Green preparation of seaweed‐based silver nano‐liquid for cotton pathogenic fungi management

Abstract

Silver nanoparticles (Ag NPs) were synthesised using the crude ethyl acetate extracts of Ulva lactuca and evaluated their bioefficacy against two crop-damaging pathogens. The sets of lattice planes in the XRD spectrum for the Ag NPs were indexed to the 111, 200, 220 and 311 orientations and support the crystalline nature of the Ag NPs. The 3414 and 2968 cm −1 peaks were observed in crude algal thallus extract and they were characteristic of terpenoids. Further, a peak at 1389 cm −1 was observed as fatty acids. The marine macroalgae terpenoids and palmitic acid acted as reducing agent and stabiliser, respectively. The size (3 and 50 nm) and shape (spherical) of Ag NPs were recorded. The energy-dispersive X-ray spectroscopy analysis exemplified the presence of silver in its elemental nature. Moreover, U. lactuca Ag NPs were effective against two cotton phytopathogens namely Fusarium oxysporum f.sp. vasinfectum (FOV) and Xanthomonas campestris pv. malvacearum (XAM). The minimum inhibitory concentration was found to be 80.0 and 43.33 μg ml −1 against FOV and XAM, respectively. Results confirmed the anti-microbial activity of green nanoparticles against select pathogens and suggest their possible usage in developing antifungal agents for controlling destructive pathogens in a cotton agroecosystem.

Algal production of nano-silver and gold: Their antimicrobial and cytotoxic activities: A review

“…vasinfectum and Xanthomonas campestri s pv malvacearum that are responsible for significant yield losses in cotton worldwide. The biosynthesized Ag-NPs using P. pavonica inhibited the growth of the test pathogens as reported by Sahayaraj et al [116].…”

“…Sahayaraj et al [116], use marine algae Padina pavonica (Linn.) thallus broth in the extra cellular synthesis of biogenic Ag-NPs.…”

Profil fitokimia selada laut (Ulva lactuca) dan mikro alga filamen (Spirogyra sp) sebagai bahan alam bahari potensial dari perairan Indonesia

“…(Liu et al, 2020). Penelitian (Sahayaraj et al, 2019) menunjukkan bahwa terpenoid terindetifikasi pada selada laut.…”

“…Hal lain yang menarik adalah keberadaan spot dibawah sinar UV 365nm berwarna merah muda pada sistem fase gerak nonpolar n-heksana:etil asetat (9:1) dengan nilai Rf = 0,67 yang memberikan reaksi positif peredaman radikal bebas DPPH. Berdasarkan penelitian (Sahayaraj et al, 2019) selada laut (Ulva lactuca) dari pesisir pulau Thoothukudi Har, Tamil Nadu, India memiliki kandungan terpenoid dan asam palmitat yang berperan sebagai agen pereduksi. Terpenoid merupakan metabolit sekunder yang bersifat non polar dan banyak ditemukan pada ekstrak dengan pelarut non polar (n-heksan, benzen).…”

Synthesis of flexirubin-mediated silver nanoparticles using Chryseobacterium artocarpi CECT 8497 and investigation of its anticancer activity

“…Currently, a growing need exists to develop environmentally-benign processes for nanoparticle synthesis that do not employ toxic chemicals [10]. Incidentally, the advancement of 'green' chemistry to effectively synthesise silver nanoparticles using compounds derived from microbes has drawn remarkable attention in recent years [11].…”