Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit

Leelasuphakul, Wichitra; Hemmanee, Punpen; Chuenchitt, S.

doi:10.1016/j.postharvbio.2007.09.024

Cited by 209 publications

(135 citation statements)

References 39 publications

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“…These strains were shown to be Fengycin A and/or Bacillomycin D producers (Table 1). Microorganisms acting through antibiosis, generally have a wide action spectrum, and thus pathogen inhibition by producing toxic substances is more effective than any other mechanism of action [38]. These cyclic lipopeptide antibiotics have been reported to inhibit various phytopathogenic fungi including S. sclerotiorum [39,40].…”

Section: Discussionmentioning

confidence: 99%

Bio-suppression of Sclerotinia Stem Rot of Tomato and Biostimulation of Plant Growth Using Tomato-associated Rhizobacteria

Abdeljalil¹,

Vallance²

2016

J Plant Pathol Microbiol

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Section: Discussionmentioning

confidence: 99%

Bio-suppression of Sclerotinia Stem Rot of Tomato and Biostimulation of Plant Growth Using Tomato-associated Rhizobacteria

Abdeljalil¹,

Vallance²

2016

J Plant Pathol Microbiol

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“…To reach the best separation effect, the developing solvent of chloroform, methyl alcohol and water in 65: 25: 4 ratio was used for extracting the lipopeptides from thin layer chromatography on a silica gel was performed, and the Rf value of a protein band was obtained according to the surfactin standard (Leelasuphakul et al, 2008). This further confirmed that the antibacterial substance contained micromocular lipopeptides, proving that CMN1308 strain could produce surfactin preliminarily.…”

Section: Purification Of Antibacterial Substances Of Strain Cmn1308mentioning

confidence: 70%

Extraction, Isolation and Identification of Antimicrobial Substances from Bacillus amyloliquefaciens CMN1308

Fang

Zheng

et al. 2017

Not Bot Horti Agrobo

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Four separation methods of antimicrobial substances produced by CMN1308 (Bacillus amyloliquefaciens) were evaluated and selected according to number of antimicrobial substances and its activity in vitro. The results showed that extraction by acid precipitation of the fermentation supernatant of CMN1308 was the best with a diameter of inhibition zone of pathogen fungi P. expansum of 12.3 mm in a laboratory bioassay. Applying a silica thin layer chromatography (TLC), SDS-PAGE and other separation technologies we isolate antimicrobial substances, and the separated band were cut off for mass spectrometry analysis. The TLC of crude extract of CMN1308 show a topical band corresponding with the surfactin standard (Rf value =0.75), proved that the strain CMN1308 can produce this surface active compound. The mycoprotein extracted from CMN1308 was separated by Tricine-SDS-PAGE modified with the addition of urea in the separation gel. After mass spectrometric analysis and protein characterization, the isolated mycoprotein showed a maximum ion peak at M/Z of 2679 and molecular weight of 29.5 kDa, matching with protein flagellin. The extracellular antimicrobial protein of strain CMN1308 display four bands after urea-Tricine-SDS-PAGE, but after mass spectrometry analysis only two bands were identified. Band "A" with a maximum ion peak at M/Z of 1926 and molecular weight of 49.8 kDa, aligned with NCBI database, matching with DLDH (dihydrolipoamide dehydrogenase enzyme). Band "D" show the maximum ion peak at M/Z of 2936 and molecular weight of 22.4 kD, matching with a chitin binding protein. Thus, the strain CMN1308 has the potential to be developed as a commercial biological control agent for chestnut common pathogenic fungi.

