Growth factors, as biological macromolecules in bioactivity enhancing of electrospun wound dressings for diabetic wound healing: A review

Zhang, Yunwu; Luo, Jingsong; Zhang, Qi; Deng, Tingting

doi:10.1016/j.ijbiomac.2021.09.210

Cited by 45 publications

(18 citation statements)

References 112 publications

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“…Another approach, involving the direct application of angiogenic growth factors to improve angiogenesis, has been tested in several clinical trials which have reported minimal success. These attempts have relied on the use of a single protein or combinations of up to three proteins, such as VEGF, PDGF, TGFβ, ANGPT1, and other growth factors, to improve overall wound repair (33,34,35). Interest has turned to gene therapy, since it may be possible to facilitate sustained production of angiogenic products by altering gene expression (36, 37, 38).…”

Section: Discussionmentioning

confidence: 99%

Transfection of Hypoxia-Inducible Factor-1α mRNA Upregulates the Expression of Genes Encoding Angiogenic Growth Factors

Włodarczyk

Leng

Abadchi

et al. 2023

Preprint

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Hypoxia-Inducible Factor-1α (HIF-1α) has presented a new direction for ischemic preconditioning of surgical flaps to promote their survival. In a previous study, we demonstrated the effectiveness of HIF-1a DNA plasmids in this application. In this study, to avoid complications associated with plasmid use, we sought to express HIF-1α through mRNA transfection and determine its biological activity by measuring the upregulation of downstream angiogenic genes. We transfected six different HIF-1a mRNAs–one predominant, three variant, and two novel mutant isoforms–into primary human dermal fibroblasts using Lipofectamine, and assessed mRNA levels using RT-qPCR. At all time points examined after transfection (3, 6, and 10 hours), the levels of HIF-1α transcript were significantly higher in all HIF-1α transfected cells relative to the control (all p < 0.05, unpaired Student’s T-test). Importantly, the expression of HIF-1α transcription response genes (VEGF, ANG-1, PGF, FLT1, and EDN1) was significantly higher in the cells transfected with all isoforms than with the control at six and/or ten hours post-transfection. All isoforms were transfected successfully into human fibroblast cells, resulting in the rapid upregulation of all five downstream angiogenic targets tested. These findings support the potential use of HIF-1α mRNA for protecting ischemic dermal flaps.

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Section: Discussionmentioning

confidence: 99%

Transfection of Hypoxia-Inducible Factor-1α mRNA Upregulates the Expression of Genes Encoding Angiogenic Growth Factors

Włodarczyk

Leng

Abadchi

et al. 2023

Preprint

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“…Nevertheless, in diabetic wounds, the overexpression of inflammatory cytokines causes the degradation of a significant amount of growth factors and prevents the complete healing of diabetic wounds. Therefore, the necessity of introducing growth factors in the therapy of diabetic wounds has increased considerably [ 167 ]. In addition, growth factors suffer from short half-life and poor stability, while the large surface area and high porosity structure of electrospun fibers could serve as an effective delivery system for growth factors.…”

Section: Electrospinning Nanofiber In Diabetic Wound Therapymentioning

confidence: 99%

Advances in the Preparation of Nanofiber Dressings by Electrospinning for Promoting Diabetic Wound Healing

Liu

Feng

et al. 2022

Biomolecules

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Chronic diabetic wounds are one of the main complications of diabetes, manifested by persistent inflammation, decreased epithelialization motility, and impaired wound healing. This will not only lead to the repeated hospitalization of patients, but also bear expensive hospitalization costs. In severe cases, it can lead to amputation, sepsis or death. Electrospun nanofibers membranes have the characteristics of high porosity, high specific surface area, and easy functionalization of structure, so they can be used as a safe and effective platform in the treatment of diabetic wounds and have great application potential. This article briefly reviewed the pathogenesis of chronic diabetic wounds and the types of dressings commonly used, and then reviewed the development of electrospinning technology in recent years and the advantages of electrospun nanofibers in the treatment of diabetic wounds. Finally, the reports of different types of nanofiber dressings on diabetic wounds are summarized, and the method of using multi-drug combination therapy in diabetic wounds is emphasized, which provides new ideas for the effective treatment of diabetic wounds.

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“…Among these therapeutic regimens, wound dressing is applied widely in the clinic owing to the isolation from an external environment and the delivery of medicine to wound sites directly . Multiple kinds of wound dressings have been developed for accelerating diabetic wound healing, like biocompatible membranes, electrospun fibers, and functional hydrogels . Among the candidates, hydrogels have attracted much more attention due to the unique features including biocompatibility, porous structure, absorption of excess tissue exudate, and facilitation of therapeutic factor delivery. , …”

Section: Introductionmentioning

confidence: 99%

Fabrication of an Adhesive Small Intestinal Submucosa Acellular Matrix Hydrogel for Accelerating Diabetic Wound Healing

Chen,

Hu,

et al. 2023

ACS Omega

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The treatment of diabetic skin defects comes with enormous challenges in the clinic due to the disordered metabolic microenvironment. In this study, we therefore designed a novel composite hydrogel (SISAM@HN) with bioactive factors and tissue adhesive properties for accelerating chronic diabetic wound healing. Hyaluronic acid (HA) modified by N -(2-aminoethyl)-4-(4-(hydroxymethyl)-2-methoxy-5-nitrosophenoxy) butanamide (NB) held the phototriggering tissue adhesive capacity. Decellularized small intestinal submucosa (SIS) was degreased and digested to form the acellular matrix, which facilitated bioactive factor release. The results of the burst pressure test demonstrated that the in situ formed hydrogel possessed a tissue adhesive property. In vitro experiments, based on bone marrow stromal cells, revealed that the SIS acellular matrix-containing hydrogel contributed to promoting cell proliferation. In vivo , a diabetic mouse model was created and used to evaluate the tissue regeneration function of the obtained hydrogel, and our results showed that the synthesized hydrogel could assist collagen deposition, attenuate inflammation, and foster vascular growth during the wound healing process. Overall, the SIS acellular matrix-containing HA hydrogel was able to adhere to the wound sites, promote cell proliferation, and facilitate angiogenesis, which would be a promising biomaterial for wound dressing in clinical therapy of diabetic skin defects.

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Growth factors, as biological macromolecules in bioactivity enhancing of electrospun wound dressings for diabetic wound healing: A review

Cited by 45 publications

References 112 publications

Transfection of Hypoxia-Inducible Factor-1α mRNA Upregulates the Expression of Genes Encoding Angiogenic Growth Factors

Transfection of Hypoxia-Inducible Factor-1α mRNA Upregulates the Expression of Genes Encoding Angiogenic Growth Factors

Advances in the Preparation of Nanofiber Dressings by Electrospinning for Promoting Diabetic Wound Healing

Fabrication of an Adhesive Small Intestinal Submucosa Acellular Matrix Hydrogel for Accelerating Diabetic Wound Healing

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