Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Stephan, Chadi H.; Fournier, Michel; Brousseau, Pauline; Sauvé, Sébastien

doi:10.1186/1752-153x-2-14

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…However, several of the membrane samples from this series of studies would have been near or below the limits of detection for GFAAS (Stephan et al, 2008). To our knowledge, this is the first time that AMS has been utilized to detect a metal antigen processed by a cell system.…”

Section: Discussionmentioning

confidence: 98%

Accelerator mass spectrometry detection of beryllium ions in the antigen processing and presentation pathway

Tooker

Brindley

Chiarappa-Zucca

et al. 2014

Journal of Immunotoxicology

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Exposure to small amounts of beryllium (Be) can result in beryllium sensitization and progression to Chronic Beryllium Disease (CBD). In CBD, beryllium is presented to Be-responsive T-cells by professional antigen-presenting cells (APC). This presentation drives T-cell proliferation and pro-inflammatory cytokine (IL-2, TNFα, and IFNγ) production and leads to granuloma formation. The mechanism by which beryllium enters an APC and is processed to become part of the beryllium antigen complex has not yet been elucidated. Developing techniques for beryllium detection with enough sensitivity has presented a barrier to further investigation. The objective of this study was to demonstrate that Accelerator Mass Spectrometry (AMS) is sensitive enough to quantify the amount of beryllium presented by APC to stimulate Be-responsive T-cells. To achieve this goal, APC - which may or may not stimulate Be-responsive T-cells - were cultured with Be-ferritin. Then, by utilizing AMS, the amount of beryllium processed for presentation was determined. Further, IFNγ intracellular cytokine assays were performed to demonstrate that Be-ferritin (at levels used in the experiments) could stimulate Be-responsive T-cells when presented by an APC of the correct HLA type (HLA-DP0201). The results indicated that Be-responsive T-cells expressed IFNγ only when APC with the correct HLA type were able to process Be for presentation. Utilizing AMS, it was determined that APC with HLA-DP0201 had membrane fractions containing 0.17-0.59 ng Be and APC with HLA-DP0401 had membrane fractions bearing 0.40-0.45 ng Be. However, HLA-DP0401 APC had 20-times more Be associated with the whole cells (57.68-61.12 ng) then HLA-DP0201 APC (0.90-3.49 ng). As these findings demonstrate, AMS detection of picogram levels of Be processed by APC is possible. Further, regardless of form, Be requires processing by APC to successfully stimulate Be-responsive T-cells to generate IFNγ.

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Section: Discussionmentioning

confidence: 98%

Accelerator mass spectrometry detection of beryllium ions in the antigen processing and presentation pathway

Tooker

Brindley

Chiarappa-Zucca

et al. 2014

Journal of Immunotoxicology

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“…Cu and Se in all the examined organs were classified into the group of trace elements. The TXRF results, presented in the Table 5, pointed also at high variability in (5) www.nature.com/scientificreports/ the concentrations of the studied elements in tissues taken from individual animals. The high population variability concerned, mainly, the accumulation of Ca and Zn, and in some organs also Fe.…”

Section: Basis Of Quantitative Elemental Analysismentioning

confidence: 92%

The assessment of the usability of selected instrumental techniques for the elemental analysis of biomedical samples

Planeta

Kubala‐Kukuś

Dróżdż

et al. 2021

Sci Rep

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The fundamental role of major, minor and trace elements in different physiological and pathological processes occurring in living organism makes that elemental analysis of biomedical samples becomes more and more popular issue. The most often used tools for analysis of the elemental composition of biological samples include Flame and Graphite Furnace Atomic Absorption Spectroscopy (F-AAS and GF-AAS), Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Each of these techniques has many advantages and limitations that should be considered in the first stage of planning the measurement procedure. Their reliability can be checked in the validation process and the precision, trueness and detection limits of elements belong to the most frequently determined validation parameters. The main purpose of this paper was the discussion of selected instrumental techniques (F-AAS, GF-AAS, ICP-OES and ICP-MS) in term of the achieved validation parameters and the usefulness in the analysis of biological samples. The focus in the detailed literature studies was also put on the methods of preparation of the biomedical samples. What is more based on the own data the usefulness of the total reflection X-ray fluorescence spectroscopy for the elemental analysis of animal tissues was examined. The detection limits of elements, precision and trueness for the technique were determined and compared with the literature data concerning other of the discussed techniques of elemental analysis. Reassuming, the following paper is to serve as a guide and comprehensive source of information concerning the validation parameters achievable in different instrumental techniques used for the elemental analysis of biomedical samples.

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“…Se and Co measurements in acidic aqueous dilution (blanks, samples, and calibration curve) were performed with an atomic absorption spectrophotometer (AAS Perkin Elmer, Analyst 300, USA) equipped with a deuterium lamp background corrector, a Perkin Elmer HGA 800, USA, graphite furnace, and a Perkin Elmer AS-800 autosampler, USA [13,14]. The determinations were conducted using matrix modifiers based on magnesium nitrate and palladium nitrate [15].…”

Section: Sample Preparationmentioning

confidence: 99%

Macrominerals, trace elements and hem and non–hem iron status in muscle Longissimus dorsi, from five double purpose lambs breed reared on pasture system in Uruguay

Guerra,

Moreni,

Saadoun

et al. 2024

RC FCV-LUZ

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Sheep meat production is facing new challenges, so a thorough knowledge of the attributes of lamb meat produced by different genotypes and under pasture conditions is necessary to characterise these systems, to valorise and differentiate the product from a quality approach and towards a more natural image, attributes that are increasingly taken into account by consumers. This study aimed to characterize the lamb meat nutritionally, coming from five genetic types, reared in a pastoral system, through the content of essential minerals, macro element, Ca, Mg, Na and K, trace elements as Se, Co, Zn, Cu, Mn, total iron (TFe), hem iron (HFe) and non–hem iron (NHFe) and B12 vitamin in the Longissimus dorsi muscle. The breeds, Corriedale, Merino Dohne, Highlander®, Corriedale Pro, and Australian Merino x Corriedale crossbreed; n=10, were studied. Merino Dohne breed has the highest calcium concentration (66.6 ± 6.3 mg·kg–1), Highlander® and Merino Dohne have a significantly (P<0.05) higher manganese concentration (304.1 ± 26.0 and 308.7 ± 23.6 µg·kg–1, respectively) than the other breeds. There were no significant differences in vitamin B12 concentrations between lamb breeds. The HFe and HFe/TFe ratio was higher (P<0.05) in the Corriedale and Corriedale Pro breeds (15.7 ± 0.6 and 15.4 ± 0.7 mg·kg–1 and 81.7 ± 2.8% and 76.0 ± 2.2%, respectively) and consequently less NHFe, related to others groups. Also, increased Zn content was obtained in Corriedale (32.6 ± 1.3 mg·kg–1), but other breeds are also rich in zinc. These results show that meat from these breeds qualifies as a good source claim for people with high requirements as children and elders.

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Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Cited by 11 publications

References 36 publications

Accelerator mass spectrometry detection of beryllium ions in the antigen processing and presentation pathway

Accelerator mass spectrometry detection of beryllium ions in the antigen processing and presentation pathway

The assessment of the usability of selected instrumental techniques for the elemental analysis of biomedical samples

Macrominerals, trace elements and hem and non–hem iron status in muscle Longissimus dorsi, from five double purpose lambs breed reared on pasture system in Uruguay

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