Green biosynthesis of silver nanoparticles by Aspergillus niger and its antiamoebic effect against Allovahlkampfia spelaea trophozoite and cyst

Farrag, Haiam Mohamed Mahmoud; Mostafa, Fatma Abdel Aziz Mohamed; Mohamed, Mai; Huseein, Enas Abdelhameed Mahmoud

doi:10.1016/j.exppara.2020.108031

Cited by 19 publications

(10 citation statements)

References 47 publications

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“…When, M. mao wings extract (1 mL) was added to AgNO 3 solution (1 mM), rapid conversion to brown color observed within 30 minutes, indicated active MMAgNPs synthesis ( Fig 2B). The increased color change of brown color was directly proportional to the incubation period which is due to reduction of AgNO 3 and excitation of SPR [19]. The absorption peak varied with different pH and it ranged between 390-470 nm ( Fig 2C).…”

Section: Optimization Of Mmagnps Synthesismentioning

confidence: 90%

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Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential

et al. 2021

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Silver nanoparticles (AgNPs) are among the most widely synthesized and used nanoparticles (NPs). AgNPs have been traditionally synthesized from plant extracts, cobwebs, microorganisms, etc. However, their synthesis from wing extracts of common insect; Mang mao which is abundantly available in most of the Asian countries has not been explored yet. We report the synthesis of AgNPs from M. mao wings extract and its antioxidant and antimicrobial activity. The synthesized AgNPs were spherical, 40–60 nm in size and revealed strong absorption plasmon band around at 430 nm. Highly crystalline nature of these particles as determined by Energy-dispersive X-ray analysis and X-ray diffraction further confirmed the presence of AgNPs. Hydrodynamic size and zeta potential of AgNPs were observed to be 43.9 nm and -7.12 mV, respectively. Fourier-transform infrared spectroscopy analysis revealed the presence of characteristic amide proteins and aromatic functional groups. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis revealed the presence of fatty acids in the wings extract that may be responsible for biosynthesis and stabilization of AgNPs. Further, SDS-PAGE of the insect wing extract protein showed the molecular weight of 49 kDa. M. mao silver nanoparticles (MMAgNPs) exhibit strong antioxidant, broad-range antibacterial and antifungal activities, (66.8 to 87.0%), broad-range antibacterial and antifungal activities was found with maximum zone of inhibition against Staphylococcus aureus MTCC 96 (35±0.4 mm) and Fusarium oxysporum f. sp. ricini (86.6±0.4) which signifies their biomedical and agricultural potential.

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Section: Optimization Of Mmagnps Synthesismentioning

confidence: 90%

“…The change in color of reaction mixture from yellow to dark brown after 30 PLOS ONE minutes incubation (Fig 1) indicated synthesis of MMAgNPs. The intensity of dark brown colour change indicated AgNPs formation from silver salt which is due to excitation of surface plasmon resonance (SPR) effect [19,20].…”

Section: Synthesis Of Mmagnpsmentioning

confidence: 99%

Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential

et al. 2021

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“…Fungi have advantages over other microorganisms for the biosynthesis of nanoparticles due to high tolerance toward metals and high wall binding capacity as well as intracellular metal uptake capabilities; their high secretion of proteins, enzymes, and metabolites; high growth rates; easy handling in large-scale production; and low-cost requirements for production procedures. In addition, nanoparticles synthesized using fungi present fair mono-dispersity and stability compared to other microorganisms [ 32 ]. The exact mechanism for the production of NPs is still partly unclear, but the existence of bio-molecules in the biomass may be used for the production of NPs.…”

