Graphite paste-based enzymatic glucose electrode for flow injection analysis

Matuszewski, Wojciech; Trojanowicz, Marek

doi:10.1039/an9881300735

Cited by 74 publications

(31 citation statements)

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“…O primeiro eletrodo de pasta de carbono 8 foi desenvolvido, em 1958, na tentativa de preparar um eletrodo de carbono renovável para ser usado em uma ampla faixa de potenciais positivos onde o eletrodo de mercúrio metálico não é aplicável devido à sua oxidação. No entanto, o primeiro eletrodo de pasta de carbono modificado com enzima (glicose oxidase) foi proposto apenas no final da déca-da de 80 9 , e desde então vários biossensores foram desenvolvidos usando diversos materiais biológicos [10][11][12][13][14][15][16] . A pasta de carbono é uma mistura de pó de grafite e aglutinante.…”

Section: Introductionunclassified

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Vieira¹,

Lupetti²,

Fatibello‐Filho³

2003

Quím. Nova

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were investigated as the source of peroxidase (POD: EC 1.11.1.7). Among those, zucchini (Cucurbita pepo) crude extract was found to be the best one. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of paracetamol to N-acetylp-benzoquinoneimine which the electrochemical reduction back to paracetamol was obtained at a peak potential of -0.10V. A cyclic voltammetric study was performed by scanning the potential from + 0.5 to -0.5 V. The recovery of paracetamol from two samples ranged from 97.3 to 106% and a rectilinear calibration curve for paracetamol concentration from 1.2x10 -4 to 2.5x10 -3 mol L -1 (r=0.9965) were obtained. The detection limit was 6.9x10 -5 mol L -1 and the relative standard deviation was less than 1.1% for a solution containing 2.5x10 -3 mol L -1 paracetamol and 2.0x10 -3 mol L -1 hydrogen peroxide (n=12). The results obtained for paracetamol in pharmaceutical products using the proposed biosensor and Pharmacopoeial procedures are in agreement at the 95% confidence level.

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Section: Introductionunclassified

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Vieira¹,

Lupetti²,

Fatibello‐Filho³

2003

Quím. Nova

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“…Similarly, covalent binding of the redox mediator via long and flexible spacer chains either to the electrode surface (seaweed mechanism) or to the outer surface of the enzyme itself (whip mechanism), has also been proposed as alternative [12,73]. Mixing the mediator into the carbon paste (graphite powder) is a relatively easy and effective method of mediator integration [74][75][76][77][78][79][80][81]. These carbon pastes can be further modified with stabilizers [82] or polyelectrolytes [75] to increase the long-term stability, response time, etc.…”

Section: Mediated Electron Transfer (Met)mentioning

confidence: 99%

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

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Recent interest in the field of biocommodities production through bioelectrochemical systems has generated interest in the enzyme catalyzed redox reactions. Enzyme catalyzed electrodes are well established as sensors and power generators. However, a paradigm shift in recent science towards the production of useful chemicals has changed the face of biofuel cells, keeping the fuels or chemicals production in the upfront. This review article comprehensively presents the progress in the field of enzyme-electrodes for the production of electricity, fuels and chemicals with an aim to represent a practical outline for understanding the use of single or multiple redox enzymes as electrocatalysts for their electron transfer onto electrodes. It also provides the state-of-the-art information regarding the different existing processes to fabricate enzyme electrodes. Successfully-achieved electroenzymatic anodic and cathodic reactions are further discussed, together with their potential applications. Particular focus was made on the novel single/multiple enzyme systems towards product synthesis and other applications. Finally, techno-economic and environmental elements for industrial processing with enzyme catalyzed bioelectrochemical system (e-BES) are anticipated, in order to provide useful strategies for further development of this technology.

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“…Since the first reported work on carbon paste enzyme electrodes (Matuszewski and Trojanowicz, 1988), a type of conductive matrix enzyme electrode, a significant amount of research has been focused on constructing enzyme electrodes by this approach (Gorton, 1995). For example, by immobilizing an oxidoreductase together with an electron transfer mediator on a carbon particle matrix and applying such a mixture onto a conductive electrode, an active membrane is formed where the concentration of a specific chemical reagent can be measured by amperometric means (Rosen-Margalit et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Model‐based optimization of a conductive matrix enzyme electrode

Detzel

Wie

et al. 2004

Biotech & Bioengineering

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A mathematical model has been developed to describe the mechanism for internal mass transfer and enzyme reaction kinetics of an amperometric conductive matrix enzyme electrode. The model is simplified and solved analytically to arrive at a representation for the response slope in the linear range as well as for the response time. This is the first time that the response time of an enzyme electrode is described by a mathematical model. Simulations give information on how the design parameters influence the performance of the electrode for a glucose oxidase catalyzed sensing reaction process. Based on this information, several designs were constructed and tested showing suitable agreement with theoretical predictions. Finally, an optimized electrode was designed and validated.

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Graphite paste-based enzymatic glucose electrode for flow injection analysis

Cited by 74 publications

References 0 publications

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Model‐based optimization of a conductive matrix enzyme electrode

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