Graphene oxide nanosheets induce DNA damage and activate the base excision repair (BER) signaling pathway both in vitro and in vivo

Lu, Chihua; Jiang, Xuefeng; Junaid, Muhammad; Ma, Yanbo; Jia, Pan‐Pan; Wang, Huabin; Pei, De‐Sheng

doi:10.1016/j.chemosphere.2017.06.049

Cited by 58 publications

(46 citation statements)

References 53 publications

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“…So, the impact of GPN either in genome and epigenome at specific stages of development cannot be understood by simply exposing the fertilized embryos for the entire period of development (both in ovo and ex ovo ). For larvae (Ren et al 2016) and adults (Lu et al 2017) the duration of exposure ranges from 1–14 day depending on the nature of the experiment. Although the absorption sites are mainly through gills in adults, accumulation of GPN may occur in the liver, brain, intestine and other organs of the body (Lu et al 2016).…”

Section: Uptake and Biodistribution Of Gpn In Embryos Larvae And Admentioning

confidence: 99%

“…However, the incorporation by immersion either in embryos, larvae, or adults, the nanomaterial, even at the ultralow concentration (0.01–1μg/L) is able to reach equilibrium and can show organ specific distribution rather than the uniform distribution in the entire body as observed by microinjection. On the other hand, incorporation through waterborne exposure depends on the size, concentration of GPN, time of exposure, developmental stage, as well as the presence of different types of NOM in the environment (Lu et al 2017; Su et al, 2017) (Table 1). The s-FLG is more potent in crossing the chorion in the embryos (Su et al 2017) or in the organs of adult zebrafish (Lu et al, 2017).…”

Section: Uptake and Biodistribution Of Gpn In Embryos Larvae And Admentioning

confidence: 99%

“…In larvae, at 96 hpf, GO-FITC reached fish mouth, yolk sac, cardiac region, and tail blood within 1 h of incubation (Zhang et al, 2017b). However, in adult fish, maximum accumulation of s-FLG and l-FLG was reached at 48 h of exposure (Lu et al, 2017). These studies indicate that the developmental stage (age) of fish has a significant influence on GPN accumulation.…”

Section: Uptake and Biodistribution Of Gpn In Embryos Larvae And Admentioning

confidence: 99%

“…Blastula stages of zebrafish embryos were exposed to different concentrations (5, 25, and 50 μg/mL) of GO (height ∼1.5 nm; lateral size 1500 nm) for 12, 24, 48, and 72 hpf (Lu et al, 2017). The survival rates of the embryos were decreased with all GO concentrations within first 24 h of exposure and remained constant thereafter (from 48–72 h).…”

Section: Toxic Effects Of Gpnmentioning

confidence: 99%

“…Due to excellent morphological and biological features, Lin et al (2013) proposed to use zebrafish ( Danio rerio ) as an in vivo model for the study of environmental health and safety (EHS) aspects of engineered nanomaterials and nano-related products. Since then several excellent studies based on accumulation, morphology, behavior, neurological disorders, molecular mechanisms, and other potential targets of GPN have been conducted on embryos, larvae, adults, and transgenic zebrafish (Liu et al 2014; Chen et al 2015a,b; Jiang et al 2015; Mu et al 2015; Wang et al 2015; Ren et al 2016; d’Amora et al 2017; Li et al 2017; Lu et al 2017; Soares et al 2017; Souza et al 2017; Zhang et al 2017a; Zhang et al, 2017b). Studies have also been carried out with other fish species (Japanese medaka; Oryzias latipes ) (Mullick Chowdhury et al 2014; Li et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Graphene-Based Nanomaterials Toxicity in Fish

Dasmahapatra

Dasari

Tchounwou

2018

Reviews of Environmental Contamination and Toxicology

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Due to their unique physicochemical properties, graphene-based nanoparticles (GPN) constitute one of the most promising types of nanomaterials used in biomedical research. GPN have been used as polymeric conduits for nerve regeneration, carriers for targeted drug delivery, and in the treatment of cancer via photo-thermal therapy. Moreover, they have been used as tracers to study the distribution of bioactive compounds used in healthcare. Due to their extensive use, GPN released into the environment would probably pose a threat to living organisms and ultimately to human health. Their accumulation in the aquatic environment creates problems to aquatic habitats as well as to food chains. Until now the potential toxic effects of GPN are not properly understood. Despite agglomeration and long persistence in the environment, GPN are able to cross the cellular barriers successfully, entered into the cells and able to interact with all most all the cellular sites including the plasma membrane, cytoplasmic organelles, and nucleus. Their interaction with DNA creates more potential threats to both the genome and epigenome. In this brief review, we focused on fish, mainly zebrafish (Danio rerio), as a potential target animal of GPN toxicity in the aquatic ecosystem.

