Grouper Imotilization Technique Epinephelus sp. Using Datura Metel L. Seed Extract

Abstract

This study is about fish anesthesia using natural anesthetic ingredients. Natural anesthetic ingredients aim to reduce metabolism in Epinephelus sp. grouper fish. The immobilization method can be used to minimize high metabolism in Epinephelus sp. grouper fish. The purpose of this study was to obtain the influence of Datura metel L. seed extract on the onset time, recovery time, and live graduation of grouper fish Epinephelus sp. The results of the study, using extracts of natural anesthetic ingredients Datura metel L.seed with concentrations of 25%, 30%, and 35% can be concluded that it has a different influence (P<0.05) on the onset and recovered grouper fish. This study gave a life graduation rate of 100% in each concentration. The best concentration in this study was 35% concentration, with the fastest onset time of 3 minutes with a recovery time of 10.67 minutes. This study used a non-Factorial Complete Randomized Design

GC-MS Analysis of Various Crude Extracts from the Leaves, Flowers, and Stems of Datura metel Linnaeus 1753 and the Potential Activity as Anesthetic Agents on Fish

“…The advantages of natural anesthetics include low impact on the environment, no residue, cheaper, and effective at low concentrations compared to synthetic anesthetics (Purbosari et al, 2019). There are several herbal plants that have bioactive components as anesthetic agents that have been identified, where Datura metel (local name is kecubung) is one of them that has high potential as an anesthetic compound (Adebayo and Olufayo, 2017;Hariyanto et al, 2009;Palmi et al, 2019;Akbar et al, 2021;Saputra et al, 2021).…”

“…Studies on extraction solutions, chemical compositions of extracts from various parts of the plant genus Datura have been analyzed and their anesthetic activity tested on various types of fish such as Cyprinus carpio (Rahanandeh et al, 2022), Epinephelus sp. (Saputra et al, 2021), Heterobranchus bidorsalis (Adebayo and Olufayo, 2017), Oreochromis niloticus (Palmi et al, 2019), and Osphronemus gourami (Mashuda et al, 2020). In addition, the effectiveness of this plant anesthetic has also been tested on dogs (Bbalola et al, 2014) and rabbits (Elsa et al, 2001).…”