Green synthesis and characterization of silver nanoparticles using aqueous petal extract of the medicinal plantCombretum indicum

Bahuguna, Gaurav; Kumar, Amit; Mishra, Neeraj Kumar; Kumar, Chanchal; Bahlwal, Aseema; Chaudhary, Pratibha; Singh, R. P.

doi:10.1088/2053-1591/3/7/075003

Cited by 19 publications

(13 citation statements)

References 10 publications

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“…UV-Vis spectroscopy was used to further examine the sample at various time intervals. The samples had valleys within 300-400 nm wavelength and peaks at 400-500 nm wavelength; the maximum wavelength from the combined graph see Figure 8 above was 430 nm which is characteristic of SNPs [15]. The curve in Figure 8 showed that with an increase in incubation time there was an increase in absorbance which was similar to previous reports.…”

Section: Discussionsupporting

confidence: 86%

Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity

Edith¹,

Uzor²

2021

ABB

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Nanotechnology is a fast-growing field of science. Nanoparticles get much attention due to their unique physicochemical, optical and thermal activities. Silver nanoparticles have been used in experiments to treat infectious diseases. The goal of the research was to make silver nanoparticles using Euphorbia hirta extract, physically characterize the nanoparticles obtained and, to evaluate silver nanoparticles' antibacterial properties. The leaf extract of E. hirta (asthma weed) was used for the reduction of 1 mM silver nitrate (AgNO 3 ) solution to silver nanoparticles (SNPs). SNPs were made by combining 50 mL of aqueous plant extract with 250 mL of AgNO 3 solution to make SNPs. The mixture was monitored for two hours. The synthesized SNPs were characterized by UV-Vis, FTIR spectroscopy and particle size. The antimicrobial activity of the SNPs was tested against Escherichia. coli, Pseudomonas. aeruginosa, Salmonella. typhi, Bacillus. subtilis and Candida. albicans. The reaction medium's hue shifted from yellow to brown. The results of the UV-vis analysis of the particles showed that at 430 nm the particles had the maximum absorption (λ max) within 2 hours. The FTIR identified carboxylic acid and other functional groups. The polydispersity index (PDI) and Z-average particle size were found to be 0.426 and 274 nm, respectively. The results of the antimicrobial studies showed sufficient growth inhibition of the bacteria by the SNPs the minimum inhibitory concentration (MIC) ranging from 7 µg-10 µg. It was concluded that SNPs were synthesized using E. hirta leaf extract. The synthesized SNPs possess good activity against pathogenic microorganisms.

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Section: Discussionsupporting

confidence: 86%

Green Synthesis of Silver Nanoparticles Using Euphorbia hirta Leaf Extract and the Determination of Their Antimicrobial Activity

Edith¹,

Uzor²

2021

ABB

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“…The wavelength scale was fixed between 300 and 600 nm, and the solution was scanned in this range. Maximum absorbance at 440 nm was observed, which is characteristic of silver nanoparticles (Bahuguna et al 2016). The curve (Fig.…”

Section: Resultsmentioning

confidence: 91%

Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms

2019

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Green synthesis of silver nanoparticles makes use of plant constituents, like carbohydrates, fats, enzymes, flavonoids, terpenoids, polyphenols, and alkaloids, as reducing agents to synthesize silver nanoparticles. The present study for the first time utilized seed extract of Tectona grandis (teak) for reduction of 1 mM silver nitrate solution to silver nanoparticles. The method proved to be very simple, cost-efficient, and convenient. Synthesis of nanoparticles was confirmed by visual detection in which the colorless solution gets changed to a brown-colored solution. Further characterization was done by UV-visible spectroscopy, XRD, FTIR analysis, SEM/EDS, FESEM, and TEM. Size of silver nanoparticles was found to be 10-30 nm approximately as determined by transmission electron microscopy (TEM). Energy-dispersive spectra (EDS) revealed that nanoparticles contain silver in its pure form. Well diffusion method showed the antimicrobial effect of AgNPs on different microorganisms with the zone of inhibition of 16 mm for Staphylococcus aureus, 12 mm for Bacillus cereus, and 17 mm for E. coli when 50 μg of AgNPs was used. Minimum inhibitory concentration was found to be 5.2, 2.6, and 2.0 μg/ml for Bacillus cereus, Staphylococcus aureus, and E. coli respectively. Mode of action of antimicrobial activity of nanoparticles was investigated by determining leakage of reducing sugars and proteins, suggesting that AgNPs were able to destroy membrane permeability.

