“…Hence our results justify the plausible mechanisms (Fig. 14 ) for potent microbicidal activity of Pa-AgNPs that were in accordance with the reported literature [ 60 – 62 ] of AgNPs synthesized by biogeneic approach. Fig.…”
Endophyte mediated nanoparticles fabrication were emerging as a new frontier in nanomedicines that produce high biocompatible and functionalized silver nanoparticles. In this study, silver nanoparticles were successfully biosynthesized from the extracellular extract of endophytic bacterium
Pantoea anthophila
isolated from the stem of
Waltheria indica
for the first time. The synthesized nanoparticles showed a strong absorption band at 410 nm in the UV–Visible range. The dynamic light scattering and zeta potential analysis indicated that the average particle size was 16 nm at 5.30 mV. FTIR spectrum displayed the presence of various functional groups at 3423.65, 1633.71, 1022.27, 607.58 cm
−1
that stabilised the nanoparticle. X-ray diffraction peaks were conferred to 100, 200, 220 and 311 planes of a face centred cubic structure. TEM and SEM micrograph revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analysed by EDAX. Selected area electron diffraction also confirms the orientation of silver nanoparticles with X-ray diffraction analysis. Antimicrobial activity against 10 different human pathogenic bacteria and fungi showed a broad spectrum inhibition against both Gram-positive and Gram-negative bacteria. Among the bacterial pathogens,
B. Subtilis
exhibited low activity compared to other pathogens.
C. albicans
was greatly controlled than other fungal species. A strong free radical scavenging activity of silver nanoparticles with IC
50
values 31.29 ± 0.73, 19.83 ± 1.57, 35.64 ± 0.94, 42.07 ± 1.30, 29.70 ± 2.26, 29.10 ± 0.82, 36.80 ± 0.63 μg/ml was obtained in different antioxidant assays that were comparable to the reference. The study suggests that the silver nanoparticles can be biosynthesized from endophytic
P. anthophila
metabolites with significant therapeutic potential. With proper validation, the biosynthesized silver nanoparticles can be developed as a promising antiviral and anticancer drug candidate.
“…Hence our results justify the plausible mechanisms (Fig. 14 ) for potent microbicidal activity of Pa-AgNPs that were in accordance with the reported literature [ 60 – 62 ] of AgNPs synthesized by biogeneic approach. Fig.…”
Endophyte mediated nanoparticles fabrication were emerging as a new frontier in nanomedicines that produce high biocompatible and functionalized silver nanoparticles. In this study, silver nanoparticles were successfully biosynthesized from the extracellular extract of endophytic bacterium
Pantoea anthophila
isolated from the stem of
Waltheria indica
for the first time. The synthesized nanoparticles showed a strong absorption band at 410 nm in the UV–Visible range. The dynamic light scattering and zeta potential analysis indicated that the average particle size was 16 nm at 5.30 mV. FTIR spectrum displayed the presence of various functional groups at 3423.65, 1633.71, 1022.27, 607.58 cm
−1
that stabilised the nanoparticle. X-ray diffraction peaks were conferred to 100, 200, 220 and 311 planes of a face centred cubic structure. TEM and SEM micrograph revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analysed by EDAX. Selected area electron diffraction also confirms the orientation of silver nanoparticles with X-ray diffraction analysis. Antimicrobial activity against 10 different human pathogenic bacteria and fungi showed a broad spectrum inhibition against both Gram-positive and Gram-negative bacteria. Among the bacterial pathogens,
B. Subtilis
exhibited low activity compared to other pathogens.
C. albicans
was greatly controlled than other fungal species. A strong free radical scavenging activity of silver nanoparticles with IC
50
values 31.29 ± 0.73, 19.83 ± 1.57, 35.64 ± 0.94, 42.07 ± 1.30, 29.70 ± 2.26, 29.10 ± 0.82, 36.80 ± 0.63 μg/ml was obtained in different antioxidant assays that were comparable to the reference. The study suggests that the silver nanoparticles can be biosynthesized from endophytic
P. anthophila
metabolites with significant therapeutic potential. With proper validation, the biosynthesized silver nanoparticles can be developed as a promising antiviral and anticancer drug candidate.
