In this study, we used the aqueous extract of garlic tunicate leaf to reduce a mixture of equal amounts of ruthenium chloride, silver nitrate, and palladium acetate for the biosynthesis of ruthenium/silver/palladium trimetallic nanocomposite (Ru/Ag/Pd)-Np. Some physicochemical tools were used for nanocomposite characterization, including Fourier-transform infrared spectroscopy (FT-IR), X-ray di raction (XRD), thermal gravimetric analysis (TGA), UV-Vis spectroscopy (UV-Vis), scanning electron microscope (SEM), and transmittance electron microscope (TEM). XRD revealed that the crystal size of the nanocomposite is 15.67 nm. e TEM images showed that the particle size ranged 50-90 nm. e antimicrobial e cacy of the nanocomposite was examined against Aspergillus avus, Aspergillus niger, Candida albicans, Candida glabrata, Escherichia coli, and Bacillus cereus.e results showed a potent antimicrobial activity toward all tested microorganisms. (Ru/Ag/Pd)-Np showed antiproliferative activity against Caco-2, HepG2, and K562 cell lines. e antiproliferative potential of (Ru/Ag/Pd)-Np was signi cantly improved following UV irradiation.