Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Abstract

Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A165 on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A165 have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.

Complexation of CXCL12, FGF-2 and VEGF with Heparin Modulates the Protein Release from Alginate Microbeads

“…Cell adhesion is the predominant process during the first day of cell culture. In addition, FGF-2 has been observed to inhibit adhesion of ECs [ 65 ]. This effect may contribute to comparable cell indexes of the Hep-containing and pure Alg samples, although the Alg microbeads released a lower FGF-2 amount.…”

“…However, FGF-2 evinced an inhibitory effect on HUVEC adhesion but a strong enhancement of cell proliferation. In contrast, VEGF affected cell proliferation only moderately, with no negative effect on HUVEC adhesion [ 65 ]. These data correlate with our findings on proliferation in a nutrient-deprived culture medium, where FGF-2 in the eluates exhibited a stronger effect on HUVEC proliferation than VEGF, and the cells exposed to FGF-2 probably developed more cell–cell contacts that prevented the cell death.…”

Dual roles of oxostephanine as an Aurora kinase inhibitor and angiogenesis suppressor

“…Furthermore, the data indicated that oxostephanine decreased the production of VEGF-A, HGF and FGF-2, which functions in the proliferation, migration and tube formation processes (48)(49)(50)(51), by both hUVEcs and hFBs. Notably, in the present study, in hUVEcs, the mRNA expression of VEGF-A in cells treated with oxostephanine was not considerably altered; however, the expression of FGF-2 was significantly decreased compared to the control.…”

Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

“…No. 89050205) seeded in soft foams scaffolds, incubated in 24 or 96-well plates was measured with a resazurin assay according to Sedlář, et al [4]. ASCs were isolated from lipoaspirates and characterized according earlier study [5].…”