Green synthesis of silver nanoparticles for selective toxicity towards cancer cells

Govindaraju, K.; Karthikeyan, K.; Alsagaby, Suliman A.; Ganesan, Singaravelu; Premanathan, Mariappan

doi:10.1049/iet-nbt.2015.0001

Cited by 97 publications

(46 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with our results, several studies have shown that ZnO NPs or other types of NPs induce DNA damage in a variety of cell types. [50][51][52][53][54][55][56][57][58][59][60] In vivo exposure to ZnO NPs alters the structure of seminiferous tubules and spermatozoa morphologies…”

Section: Zno Nps Induce Nuclear Dna Leakage and Breakagementioning

confidence: 99%

Cytotoxic effects of ZnO nanoparticles on mouse testicular cells

Han¹,

Yan²,

Ge³

et al. 2016

IJN

102

View full text Add to dashboard Cite

Nanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model

show abstract

Section: Zno Nps Induce Nuclear Dna Leakage and Breakagementioning

confidence: 99%

Cytotoxic effects of ZnO nanoparticles on mouse testicular cells

Han¹,

Yan²,

Ge³

et al. 2016

IJN

102

View full text Add to dashboard Cite

show abstract

“…These results were similar to the effects of silver nanoparticles on the cancer cells of the HL60 myeloblastic leukemia, Hella's cancer cells and healthy peripheral blood cells. With the influences of silver nanoparticles on cells and peroxidation of fats, free radicals of ROS are formed that damage DNA and induce apoptosis (35). In addition, in the present study, apoptosis was evaluated in HPB-ALL cell line and lymphocytes by silver nanoparticles and Flow Cytometry that showed the values of 22.75% and 6.04%, respectively.…”

Section: Discussionmentioning

confidence: 75%

Comparative Study of the Cytotoxic Effect of Silver Nanoparticles on Human Lymphocytes and HPB-ALL Cell Line: As an In Vitro Study

Farahani¹,

Parivar²,

Roodbari³

et al. 2020

Iran Red Crescent Med J

View full text Add to dashboard Cite

Background: Cancer is currently the second leading cause of death worldwide that is originated from cell growth and proliferation without control. Acute lymphoblastic leukemia (ALL) is one of the types of leukemia that affects lymphocyte maturation and it is common among children. Silver nanoparticles are considered one of the targeted chemotherapy methods by creating cytotoxicity. Objectives: In this research, a comparative study of cytotoxic effect of silver nanoparticles was evaluated on human lymphocytes and HPB-ALL cell line as an in vitro study. Methods: In this experimental study, lymphocytes and HPB-ALL cell line were exposed to silver nanoparticles at RPMI 1640 medium culture in order to assess toxicity for 24 hours. To this aim, MTT assay was used to evaluate the toxicity of the silver nanoparticles. DNA fragmentation and apoptosis were evaluated by Gel Electrophoresis and Flow Cytometry, respectively. Moreover, quantitative PCR was performed on bax, bcl-2, and caspase-9 genes. Results:The results of MTT assay showed IC50 values of silver nanoparticles were 5.87 and 2.68 µg/mL for lymphocytes and HPB-ALL cell line, respectively. The results showed that silver nanoparticles could split DNA of the HPB-ALL cell line more than DNA of the lymphocytes during DNA fragmentation. Flow Cytometry results indicated that the early apoptosis was 6.04% and 22.75% in lymphocytes and HPB-ALL cell line, respectively. Moreover, Q-PCR results showed a significant up-regulation of caspase-9 and bax genes and downregulation of bcl-2 gene in comparison to the control group. Conclusions: The silver nanoparticles had cytotoxic effects on the lymphocytes and HPB-ALL cell line. The results showed that the silver nanoparticle had a significant cytotoxic effect on HPB-ALL cell line.

show abstract

“…Microscopy studies showed the endocytosis of silver nanoparticles into the nucleus of the cell which caused DNA damaged and ultimately led to apoptosis. These findings show potential for silver nanoparticles to be used in cancer therapy [151].…”

Section: Use Of Silver Nanoparticles For Drug Delivery and As Cancer mentioning

confidence: 70%

“…Because of silver nanoparticles extraordinary antimicrobial activity it is mainly used in the medical industry for textiles, wound dressings and device coatings. However, the use of silver nanoparticles in biomedical applications such as biosensors [133][134][135] photothermal therapy and drug delivery [144][145][146][147][148][149][150][151][152][153][154] is increasing and will be discussed in Section 4.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%