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“…It is a well-recognised fact that the excitation of SPR in metal NPs causes the reaction mixture to change its colour to brown [ 52 ]. The collective oscillations of free electrons present in NPs, along with the light waves, give rise to a characteristic peak between 410 and 500 nm [ 53 , 54 ]. Very close to the findings of this study, an LSPR peak at 430 nm was displayed by the AgNPs synthesised from the pomace of black currant [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Sunlight-Mediated Green Synthesis of Silver Nanoparticles Using the Berries of Ribes rubrum (Red Currants): Characterisation and Evaluation of Their Antifungal and Antibacterial Activities

et al. 2022

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Plants are a treasure trove of several important phytochemicals that are endowed with therapeutic and medicinal properties. Ribes rubrum L. (red currants) are seasonal berries that are widely consumed for their nutritional value and are known for their health benefits. Red currants are a rich source of secondary metabolites such as polyphenols, tocopherols, phenolic acids, ascorbic acid, and flavonoids. In this study, sunlight-mediated synthesis of silver nanoparticles (AgNPs) was successfully accomplished within 9 min after adding the silver nitrate solution to the aqueous extract of red currant. The synthesised AgNPs were characterised with UV–Vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectrum (FTIR), and energy-dispersive X-ray spectrum (EDX). The efficacy of aqueous extracts of red currants and AgNPs in controlling the growth of some pathogenic fungi and bacteria was also investigated. The UV–visible (UV–Vis) spectrum displayed an absorption peak at 435 nm, which corresponded to the surface plasmon band. The strong silver signal on the EDX spectrum at 3 keV, authenticated the formation of AgNPs. The several peaks on the FTIR spectrum of the aqueous extract of red currant and the nanoparticles indicated the presence of some important functional groups such as amines, carbonyl compounds, and phenols that are vital in facilitating the process of capping and bioreduction, besides conferring stability to nanoparticles. The TEM microphotographs showed that the nanoparticles were well dispersed, roughly spherical, and the size of the nanoparticles ranged from 8 to 59 nm. The red currant silver nanoparticles were highly potent in inhibiting the growth and proliferation of some fungal and bacterial test isolates, especially Alternaria alternata, Colletotrichum musae, and Trichoderma harzianum. Based on the robust antifungal and antibacterial activity demonstrated in this study, red currant nanoparticles can be investigated as potential replacements for synthetic fungicides and antibiotics.

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Green synthesis and characterization of silver nanoparticles using aqueous petal extract of the medicinal plantCombretum indicum

Cited by 19 publications

References 10 publications

Green Synthesis of Silver Nanoparticles Using <i>Euphorbia hirta</i> Leaf Extract and the Determination of Their Antimicrobial Activity

Green Synthesis of Silver Nanoparticles Using <i>Euphorbia hirta</i> Leaf Extract and the Determination of Their Antimicrobial Activity

Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms

Sunlight-Mediated Green Synthesis of Silver Nanoparticles Using the Berries of Ribes rubrum (Red Currants): Characterisation and Evaluation of Their Antifungal and Antibacterial Activities

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Green synthesis and characterization of silver nanoparticles using aqueous petal extract of the medicinal plantCombretum indicum

Cited by 19 publications

References 10 publications

Green Synthesis of Silver Nanoparticles Using &lt;i&gt;Euphorbia hirta&lt;/i&gt; Leaf Extract and the Determination of Their Antimicrobial Activity

Green Synthesis of Silver Nanoparticles Using &lt;i&gt;Euphorbia hirta&lt;/i&gt; Leaf Extract and the Determination of Their Antimicrobial Activity

Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms

Sunlight-Mediated Green Synthesis of Silver Nanoparticles Using the Berries of Ribes rubrum (Red Currants): Characterisation and Evaluation of Their Antifungal and Antibacterial Activities

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Green Synthesis of Silver Nanoparticles Using <i>Euphorbia hirta</i> Leaf Extract and the Determination of Their Antimicrobial Activity

Green Synthesis of Silver Nanoparticles Using <i>Euphorbia hirta</i> Leaf Extract and the Determination of Their Antimicrobial Activity