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Section: Uptake and Biodistribution Of Gpn In Embryos Larvae And Admentioning

confidence: 99%

Section: Uptake and Biodistribution Of Gpn In Embryos Larvae And Admentioning

confidence: 99%

Section: Uptake and Biodistribution Of Gpn In Embryos Larvae And Admentioning

confidence: 99%

Section: Toxic Effects Of Gpnmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Graphene-Based Nanomaterials Toxicity in Fish

Dasmahapatra

Dasari

Tchounwou

2018

Reviews of Environmental Contamination and Toxicology

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Systems toxicology assessment revealed the impact of graphene‐based materials on cell cycle regulators

Farahani

Rezaei‐Tavirani

Zali

et al. 2020

J Biomedical Materials Res

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Understanding the cellular and molecular toxicity of graphene and its derivatives is essential for their biomedical applications. Herein, gene expression profile of graphene-exposed cells was retrieved from the Gene expression omnibus database.Differentially expressed genes and their functional roles were then investigated through the pathway, protein-protein interaction (PPI) network, and module analysis.High degree (hub) and high betweenness centrality (bottleneck) nodes were subsequently identified. The functional analysis of central genes indicated that these graphene-gene interactions could be of great value for further investigation. Accordingly, we also followed the expression of five hub-bottleneck genes in graphenetreated murine peritoneal macrophages and human breast cancer cell line by realtime PCR. The five hub-bottleneck genes related to graphene cytotoxicity; CDK1, CCNB1, PLK1, TOP2A, and CCNA2 were identified through network analysis, which were highly correlated with regulation of cell cycle processes. The module analysis indicated the cell cycle pathway to be the predominant one. Gene expression evaluation showed downregulation of these genes in the macrophages and cancer cells treated with graphene. These results provided some new intuitions concerning the graphene-cell interactions and unveiled targeting critical cell cycle regulators. The present study indicated some toxic effects of graphene-based materials through systems toxicology assessment. Integrating gene expression and PPI network may help explaining biological responses of graphene and lead to beneficial impacts in nanomedicine. K E Y W O R D Scell cycle, gene expression, graphene, protein-protein interaction network, systems toxicology

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Responses of flocculent and granular sludge in anaerobic sequencing batch reactors (ASBRs) to azithromycin wastewater and its impact on microbial communities

Liu

Chen

Shao

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUNDAzithromycin (AZM), a 15‐member macrolide antibiotic, has caused widespread pollution in China, where anaerobic digestion is the predominant wastewater treatment technology. However, the type of anaerobic sludge most suitable for treating antibiotic wastewater is unclear. Therefore, this study aimed to determine the response of anaerobic flocculent and granular sludge to AZM wastewater, and, using a high‐throughput sequencing technique, to explain the diverse microbial community structures.RESULTSAnaerobic granular sludge provided better resistance to the decline of chemical oxygen demand (COD) removal caused by AZM and greater biodegradation efficiency than flocculent sludge. Furthermore, there was stronger resistance to AZM‐induced reactive oxygen species in the granular sludge than in the flocculent sludge. The granular sludge exhibited richer microbial communities than the flocculent sludge after AZM exposure to the phylum Proteobacteria and Bacteroidetes.CONCLUSIONAt high AZM concentration, anaerobic granular sludge was superior to flocculent sludge in COD removal and AZM degradation. This is attributed to less cell death and greater microbial diversity and richness in the granular sludge than in the flocculent sludge following exposure to AZM. Proteobacteria and Bacteroidetes contributed to the resistance to AZM in the granular sludge. © 2018 Society of Chemical Industry

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Graphene oxide nanosheets induce DNA damage and activate the base excision repair (BER) signaling pathway both in vitro and in vivo

Cited by 58 publications

References 53 publications

Graphene-Based Nanomaterials Toxicity in Fish

Graphene-Based Nanomaterials Toxicity in Fish

Systems toxicology assessment revealed the impact of graphene‐based materials on cell cycle regulators

Responses of flocculent and granular sludge in anaerobic sequencing batch reactors (ASBRs) to azithromycin wastewater and its impact on microbial communities

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