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“…Em pós-colheita, Hao et al (2011) verificaram redução na incidência das doenças causadas por P. digitatum e P. italicum, CONTROLE EM PÓS-COLHEITA DE Penicillium digitatum EM LARANJA-PERA.. quando os frutos de tangerina foram tratados com Bacillus amyloliquefaciens (HF-01), antes da inoculação dos patógenos. Leelasuphakul et al (2008) verificaram que endósporos de B. subtilis (155) ou seus metabólitos reduziram a incidência de severidade e retardaram o início do aparecimento dos sintomas do bolor verde em frutos cítricos. O controle só ocorreu quando os frutos foram tratados antes (melhor ação de endósporos) ou junto (melhor ação dos metabólitos secundários) da inoculação com patógeno.…”

Section: Resultsunclassified

“…(P39, P40 e P41) (ZAMANI et al, 2008) e Bacillus spp. (LEELASUPHAKUL et al, 2008) são exemplos de antagonistas eficientes em pós-colheita contra diversos fitopatógenos.…”

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Controle em pós-colheita de Penicillium digitatum EM laranja-pera com microrganismos e tratamento térmico

Forner¹,

Bettiol

Nascimento³

et al. 2013

Rev. Bras. Frutic.

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INTRODUÇÃODoenças em pós-colheita reduzem a quantidade e a qualidade de frutos comercializáveis e, em citros, podem provocar grandes perdas. No Brasil, dentre as doenças em pós-colheita dos citros, o bolor verde (Penicillium digitatum) é a principal. Seu controle é realizado com fungicidas, e isolados resistentes do patógeno são relatados (TEIXIDÓ et al., 2001), algumas vezes simultânea a mais de um fungicida (KINAY et al., 2007 RESUMO-O mercado consumidor está exigindo alimentos sem a presença de resíduos de agrotóxicos. Assim, o trabalho teve como objetivo avaliar o controle do bolor verde, em laranjas-Pera, com agentes de biocontrole (Bacillus subtilis e Bacillus licheniformis, Bacillus subtilis (QST 713)), associados ou não ao tratamento térmico. Para tanto, os frutos foram adquiridos em "packinghouse" antes do processamento, sendo lavados e desinfestados com hipoclorito de sódio. Os frutos submetidos a esses tratamentos foram armazenados, por 11 a 28 dias, em temperatura de 10 ºC e UR 90%±5 ou por oito dias a 20 ºC e UR 90%±5. De modo geral, o tratamento térmico reduziu a severidade da doença determinada pela área abaixo da curva do progresso da doença nos frutos e a incidência natural de doenças em pós-colheita de laranja-Pera. Por outro lado, os agentes de biocontrole não controlaram a doença, mostrando que os organismos testados não apresentaram atividade curativa contra o bolor verde. Termos de indexação: Bolor verde, controle biológico, controle físico, citros. POSTHARVEST CONTROL OF Penicillium digitatum IN PERA ORANGE TREES WITH MICROORGANISMS AND HEAT TREATMENTABSTRACT-The consumer market demands food without pesticide residues. Therefore, this study focused on evaluating the control of green mold in Pera orange trees with biocontrol agents (Bacillus subtilis, Bacillus licheniformis and Bacillus subtilis (QST 713)), associated or not with heat treatment. The fruit was obtained in packinghouse before processing, being washed and disinfected with the use of Sodium Hypochlorite. Fruits submitted to these treatments were stored from 11 to 28 days at temperature of 10 ºC and RH 90%±5 or for eight days at 20 ºC and 90%±5. In general, the heat treatment reduced the disease severity determine by the area under the disease progress curve in the fruit and the incidence of natural postharvest disease in Pera oranges. On the other hand, biocontrol agents did not control the disease, showing that the organisms tested did not present curative activity against the green mold.

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Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit

Cited by 209 publications

References 39 publications

Bio-suppression of Sclerotinia Stem Rot of Tomato and Biostimulation of Plant Growth Using Tomato-associated Rhizobacteria

Bio-suppression of Sclerotinia Stem Rot of Tomato and Biostimulation of Plant Growth Using Tomato-associated Rhizobacteria

Extraction, Isolation and Identification of Antimicrobial Substances from Bacillus amyloliquefaciens CMN1308

Controle em pós-colheita de Penicillium digitatum EM laranja-pera com microrganismos e tratamento térmico

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