Growth, Water Content, and Proline Accumulation in Drought-Stressed Callus of Date Palm

Al-Khayri, Jameel M.; Al-Bahrany, Abdulaziz M.

doi:10.1023/b:biop.0000024283.74919.4c

Cited by 59 publications

(25 citation statements)

References 21 publications

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“…Proline concentration in date palm increased significantly under salinity [30] and drought stress [31], so that exposure to low intensity MF may be a convenient method to select for tolerance to these abiotic stresses. At high intensity of SMF or AMF, the proline mechanism appears to fail, yet as exposed seedlings develop normally, this suggests that other protective mechanisms may be involved, perhaps involving oxidizing enzymes [3,10] or another cellular solute such as alanine [1].…”

Section: Resultsmentioning

confidence: 99%

“…In studies on date palm (Phoenix dactylifera L.), an economically important species adapted to environmental stress factors associated with the hot arid regions where it is predominately grown, proline accumulation was observed in callus cultures subjected to salinity [30] and drought conditions [31]. However, we have not encountered any literature relating to the effect of magnetic field on proline accumulation in plant systems.…”

Section: Introductionmentioning

confidence: 97%

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Proline Accumulation in Response to Magnetic Fields in Date Palm (Phoenix dactylifera L.)

Dhawi¹,

Al-Khayri²

2008

TOASJ

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Proline accumulation is a common biochemical indicator for assessing environmental stress in plants. The objective of this study was to determine the effect of various doses of two types of magnetic fields on date palm (Phoenix dactylifera L. (cv. Khalas) seedlings based on proline accumulation. The first type involved static magnetic field (SMF) generated by an electromagnetic circuit set at 10, 50, and 100 mT for 30, 60, 120, 180, 240 and 360 min. The second type involved alternating magnetic field (AMF) generated by a magnetic resonance imaging (MRI) device set at 1.5 T for 1, 5, 10, and 15 min. Following exposure to radiation, the seedlings were grown in potting soil for 4 weeks after which proline analysis was conducted. The results showed a significant two-way interaction between SMF intensity and exposure duration. At the lowest intensity, 10 mT, proline concentration increased in response to longer exposure durations reaching a maximum at 240 min. Beyond this duration, reduction in proline concentration occurred. In contrast, at 50 and 100 mT, proline concentration decreased as the exposure duration was increased. AMF significantly reduced proline concentration after as short as a 1-min exposure. This study would facilitate investigations related to stress physiology and in vitro selection of date palm tissue culture.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Proline Accumulation in Response to Magnetic Fields in Date Palm (Phoenix dactylifera L.)

Dhawi¹,

Al-Khayri²

2008

TOASJ

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“…The relative water content (RWC) of callus was calculated using the formula (FW -DW) / DW; where FW D fresh weight, DW D dry weight and percentage value was determined following the method of Al-Khayri and Al-Bahrany. 69 Data recording and statistical analysis The average or mean values were computed from 3 replications with standard error (SE). The experiments were laid out as completely randomized design (CRD).…”

Section: Sterilization Of Seeds and Inoculationmentioning

confidence: 99%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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D number of viable callus; ICn D number of inoculated callus.Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL ¡1) and Kin (1.0 mgL ¡1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL ¡1 ) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL ¡1 NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL ¡1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

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“…3,4,5 Al-Khayri and A-Bahrany. 6 stated that water availability is one of the principal limitations of crop production, particularly in the arid and semiarid regions where date palm is predominantly grown. Due to its huge size, most physiological studies on date palm trees are achieved on seedling which originated from seed or tissue culture.…”

Section: Phoenix Dactylifera L (Date Palm) Belongs To the Genus Phoementioning

confidence: 99%

The Antioxidant Enzymatic Activity of Date Palm Seedlings Under Abiotic Drought Stress

Sakran¹,

Rabey²,

Almulaiky³

et al. 2018

IJPER

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Objective: the present research was focused to evaluate the date palm resistance to osmotic stress exerted by three concentrations of poly ethylene glycol 6000 in three months seedlings as reflected by the change in phenolic and flavonoid content and the activity of catalase, peroxidase and polyphenol oxidase. Materials and Methods: The phenolic and flavonoid content and the target enzyme's activity was estimated at four time points; 0,1,3,7 days. Results and Discussion: The phenolic and flavonoid content as well as the activity of catalase, peroxidase and polyphenol oxidase were increased by increasing the poly ethylene glycol and the treatment period. Conclusion: Phenolic and flavonoids are non-enzymatic antioxidants with high scavenging potential to the free radical produced from the oxidative stress resulted from the drought tolerance in date palm seedlings. In addition, the increase in the enzymatic activity of catalase, peroxidase and polyphenol oxidase under drought stress is very important to get rid of the free radicals and the reactive oxygen species.

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Growth, Water Content, and Proline Accumulation in Drought-Stressed Callus of Date Palm

Cited by 59 publications

References 21 publications

Proline Accumulation in Response to Magnetic Fields in Date Palm (Phoenix dactylifera L.)

Proline Accumulation in Response to Magnetic Fields in Date Palm (Phoenix dactylifera L.)

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

The Antioxidant Enzymatic Activity of Date Palm Seedlings Under Abiotic Drought Stress

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