Growth inhibition and coordinated physiological regulation of zebrafish (Danio rerio) embryos upon sublethal exposure to antidepressant amitriptyline

Yang, Ming; Qiu, Wenhui; Chen, Jingsi; Zhan, Jing; Pan, Chenyuan; Lei, Xiangjie; Wu, Minghong

doi:10.1016/j.aquatox.2013.12.029

Cited by 84 publications

(49 citation statements)

References 49 publications

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“…The decrease in GSH supports its utilization to neutralize ROS; however, not all exposures result in GSH decrease. For example, exposure to antidepressant amitriptyline resulted in a U-shape response, such that lower concentrations decreased GSH concentration, while higher concentrations increased GSH concentration (Yang et al, 2014). Exposures to bacterial lipopolysaccharides (Jaja-Chimedza et al, 2012) and total particulate matter from cigarette smoke (Massarsky et al, 2015) also appeared to elevate GSH, while exposures to β-naphthoflavone and α-naphthoflavone (Timme-Laragy et al, 2009) did not significantly affect GSH concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…GR activity has also been shown to decrease. For example, zebrafish exposed to bisphenol A and nonylphenol had significantly lower GR activity (Wu et al, 2011), whereas zebrafish exposed to amitriptyline showed decreased GR activity at lower concentrations and increased activity at the higher concentrations (Yang et al, 2014). …”

Section: Discussionmentioning

confidence: 99%

“…GSH and GSSG concentrations can also be evaluated using commercially available kits from Cayman Chemical Company (Ann Arbor, MI) as in Jaja-Chimedza et al (2012), Beyotime Institute of Biotechnology (Nantong, China) as in Yin et al (2014), as well as Nanjing Jiancheng Bioengineering Institute (Nanjing, China) as in Jin et al (2015a), Jin et al (2015b), Liu et al (2015), Si et al (2013), Wu et al (2011), and Yang et al (2014). Several kits are also available through Sigma-Aldrich (St. Louis, MO); however, to the best of our knowledge, these are yet to be used with zebrafish.…”

Section: Discussionmentioning

confidence: 99%

“…Activities of GPx and GR can also be evaluated using commercially available kits from Sigma-Alrich (St. Louis, MO) as in Jaja-Chimedza et al (2012), as well as Nanjing Jiancheng Bioengineering Institute (Nanjing, China) as in Wu et al (2011), Xu et al 2013, Yang et al 2014, Jin et al (2015a), and Jin et al (2015b). While commercially available kits are similarly easy to use and high-throughput, the methods presented herein are more cost-effective per sample, since most of the ingredients are routinely used in most labs.…”

Section: Discussionmentioning

confidence: 99%

“…While commercially available kits are similarly easy to use and high-throughput, the methods presented herein are more cost-effective per sample, since most of the ingredients are routinely used in most labs. Notably, gene expression related to antioxidant enzyme activity, measured by qRT-PCR analysis, is often presented in addition to activity results as in Yang et al (2014), Jin et al (2015a), and Jin et al (2015b). While gene expression may serve to validate activity results as well as provide additional mechanistic information, gene expression is a transitionary signature of the oxidative stress phenotype, and the results may be confounded by natural oscillations in gene expression patterns.…”

Section: Discussionmentioning

confidence: 99%

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Glutathione and zebrafish: Old assays to address a current issue

2017

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Several xenobiotic agents (e.g. metals, polycyclic aromatic hydrocarbons, nanoparticles, etc.) commonly involve the generation of reactive oxygen species (ROS) and oxidative stress as part of their toxic mode of action. Among piscine models, the zebrafish is a popular vertebrate model to study toxicity of various xenobiotic agents. Similarly to other vertebrates, zebrafish possess an extensive antioxidant system, including the reduced form of glutathione (GSH), which is an important antioxidant that acts alone or in conjunction with enzymes, such as glutathione peroxidase (GPx). Upon interaction with ROS, GSH is oxidized, resulting in the formation of glutathione disulfide (GSSG). GSSG is recycled by an auxiliary antioxidant enzyme glutathione reductase (GR). This article outlines detailed methods to measure the concentrations of GSH and GSSG, as well as the activities of GPx and GR in zebrafish larvae as robust and economical means to assess oxidative stress. The studies that have assessed these endpoints in zebrafish and alternative methods are also discussed. We conclude that the availability of these robust and economical methods support the use of zebrafish as a model organism in studies evaluating redox biology, as well as the induction of oxidative stress following exposure to toxic agents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Glutathione and zebrafish: Old assays to address a current issue