“…The antimicrobial effect of Pa-AgNPs against C. albicans was strong compared to the crude W.indica leaf extracts reported by Koma et al [17]. The results obtained justi es potent microbicidal activity of Pa-AgNPs that were also relevant with the reported literatures [45][46][47] of AgNPs. Presence of charge, size, shape, concentration, dissolution rate, species type, tolerance and time of exposure may attribute to the antimicrobial property of AgNPs [21,[48][49][50].…”
Section: Antimicrobial Screening Of Pa-agnpssupporting
Endophyte mediated nanoparticles fabrication was emerging as a new frontier in nanomedicines that produce high biocompatible and functionalized silver nanoparticles. In this study, silver nanoparticles were successfully biosynthesized from the extracellular extract of endophytic bacterium Pantoea anthophila isolated from the stem of Waltheria indica for the first time. The synthesised nanoparticles were characterized by UV-Visible and Fourier Transform Infra-Red spectroscopy. The structural analysis is done by X-ray diffraction and the stability was studied by dynamic light scattering and particle size analyser. The size and shape were observed by Transmission Electron Microscope, Scanning Electron Microscope and Energy Dispersive X-Ray spectrum. Further, the nanoparticles were evaluated for antimicrobial and antioxidant properties. Synthesized nanoparticle showed a strong absorption band in the UV-Visible range at 410 nm. The average particle size was found to be 16.8 nm with spherical shaped, crystalline nature. Good zones of inhibition at various ranges were detected against a broad range of human pathogenic bacteria and fungi. A strong free radical scavenging activity of silver nanoparticles with IC50 values 30.75, 19.47, 34.59, 41.12, 27.24, 28.16, 36.21 µg/ml was obtained that was comparable to the reference. The study suggests that the silver nanoparticles can be biosynthesised from endophytic P. anthophila metabolites with significant therapeutic potential. With proper validation, the biosynthesised silver nanoparticles can be developed as a promising antiviral and anticancer drug candidate.
“…Mishra et al, (49) reported similar results in the study done by them and revealed the existence of metallic silver in biosynthesized nanoparticles with a sharp peak at 3 keV. They also showed the occurrence of other peaks (Ca, Si, Mg, and Na) as were in our study.…”
Section: Proposed Antibacterial Mechanism Of Vw-agncs Against a Bauma...supporting
Background
Antimicrobial resistance is peaking up all over the world and requires immediate attention. In quest of finding a sustainable solution to the present threat of future epidemics which is going to be caused by Acinetobacter baumannii (A. baumannii) as per the WHO report (2020), this study has been used to pave a path for finding an herbal solution to it. The novelty of this study is that Valeriana wallichii (V. wallichii) has been analyzed for its methanolic extract/nano-conjugate against A baumannii.
Results
HPLC of the extract has shown the presence of polyphenols- Quercitin, Rutin, and Tannic acid in high concentration. This fraction on nano-conjugation with silver has shown its equivalence to tetracycline antibiotics in restricting the growth of A. baumannii. Further, insilico studies taking crucial death receptors (Amp C, Fab1, RstA, LpxA, Aspartokinase, and shikimate dehydrogenase) of A. baumannii have shown binding energy ranging from − 11.6 to -13.3 kcal/mol for Tannic acid and close to this for Rutin and Linalool. It signifies that the fraction is potent enough to be a potential drug candidate for restricting this bacterium. The results were amazing when V. wallichii silver nanoconjugate (Vw-AgNCs), size range 1-200nm validated by DLS, AFM, and SEM-EDX on green synthesis has many times substantially enhanced restriction of A. baumannii as compared to only extract of V. wallichii. Keeping in view, its application to mankind, the cytotoxicity of the extract was evaluated on the human Fibroblast cell line (L929), showing an LC50 value of 368ug/ml. Many types of research have shown the time-dependent cytotoxicity of antibiotics as low as 5-50ug/ml providing us with a huge therapeutic window for the Vw-AgNCs.
Conclusion
Vw-AgNCs exhibited strong antimicrobial activity than the only extract and chemically synthesized nanoparticles so they can be a potential antimicrobial agent against A. baumannii in the future.
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