Growth and development of the organism and immunophysiological indices of blood of male F2 rats, affected by different doses of nanogermanium citrate

Abstract

Aim. To study age-related changes in the bodyweight, internal organs, and immunophysiological indices of blood for male F 2 rats, affected by different doses of nanogermanium citrate. Methods. Physiological, immunological, clinical, colorimetric, and statistical methods were applied; automatic veterinary analyzer Orphee Mythic 18 Vet (Germany) was used to determine hematological indices. Results. The decade-wise dynamics of changes in the bodyweight, the weight of heart, liver, kidneys, testicles, lungs, spleen, and weight coeffi cients of these organs at the age of 2–3 and 4–5 months demonstrated that there were intergroup dose-dependent and age-related differences in the intensity of growth and development of the organism of male rats. The highest gain of bodyweight was registered for male rates, which received 10 μg Ge/kg of bodyweight 30 days after weaning, this tendency was kept for the lower level of these indices in groups ІІІ and IV at the effect of 20 and 200 μg Ge. On days 97, 107, and 117, males of group II and IV demonstrated non-reliably lower indices of bodyweight, whereas in group III their values did not differ much from those for the control. Reliably higher indices of weight of liver and spleen were demonstrated along with their weight coeffi cients for younger males, but they were lower at the age of 4–5 months compared to the control. The blood of 2–3 m.o. group II males had reliably lower content of hemoglobin and hematocrit index as well as the tendency to the decrease in the number of erythrocytes. At the age of 4–5 months, there was an evident decrease in the number of leukocytes and their forms – lymphocytes, monocytes, and granulocytes, which was more expressed in the blood of group II and IV males compared to the control. At the impact of nanogermanium citrate (NGeC), the blood of males of experimental groups of both age periods demonstrated higher content of immunoglobulins, circulating immune complexes, hexoses, bound to proteins, and ceruloplasmin (except for group II animals at the age of 4 months). The effect of applied doses of NGeC inhibited the input of acute phase proteins into the blood which was evident in the reliably lower content of mean mass molecules at the age of 2–3 months with its staying at the level of the control group of animals at the age of 4– months. Conclusions. The biological effect of NGeC in the doses of 10, 20, and 200 μg Ge/kg of bodyweight was demonstrated in differently directed changes in the intensity of organism growth and development, specifi c internal organs, and hematological indices, which was more expressed for animals, receiving 10 μg Ge, as well as in the differences in immunophysiological indices of blood of males of the experimental groups of both age periods, which indicated the activation of immunobiological reactivity of their organism at the age of 2–3 and 4–5 months.

Main directions of research and achievements of the Laboratory of ecological physiology and product quality in 2000–2020

“…З огляду на актуальність дослідження з цього напряму, у 2011-2015 рр. поглиблено вивчення біологічної дії різних доз і поєднань «наноаквахелатів» Хрому, Кобальту, Марганцю, Міді, Заліза, Селену, Германію та Йоду в організмі, їхній вплив на імунобіологічну реактивність тварин, формування продуктивності і репродуктивної здатності [14,35]. Успішному виконанню цих досліджень сприяли молоді науковці, докторанти та аспіранти лабораторії під керівництвом докторів наук І. І. Ковальчук, Р. С. Федорука, Й. Ф. Рівіса (фото 4).…”

Impact of feeding male rats F2 with different doses of germanium citrate on the content of traceelements in their tissues and organs

“…The regulatory impact of physiologically active, but insuf¿ ciently studied elements, among which germanium takes a prominent place, on organtissue distribution of other minerals, is presently actively studied in biology, medicine, and veterinary science [3,4]. Organic formulations of macro-and trace elements (nanoaquachelates) as obtained by nanotechnology methods receive special attention [5,6]. The IMPACT OF FEEDING MALE RATS F 2 WITH DIFFERENT DOSES OF GERMANIUM CITRATE biological role of these organo-compounds and their interaction with other macro-and trace elements and their impact on their distribution in the organism and its organs are actively studied as well.…”

“…A notable characteristic of organic forms of Ge is that they are removed fast with urine from the organism, which indicates its low accumulation in tissues [4,10]. Different concentrations and duration of Ge intake affect physiological-biochemical processes in the organism differently, including their inÀ uence on the level of macro-and trace elements in tissues [3,6,7]. Recent experimental studies on physiological mechanisms of the effect of different doses of germanium citrate on organ-tissue and systemic level [6,7,9] and preparations, including Astrogerm, Germatranol, Germavit, elaborated on the basis of this chelate complex, stimulated a profound investigation of this compound on the intake of such vital elements as Cu, Co, Mn, Fe, Zn in the organism, the results of which are reported in this article.…”

Living in science and for science (in memory of Rostyslav Fedoruk)

“…Досліджено введення різних кількостей наноаквацитратів мікроелементів (Se, Cr, Co, Zn, Fe) до раціонів тварин, встановлено мінімальні фізіологічно активні та оптимальні їх дози для великої рогатої худоби та кролів. Досліджено вплив цих сполук на: формування імунобіологічної реактивності в організмі, вміст у тканинах і рідинах макро-і мікроелементів, стан антиоксидантної, дезінтоксикаційної, репродуктивної та імунної систем, їхній вплив на ріст і розвиток телят, кроленят, а також роль у лікуванні та профілактиці мікроелементозів у тварин [13,16,17,22,36].…”