2017

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The effects of antidepressants appear to be rapid and at environmentally relevant concentrations

Ford

Fong

2015

Enviro Toxic and Chemistry

105

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The effects of antidepressants on wildlife are currently raising some concern because of an increased number of publications indicating biological effects at environmentally relevant concentrations (<100 ng/L). These results have been met with some scepticism because of the higher concentrations required to detect effects in some species and the perceived slowness to therapeutic effects recorded in humans and other vertebrates. Because their mode of action is thought to be by modulation of the neurotransmitters serotonin, dopamine, and norepinephrine, aquatic invertebrates that possess transporters and receptors sensitive to activation by these pharmaceuticals are potentially affected by them. The authors highlight studies on the effects of antidepressants, particularly on crustacean and molluskan groups, showing that they are susceptible to a wide variety of neuroendocrine disruptions at environmentally relevant concentrations. Interestingly, some effects observed in these species can be observed within minutes to hours of exposure. For example, exposure of amphipod crustaceans to several selective serotonin reuptake inhibitors can invoke changes in swimming behavior within hours. In mollusks, exposure to selective serotonin reuptake inhibitors can induce spawning in male and female mussels and foot detachment in snails within minutes of exposure. In the light of new studies indicating effects on the human brain from selective serotonin reuptake inhibitors using magnetic resonance imaging scans, the authors discuss possible reasons for the discrepancy in former results in relation to the read-across hypothesis, variation in biomarkers used, modes of uptake, phylogenetic distance, and the affinity to different targets and differential sensitivity to receptors. Environ Toxicol Chem 2016;35:794-798. #

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Amitriptyline at an Environmentally Relevant Concentration Alters the Profile of Metabolites Beyond Monoamines in Gilt‐Head Bream

Ziarrusta

Ribbenstedt

Mijangos

et al. 2019

Enviro Toxic and Chemistry

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The antidepressant amitriptyline is a widely used selective serotonin reuptake inhibitor that is found in the aquatic environment. The present study investigates alterations in the brain and the liver metabolome of gilt‐head bream (Sparus aurata) after exposure at an environmentally relevant concentration (0.2 µg/L) of amitriptyline for 7 d. Analysis of variance–simultaneous component analysis is used to identify metabolites that distinguish exposed from control animals. Overall, alterations in lipid metabolism suggest the occurrence of oxidative stress in both the brain and the liver—a common adverse effect of xenobiotics. However, alterations in the amino acid arginine are also observed. These are likely related to the nitric oxide system that is known to be associated with the mechanism of action of antidepressants. In addition, changes in asparagine and methionine levels in the brain and pantothenate, uric acid, and formylisoglutamine/N‐formimino‐L‐glutamate levels in the liver could indicate variation of amino acid metabolism in both tissues; and the perturbation of glutamate in the liver implies that the energy metabolism is also affected. These results reveal that environmentally relevant concentrations of amitriptyline perturb a fraction of the metabolome that is not typically associated with antidepressant exposure in fish. Environ Toxicol Chem 2019;00:1–13. © 2019 SETAC

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Growth inhibition and coordinated physiological regulation of zebrafish (Danio rerio) embryos upon sublethal exposure to antidepressant amitriptyline

Cited by 84 publications

References 49 publications

Glutathione and zebrafish: Old assays to address a current issue

Glutathione and zebrafish: Old assays to address a current issue

The effects of antidepressants appear to be rapid and at environmentally relevant concentrations

Amitriptyline at an Environmentally Relevant Concentration Alters the Profile of Metabolites Beyond Monoamines in Gilt‐Head Bream

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