1977
DOI: 10.1016/s0015-3796(17)30311-6
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Growth, Phosphatase and Ribonuclease Activity in Phosphate-deficient Spirodela oligorrhiza Cultures

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Cited by 18 publications
(8 citation statements)
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“…An index of the chlorophyll content in leaves was estimated using a SPAD-502 chlorophyll meter (Konica Minolta, Osaka, Japan) [ 17 ]. The activities of acid (pH 6) (EC 3.1.3.2) and alkaline (pH 7.5) (EC 3.1.3.1) phosphorylases (U g −1 (FM) min −1 ) in leaves and RNase (EC 3.1.27.5) (U g −1 (FM) min −1 ) were studied using the methods demonstrated by Knypl and Kabzinska [ 50 ]. The activity of total dehydrogenases (EC 1.1.1.-) was measured using the procedure presented by Górnik and Grzesik [ 20 , 51 ] with a spectrophotometer (UVmini-1240, Shimadzu, Japan) for formazan determination at a wavelength of 480 nm.…”
Section: Methodsmentioning
confidence: 99%
“…An index of the chlorophyll content in leaves was estimated using a SPAD-502 chlorophyll meter (Konica Minolta, Osaka, Japan) [ 17 ]. The activities of acid (pH 6) (EC 3.1.3.2) and alkaline (pH 7.5) (EC 3.1.3.1) phosphorylases (U g −1 (FM) min −1 ) in leaves and RNase (EC 3.1.27.5) (U g −1 (FM) min −1 ) were studied using the methods demonstrated by Knypl and Kabzinska [ 50 ]. The activity of total dehydrogenases (EC 1.1.1.-) was measured using the procedure presented by Górnik and Grzesik [ 20 , 51 ] with a spectrophotometer (UVmini-1240, Shimadzu, Japan) for formazan determination at a wavelength of 480 nm.…”
Section: Methodsmentioning
confidence: 99%
“…The activity of net photosynthesis, stomatal conductance, intercellular CO 2 concentration, and transpiration were measured in the infrared light using a TPS-2 gas analyzer apparatus, PP Systems (USA) [20,21]. Activities of acid (pH=6) and alkaline (pH=7.5) phosphatase and RNase in the leaves were examined according to the methods described by Knypl and Kabzińska [22]. Total dehydrogenase activities were evaluated by the procedure described by Górnik and Grzesik [23] using SHIMADZU UVmini-1240 spectrophotometer for formazan determination at a wavelength of 480 nm.…”
mentioning
confidence: 99%
“…The electrolyte leakage was measured after 2 and 4 h, using microcomputer conductivity meter CC-551 Elmetron (Gornik and Grzesik, 2002;Szwonek, 2009). The activities of acid and alkaline phosphatases and RNase in the leaves were examined according to the methods described by Knypl and Kabzinska (1977) (Smolen, 2009). The index of chlorophyll content in the leaves was evaluated using Minolta SPAD-502, Japan.…”
Section: Methodsmentioning
confidence: 99%