Green Synthesis of Silver Nanoparticles Using Leaf Extracts of Clitoria ternatea and Solanum nigrum and Study of Its Antibacterial Effect against Common Nosocomial Pathogens

Abstract

Bionanotechnology has emerged up as integration between biotechnology and nanotechnology for developing biosynthetic and environmental friendly technology for synthesis of nanomaterials. Silver has been known to have effective bactericidal properties for centuries. Nowadays, silver based topical dressings have been widely used as a treatment for infection in burns, open wounds, and chronic ulcer. As the pathogenic organisms are getting evolved day by day due to mutation and gaining antibiotic resistance, an important industrial sector of nanoscience deals with the preparation and study of nanoparticles in antibacterial clothing, burn ointments, and coating for medical device. The size of nanomaterials is much smaller than that of most biological molecules and structures; therefore, nanomaterials can be useful in both in vivo and in vitro biomedical research application. The purpose of the study is to synthesize and characterize the plant mediated silver nanoparticles using Clitoria ternatea and Solanum nigrum. Further investigation of the shape and size of nanoparticle was done by X-ray diffraction and scanning electron microscopic studies. A silver nanoparticle at different concentration was assessed for its antibacterial effect, against various nosocomial pathogens.

Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review

“…Nowadays, production of NPs focuses on green synthesis from extract of different plant parts. The multipurpose agents of reduction and stabilization of plant extraction for biological synthesis of NPs use to implement green chemistry [52,53]. Extraction of nontoxic plants for synthesis nanoparticle offer natural capping agents.…”

Antimicrobial activity of biogenic silver nanoparticles, and silver chloride nanoparticles: an overview and comments

“…Probably, traces of Ag o nanoparticles and some AgCl nanoparticles were presented in the low-quality XRD diffractions pattern reported (Pasupuleti et al 2013). Clitoria ternatea and Solanum nigrum extracts were suggested to produce Ag o nanoparticles: however, from the XRD pattern, it is clear the presence of AgCl nanoparticles (Krithiga et al 2015). Therefore, inadequate XRD analysis occurs during characterization of biogenic synthesized silver-based nanoparticles.…”

Green Synthesis of Silver Nanoparticles Using Medicinal Plant Acalypha Indica Leaf Extracts and Its Application as an Antioxidant and Antimicrobial Agent Against Foodborne Pathogens

“…The plant or microbial mediated methods are widely being followed as they are simple, cost effective, eco-friendly and give rapid results. As, plants are rich in various proteins and secondary metabolites including quinones, flavonoids, saponins, and terpenoids, which are involved in the stabilization or reduction of the nanoparticles [1][2][3][4].…”