Growth hormone-releasing hormone influences neuronal expression in the developing chick brain. I. Catecholaminergic neurons

Kentroti, Susan; Vernadakis, Antonia

doi:10.1016/0165-3806(89)90028-x

Cited by 36 publications

(9 citation statements)

References 33 publications

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“…We have previously found biochemically detectable levels of TH as early as 4 days of embryogenesis in the chick embryo (Kentroti and Vernadakis, 1989) and, in the present study, we report the presence of TH-immunoreactive neuroblasts in cultures derived from 3-day-old whole chick embryo, cultures which contain both neural crest and neural tube derivatives. Other investigators, as well, have demonstrated the presence of immunoreactive TH and its message very early in development (E11.5) in neural crest derivatives in the rat (Cochard et al, 1978;Jonakait et al, 1989).…”

Section: Discussionmentioning

confidence: 53%

Early neuroblasts are pluripotential: Colocalization of neurotransmitter and neuropeptides

Kentroti

Vernadakis

1995

J of Neuroscience Research

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This study was undertaken in order to establish the presence of pluripotential neuroblasts in the developing chick CNS. This has been suggested by our previous observations that expression of emerging neuronal phenotypes in the chick embryo CNS is affected by exposure to neurotrophic substances (i.e., GHRH, SRIF, NGF, EGF, muscle-derived factors) or neurotoxins such as ethanol. We have proposed that one mechanism whereby these substances elicit their effects is by shifting phenotypic expression in populations of pluripotential neuroblasts. In order to establish the presence of significant populations of pluripotential neuroblasts, cultures obtained from 3-day-old whole chick embryos (E3WE) were double-stained with antibodies to markers specific for four neuronal phenotypes in various permutations. Cultures at 6 DIV were tested for the presence of tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), gamma-aminobutyric acid (GABA), and somatostatin (SRIF) alone, and in various combinations. We observed a colocalization of all phenotypic markers within neuronal perikarya and processes in more than fifty percent of neuronal cells in these cultures. These data suggest that developing neuroblasts at this stage of embryogenesis possess the machinery necessary to adopt multiple neuronal phenotypes. The colocalization of neurotransmitter proteins in early neuroblasts (60 hr of embryogenesis) supports the recent concept that these substances themselves may influence phenotypic expression and also supports our idea that microenvironmental factors (i.e., ethanol, growth factors) provide signals which affect emerging phenotypes.

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Section: Discussionmentioning

confidence: 53%

Early neuroblasts are pluripotential: Colocalization of neurotransmitter and neuropeptides

Kentroti

Vernadakis

1995

J of Neuroscience Research

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“…The total number of BrdU+ cells counted represented that population of neuroblasts born during thefirst 3 days in culture (and surviving to 6 DIV) and made up an average of one fourth of the total neuergic (----, tyrosine hydroxylase activity) and GABAergic (-.-.-.-.-.-. , glutamate decarboxylase activity) phenotypes (composite taken from Kentroti and Vernadakis, 1989, 1990b roblasts . Within this population (neuroblasts born between 0-3 DZV) the neuronal markers were expressed in a differential fashion (Fig.…”

Section: Differential Development Of Neuronal Phenotypes In Culturementioning

confidence: 99%

Ethanol neurotoxicity in culture: Selective loss of cholinergic neurons

Kentroti

Vernadakis

1996

J. Neurosci. Res.

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“…The activity of TH was measured according to the method of Masserano et al [8], as modified by Kentroti and Vernadakis [9]. The procedure involves the recovery and assay of ,4 C0 2 after decarboxylation with partially purified hog aromatic Lamino acid decarboxylase of carboxy-labelled dihydroxyphenylalanine, formed from carboxy-labelled tyrosine.…”

Section: Acetyltransferase (Chat) Activitymentioning

confidence: 99%

Modification of the expression of adenosine 3′,5′-cyclic monophosphate-induced differentiated functions in neuroblastoma cells by beta-carotene and D-alpha-tocopheryl succinate.

Prasad

Kentroti

Edwards‐Prasad

et al. 1994

Journal of the American College of Nutrition

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Growth hormone-releasing hormone influences neuronal expression in the developing chick brain. I. Catecholaminergic neurons

Cited by 36 publications

References 33 publications

Early neuroblasts are pluripotential: Colocalization of neurotransmitter and neuropeptides

Early neuroblasts are pluripotential: Colocalization of neurotransmitter and neuropeptides

Ethanol neurotoxicity in culture: Selective loss of cholinergic neurons

Modification of the expression of adenosine 3′,5′-cyclic monophosphate-induced differentiated functions in neuroblastoma cells by beta-carotene and D-alpha-tocopheryl succinate.

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