Green synthesis of nanoparticles with extracellular and intracellular extracts of basidiomycetes

Abstract

Au, Ag, Se, and Si nanoparticles were synthesized from aqueous solutions of HAuCl4, AgNO3, Na2SeO3, and Na2SiO3 with extra- and intracellular extracts from the xylotrophic basidiomycetes Pleurotus ostreatus, Lentinus edodes, Ganoderma lucidum, and Grifola frondosa. The shape, size, and aggregation properties of the nanoparticles depended both on the fungal species and on the extract type. The bioreduction of the metal-containing compounds and the formation rate of Au and Ag nanoparticles depended directly on the phenol oxidase activity of the fungal extracts used. The biofabrication of Se and Si nanoparticles did not depend on phenol oxidase activity. When we used mycelial extracts from different fungal morphological structures, we succeeded in obtaining nanoparticles of differing shapes and sizes. The cytotoxicity of the noble metal nanoparticles, which are widely used in biomedicine, was evaluated on the HeLa and Vero cell lines. The cytotoxicity of the Au nanoparticles was negligible in a broad concentration range (1–100 µg/mL), whereas the Ag nanoparticles were nontoxic only when used between 1 and 10 µg/mL.

Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics

“…Recently various types of metal NPs have been synthesized biologically by reducing and capping of metal ions using extracts of macrofungi such as Pleurotus ostreatus, Lentinus edodes, Ganoderma lucidum, and Grifola frondosa [ 4 , 5 ]. Vigneshwaran et al [ 6 ] and Gade et al [ 7 ] have synthesized silver-protein NPs and silver NPs, respectively, using P. sajor-caju extracts.…”

Anti-bacterial/fungal and anti-cancer performance of green synthesized Ag nanoparticles using summer savory extract

“…AgNPs, due to their unique properties, have been remarkably effective in various fields of science, including biology, biomedicine, chemical engineering, environment, and many other fields of science. It also plays a significant role in nanotechnology and nanoscience, particularly in nanomedicine [7][8][9]. Silver NPs have antimicrobial, antitumor, antioxidant, and anti-inflammatory properties, which have provided extensive open possibilities for the usage of these nanoparticles in biomedicine.…”

“…Silver NPs have antimicrobial, antitumor, antioxidant, and anti-inflammatory properties, which have provided extensive open possibilities for the usage of these nanoparticles in biomedicine. [7][8][9][10]. A schematic diagram representing various applications of AgNPs has been provided in Figure 1.…”

Biological Synthesis of Nanoparticles Using Endophytic Microorganisms: Current Development

“…After incubation, the culture is centrifuged to separate the biomass from the supernatant [48]. Both supernanant and biomass are tested for nanoparticles synthesize, in fact microorganisms are able to synthesize nanoparticles extracellularly or intracellularly (Figure 3) [77].…”