Graphene oxide-based magnetic nanocomposites for the delivery of melittin to cervical cancer HeLa cells

Qi, Jinxia; Chen, Yonghui; Xue, Tiantian; Lin, Yue; Huang, Sisi; Cao, Shuyan; Wang, Xiaona; Su, Yu; Lin, Zhenkun

doi:10.1088/1361-6528/ab5084

Cited by 32 publications

(21 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whereas PEGylated GO ensured protection of melittin, Fe 3 O 4 NPs functioned as magnetic responders. Consequently, active drug was released up to 72 h and effectively inhibited the HeLa cells [218].…”

Section: Nanocomposites Of Cs-coated Fe 3 O 4 Sio 2 and Go (Cs/fe 3mentioning

confidence: 99%

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

2021

View full text Add to dashboard Cite

Carbon is one of the most abundant elements on Earth. In addition to the well-known crystallographic modifications such as graphite and diamond, other allotropic carbon modifications such as graphene-based nanomaterials and carbon nanotubes have recently come to the fore. These carbon nanomaterials can be designed to help deliver or target drugs more efficiently and to innovate therapeutic approaches, especially for cancer treatment, but also for the development of new diagnostic agents for malignancies and are expected to help combine molecular imaging for diagnosis with therapies. This paper summarizes the latest designed drug delivery nanosystems based on graphene, graphene quantum dots, graphene oxide, reduced graphene oxide and carbon nanotubes, mainly for anticancer therapy.

show abstract

Section: Nanocomposites Of Cs-coated Fe 3 O 4 Sio 2 and Go (Cs/fe 3mentioning

confidence: 99%

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

2021

View full text Add to dashboard Cite

show abstract

“…Figure 4 A shows the illustration of adsorption and separation of Pb 2+ from an aqueous solution [ 57 ]. Qi et al synthesized the GO-based magnetic nanocomposites (PEG-GO-Fe 3 O 4 ) for melittin drug release [ 58 ]. The controlled release of melittin was observed and maintained its inhibition effect on HeLa cells.…”

Section: Stimuli-responsive Factorsmentioning

confidence: 99%

Graphene Oxide-Based Stimuli-Responsive Platforms for Biomedical Applications

et al. 2021

View full text Add to dashboard Cite

Graphene is a two-dimensional sp2 hybridized carbon material that has attracted tremendous attention for its stimuli-responsive applications, owing to its high surface area and excellent electrical, optical, thermal, and mechanical properties. The physicochemical properties of graphene can be tuned by surface functionalization. The biomedical field pays special attention to stimuli-responsive materials due to their responsive abilities under different conditions. Stimuli-responsive materials exhibit great potential in changing their behavior upon exposure to external or internal factors, such as pH, light, electric field, magnetic field, and temperature. Graphene-based materials, particularly graphene oxide (GO), have been widely used in stimuli-responsive applications due to their superior biocompatibility compared to other forms of graphene. GO has been commonly utilized in tissue engineering, bioimaging, biosensing, cancer therapy, and drug delivery. GO-based stimuli-responsive platforms for wound healing applications have not yet been fully explored. This review describes the effects of different stimuli-responsive factors, such as pH, light, temperature, and magnetic and electric fields on GO-based materials and their applications. The wound healing applications of GO-based materials is extensively discussed with cancer therapy and drug delivery.

show abstract

“…Graphene oxide (GO) nanoparticles (NPs) have been widely used in biomedical fields due to their physical and chemical properties, which make them useful for applications in as drug delivery [1,2], tumor photothermal therapy [3][4][5], bioimaging [6], tissue engineering [7] antimicrobial agents [8], biosensors [9][10][11]. At the same time, the risk of human contact has increased dramatically.…”

Section: Introductionmentioning

confidence: 99%

The interrupted effect of autophagic flux and lysosomal function induced by graphene oxide in p62-dependent apoptosis of F98 cells

Zhang

Feng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Graphene oxide (GO) nanoparticles (NPs) have been widely applied in various fields, especially in biomedical applications. Extensive studies have suggested that GO can pass through the blood-brain barrier (BBB) and induce abnormal autophagy and cytotoxicity in the central nervous system (CNS). However, the effect and specific mechanism of GO on astrocytes, the most abundant cells in the brain still has not been extensively investigated. Results In this study, we systematically explored the toxicity and mechanism of GO exposure in the rat astroglioma-derived F98 cell line using molecular biological techniques (immunofluorescence staining, flow cytometry and Western blot) at the subcellular level and the signaling pathway level. Cells exposed to GO exhibited decreased cell viability and increased lactate dehydrogenase (LDH) release in a concentration- and time-dependent manner. GO-induced autophagy was evidenced by transmission electron microscopy (TEM) and immunofluorescence staining. Western blots showed that LC3II/I and p62 were upregulated and PI3K/Akt/mTOR was downregulated. Detection of lysosomal acidity and cathepsin B activity assay indicated the impairment of lysosomal function. Annexin V-FITC-PI detection showed the occurrence of apoptosis after GO exposure. The decrease in mitochondrial membrane potential (MMP) with an accompanying upregulation of cleaved caspase-3 and Bax/Bcl-2 further suggested that endogenous signaling pathways were involved in GO-induced apoptosis. Conclusion GO-treated cells exhibited autophagy activation by inhibition of the PI3K/Akt/mTOR signaling pathway. Simultaneously, GO can induce the dysfunction of lysosomes via alkalization and then block autophagic flux, resulting in the abnormal accumulation of autophagic substrate p62 protein. Abnormal accumulation of p62 protein can induce capase-3-mediated apoptosis. The systematic exploration of GO in F98 cells in this study provides insight for the biocompatible application of graphene-family NPs in the CNS.

show abstract

Graphene oxide-based magnetic nanocomposites for the delivery of melittin to cervical cancer HeLa cells

Cited by 32 publications

References 62 publications

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

Graphene Oxide-Based Stimuli-Responsive Platforms for Biomedical Applications

The interrupted effect of autophagic flux and lysosomal function induced by graphene oxide in p62-dependent apoptosis of F98 cells

Contact Info

Product

Resources

About