Growth, feed conversion and retention, and proximate of eel juvenile treated by immersion of recombinant giant grouper growth hormone

Abstract

<p>This study was aimed to determine the immersion dose of recombinant giant grouper growth hormone (rElGH) to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). Glass eel were fed on blood worm for the first month, and commercial diet for the second month of rearing. Fish rearing was performed in 60 L glass aquarium at density of 150 fish per aquarium. The results showed that higher in growth body weight was obtained in immersion dose of 12 mg/L, by increment of about 37.4% higher compared to that of control. The immersion dose of 12 mg/L also increased specific growth rate by 29.3% higher (p<0.05) compared to control. Furthermore, at that immersion treatment, feed conversion ratio of blood worm and commercial diet decreased by 33.7% and 25.6% compared to control, respectively. Protein (7.15±0.08%) and lipid (9.95±0.10%) retentions in 12 mg/L rElGH-treated fish were higher (p<0.05) than those of control (protein 6.17±0.07%; lipid 5.73±0.06%). Lower in protein content (12.73%), while higher content in lipid (8.35%) and crude carbohydrate (3.22%) were found in 12 mg/L rElGH-treated fish compared to those of control (13.24%, 5.90%, and 1.76%, respectively). Thus, in general, rElGH immersion dose of 12 mg/L could generate high performances of eel juvenile, and application of rElGH can be useful to increase aquaculture eel production.</p><p>Keywords: immersion dose, growth hormone, growth, glass eel</p>

Growth, biochemical composition, innate immunity and histological performance of the juvenile humpback grouper (Cromileptes altivelis) after treatment with recombinant fish growth hormone

“…Relative weight gain from the oral and injection doses above compared to CÀ were 40.25% and 38.77% respectively. Another study using rElGH was performed by Handoyo et al (2012) on Indonesian eel fingerlings by immersion, oral and a combination of immersion and oral administration; those treatments increased growth by 37.4%, 65.7% and 102.9% over control respectively. The same application methods were used by Subaidah (2013) for white shrimp, and the growth of the rElGH treatment groups were 109.9%, 17.7% and 40.1% higher than control respectively.…”

“…Studies investigating the use of rGH for promoting growth have been performed in various species, including salmonids (Sekine Mizukami, Nishi, Kuwana, Saito, Sato, Itoh & Kawauchi 1985), rainbow trout (Moriyama, Yamamoto, Sugimoto, Abe, Hirano & Kawauchi 1993), red seabream (Bin, Kang-sen, Ying-li, Hong-zhii, Zhen-hui, Yong, Shan, Rao & Pei-jun 2001), flounder (Liu, Zhang, Zang, Liu, Arunakumara, Xu & Zhang 2008), tilapia (Li, Bai, Jian, Ye, Lao, Li, Luo & Liang 2003;Acosta, Carpio, Besada, Morales, S anchez, Curbelo, Ayala & Estrada 2008), rabbitfish (Funkenstein, Dyman, Lapidot, de Jesus-Ayson, Gertler & Ayson 2005), goldfish (Promdonkoy, Warit & Panyim 2004), black seabream (Tsai, Hsih & Kuo 1997), Indonesian eel (Handoyo, Alimuddin & Utomo 2012), giant gourami (Irmawati, Alimuddin, Zairin, Suprayudi & Wahyudi 2012) and many other species. rGH is applied through intraperitoneal injection (Sekine et al 1985;Li et al 2003;Funkenstein et al 2005), oral delivery (Moriyama et al 1993;Tsai et al 1997) or immersion (Acosta et al 2008;Irmawati et al 2012).…”

STIMULASI PERTUMBUHAN JUVENIL ABALON, Haliotis squamata DENGAN PEMBERIAN HORMON REKOMBINAN IKAN rElGH

“…Berdasarkan hasil penelitian Irmawati et al (2012) diketahui bahwa tingkat produksi rGH ikan kerapu kertang (rElGH) pada bakteri Escherichia coli lebih tinggi dibandingkan dengan rGH ikan mas (rCcGH) dan ikan gurami (rOgGH), sehingga penggunaan rElGH lebih efisien daripada rCcGH dan rOgGH. Selain itu, bioaktivitas rElGH juga telah diuji pada ikan sidat dan secara signifikan menunjukkan peningkatan pertumbuhan sebesar 29,3% dibandingkan kontrol (Handoyo et al, 2012).…”

“…Hasil penelitian Handoyo et al (2012) menunjukkan bahwa ikan sidat yang diberi perlakuan perendaman menggunakan rElGH memiliki sintasan lebih tinggi dibandingkan kontrol. Efek peningkatan sintasan dilaporan pada ikan tilapia, Oreochromis hornorum (Acosta et al, 2009) dan pada ikan gurami (Alimuddin et al, 2011) yang menyatakan bahwa pemberian rGH pada larva dapat meningkatkan sintasan.…”

Respons Pertumbuhan Dan Keragaan Darah Dari Tiga Strain Ikan Nila Terhadap Pemberian Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang

“…Tingkat produksi dalam pembuatan rGH ikan kerapu kertang Epinephelus lanceolatus (rElGH) lebih tinggi dibandingkan rGH ikan mas Cyprinus carpio (rCcGH) dan rGH ikan gurami Osphronemus gouramy (rOgGH). Beberapa penelitian telah mampu membuktikan kemampuan rElGH dalam meningkatkan pertumbuhan ikan target, sebagaimana telah dilakukan oleh Handoyo et al (2012) pada ikan sidat, Hardiantho et al (2012) dan Muhammad (2014) pada ikan nila, Putra (2010), dan Safir (2012) pada ikan gurame.…”