living organisms and ultimately to human health. Their accumulation in the aquatic environment creates problems to aquatic habitats as well as to food chains. Until now the potential toxic effects of GPN are not properly understood. Despite agglomeration and long persistence in the environment, GPN are able to cross the cellular barriers successfully, entered into the cells and able to interact with all most all the cellular sites including the plasma membrane, cytoplasmic organelles, and nucleus. Their interaction with DNA creates more potential threats to both the genome and epigenome. In this brief review, we focused on fish, mainly zebrafish (Danio rerio), as a potential target animal of GPN toxicity in the aquatic ecosystem.

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“…253 Reprinted with permission from ref. 253. Copyright 2012 Elsevier Ltd. (h) After initial internalization by peritoneal macrophages, GO sheets of lateral size 2 µm undergo significant deformation and exhibit wrinkling.…”

Section: Figurementioning

confidence: 99%

Biological and environmental interactions of emerging two-dimensional nanomaterials

Wang¹,

Zhu²,

Qiu³

et al. 2016

Chem. Soc. Rev.

240

210

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Two-dimensional materials have become a major focus in materials chemistry research worldwide with substantial efforts centered on synthesis, property characterization, and technological application. These high-aspect ratio sheet-like solids come in a wide array of chemical compositions, crystal phases, and physical forms, and are anticipated to enable a host of future technologies in areas that include electronics, sensors, coatings, barriers, energy storage and conversion, and biomedicine. A parallel effort has begun to understand the biological and environmental interactions of synthetic nanosheets, both to enable the biomedical developments and to ensure human health and safety for all application fields. This review covers the most recent literature on the biological responses to 2D materials and also draws from older literature on natural lamellar minerals to provide additional insight into the essential chemical behaviors. The article proposes a framework for more systematic investigation of biological behavior in the future, rooted in fundamental materials chemistry and physics. That framework considers three fundamental interaction modes: (i) chemical interactions and phase transformations, (ii) electronic and surface redox interactions, and (iii) physical and mechanical interactions that are unique to near-atomically-thin, high-aspect-ratio solids. Two-dimensional materials are shown to exhibit a wide range of behaviors, which reflect the diversity in their chemical compositions, and many are expected to undergo reactive dissolution processes that will be key to understanding their behaviors and interpreting biological response data. The review concludes with a series of recommendations for high-priority research subtopics at the “bio-nanosheet” interface that we hope will enable safe and successful development of technologies related to two-dimensional nanomaterials.

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Graphene nanoplatelets spontaneously translocate into the cytosol and physically interact with cellular organelles in the fish cell line PLHC-1

Cited by 54 publications

References 51 publications

A 72‐h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide

A 72‐h exposure study with eastern oysters (Crassostrea virginica) and the nanomaterial graphene oxide

Graphene-Based Nanomaterials Toxicity in Fish

Biological and environmental interactions of emerging two-dimensional nanomaterials

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