Section: Introductionmentioning

confidence: 99%

Application of Response Surface Methodology for Optimizing the Therapeutic Activity of ZnO Nanoparticles Biosynthesized from Aspergillus niger

et al. 2021

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In this study, the biosynthesis of zinc oxide nanoparticles using Aspergillus niger (A/ZnO-NPs) is described. These particles have been characterized by UV–Vis spectrum analysis, x-ray powder diffraction, field emission scanning electron microscopy, and transmission electron microscopy. To use this biosynthesized nanoparticle as an antiproliferative and antimicrobial agent, the IC50 value against the breast cancer cell line and inhibition zone against Escherichia coli were used to optimize the effect of two processing factors including dose of filtrate fungi cell and temperature. The biosynthesized A/ZnO-NPs had an absorbance band at 320 nm and spherical shapes. The mean particles size was 35 nm. RSM (response surface methodology) was utilized to investigate the outcome responses. The Model F-value of 12.21 and 7.29 implies that the model was significant for both responses. The contour plot against inhibition zone for temperature and dose showed that if the dose increases from 3.8 to 17.2 µg/mL, the inhibition zone increases up to 35 mm. As an alternative to chemical and/or physical methods, biosynthesizing zinc oxide NPs through fungi extracts can serve as a more facile and eco-friendly strategy. Additionally, for optimization of the processes, the outcome responses in the biomedical available test can be used in the synthesis of ZnO-NPs that are utilized for large-scale production in various medical applications.

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“…The numerous NMs have been synthesized by Aspergillus spp. and applied in various fields, for instance, Ag, Cu, Fe, Fe 3 O 4 , and ZnO NMs are some of them [55,56,72,[89][90][91][92][93][94][95]. Nanotechnology platform finds application of NMs in almost in each and every field such as agriculture, environmental science, health sciences, portable water treatment large/small scale plants, industrial separation, catalyst, electronics, energy storage, and energy regeneration [96][97][98][99].…”

Section: Applications Of Nanomaterials Synthesized Using Aspergillus Sppmentioning

confidence: 99%

“…The antibacterial and antifungal potential improves the health of the livestock. In another study, Farrag et al, [92] reported the synthesis of Ag NPs by Aspergillus niger isolated from soil by treatment with silver nitrate. AgNPs exhibited significant inhibition of Allovahlkampfia spelaea viability and growth of both trophozoites and cysts, with a reduction of amoebic cytotoxic activity in host cells that suggested the Ag NPs possibly will give a promising future for the treatment of Allovahlkampfia spelaea infections in humans.…”

Section: Applications In Healthcare Medicine and Pharmacymentioning

confidence: 99%

Industrial Applications of Nanomaterials Produced from Aspergillus Species

Rai¹,

Gupta²,

Bonde³

et al. 2022

The Genus Aspergillus - Pathogenicity, Mycotoxin Production and Industrial Applications

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There is a great demand for green methods of synthesis of nanoparticles. Fungi play an important role in the synthesis of nanoparticles, of which Aspergillus spp. are known to secrete different enzymes responsible for the synthesis of nanoparticles. The process of biosynthesis of nanoparticles is simple, rapid, cost-effective, eco-friendly, and easy to synthesize at ambient temperature and pressure. Mostly, the metal nanoparticles such as silver, gold, lead and the oxides of titanium, zinc, and copper are synthesized from Aspergillus spp. These include mainly Aspergillus fumigatus, A. flavus, A. niger, A. terreus, and A. clavatus. The fabrication of different nanoparticles is extracellular. In the present chapter, we have discussed the role of different species of Aspergillus, mechanism of biogenic synthesis particularly enzymes involved in the reduction of metal ions into nanoparticles. The biogenically synthesized nanoparticles have demonstrated several biomedicals, agricultural, and engineering applications. The biogenic nanoparticles are mostly used as antimicrobial and cytotoxic agents. Their use as fungicidal agents is important for sustainable agriculture.

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Green biosynthesis of silver nanoparticles by Aspergillus niger and its antiamoebic effect against Allovahlkampfia spelaea trophozoite and cyst

Cited by 19 publications

References 47 publications

Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential

Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential

Application of Response Surface Methodology for Optimizing the Therapeutic Activity of ZnO Nanoparticles Biosynthesized from Aspergillus niger

Industrial Applications of Nanomaterials Produced from Aspergillus Species

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