Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification

Zhao, Xu-Hua; Ma, Qiujuan; Wu, Xiangxiang; Zhu, Xin

doi:10.1016/j.aca.2012.03.044

Cited by 82 publications

(35 citation statements)

References 32 publications

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“…Therefore, in the presence of the target DNA, a large number of probe DNA can be degraded, generating free fluorophore that are not adsorbed by GO. This sensor exhibits a high sensitivity towards the target DNA with a detection limit of 20 pM, which was better than the GO-based DNA sensors without enzymatic amplification, and it provides a universal sensing platform for sensitive detection of DNA [129]. Figure 11.…”

Section: Signal Amplification Methodsmentioning

confidence: 99%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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In the past few years, graphene oxide (GO) has emerged as a unique platform to develop DNA-based biosensors. This takes advantage of the DNA adsorption and fluorescence quenching property of GO.The adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing fluorescence signal. In addition to this initial design, many other strategies have been reported including the use of aptamers, molecular beacons, and DNAzymes as probes, label-free detection, using the intrinsic fluorescence of GO and covalently linked DNA probes. The potential applications of DNA-functionalized GO range from environmental monitoring, cell imaging and biomedical diagnosis. In this review, we first summarize the fundamental surface interactions between DNA and GO and the related fluorescence quenching mechanism. Following that, the various sensor design strategies are critically compared. Problems at the current stages are described and a few future directions are also discussed.

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Section: Signal Amplification Methodsmentioning

confidence: 99%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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“…The fabrication process of the ECL DNA biosensor was measured by cyclic voltammogram (CV) in 0.1 M KCl containing 1 mM K 3 [Fe(CN) 6 ]/K 4 [Fe(CN) 6 ]. As shown in Fig.…”

Section: The Electrochemical Characterization Of the Biosensor Fabricmentioning

confidence: 99%

“…Consequently, numerous analytical methods such as fluorescence [6,7], chemiluminescence [8][9][10], surface plasmon resonance [11,12], electrochemical techniques [13][14][15], and electrogenerated chemiluminescence (ECL) [16][17][18][19][20][21] have been developed for the detection of DNA sequences. Unfortunately, those methods are usually fit for DNA with less than ∼30 base pairs.…”

Section: Introductionmentioning

confidence: 99%

Electrogenerated chemiluminescence energy transfer and its application in label-free sensing long DNA

Guo

Kong

Yang

et al. 2015

Sensors and Actuators B: Chemical

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“…Moreover, GO offers abundant carboxyl, hydroxyl, and epoxy groups on the surface of a two-dimensional layered carbon sheet from a graphene precursor [19]. It can provide unique characteristics such as via -stacking and weak electrostatic attractions between single-stranded DNA (ssDNA) and the GO surface [20,21]. This is a key factor of enhancing the sensitivity of fluorescence-based DNA detection system.…”

Section: Introductionmentioning

confidence: 99%