We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using
Withania somnifera
extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of
W. somnifera
. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO
3
were prepared and compared for their antimicrobial activity against human pathogens (
Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli
, and
Candida albicans
) and a plant pathogen (
Agrobacterium tumefaciens
). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.