Guidance of neurite outgrowth by pathways of substratum‐adsorbed laminin

Hammarback, James A.; Palm, Sally L.; Furcht, Leo T.; Letourneau, Paul C.

doi:10.1002/jnr.490130115

Cited by 246 publications

(121 citation statements)

References 17 publications

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“…Cooper et al 107 vacuum deposited thin films of silicon monoxide through masks and found that mouse neuroblastoma cells NB41A adhered and neurites aligned on the silicon monoxide lines. Hammarback et al 108 created micropatterns of "bioactive" and inactive laminin by ultraviolet light inactivation of laminin-coated coverslips masked with EM grids; they found that axon growth cone guidance of chicken embryonic DRG neurons by substrate-bound laminin pathways was correlated with neuron-to-pathway adhesivity 109 By UV irradiation of agarose/albumin surface through EM grids followed with laminin absorption on the irradiated region, they also created patterns of laminin-coated (high adhesivity) surfaces interspersed with agarose/albumin (low adhesivity)-coated regions. 110 Neurons only adhered to the laminin-coated regions, but some neurites extended across intervening agarose/ albumin regions, supporting the guidepost hypothesis for pioneer axon pathfinding.…”

Section: Vb Neuronal Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Vb Neuronal Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…Several methods have been developed for patterning surfaces to control neuronal attachment and growth (Hammarback et al, 1985;Kleinfeld et al, 1988;Corey et al, 1991;Stenger et al, 1992;Matsuda et al, 1992;Lom et al, 1993;Hickman et al, 1994;Corey et al, 1997;Branch et al, 1998;Ravenscroft et al, 1998;Branch et al, 2000;James et al, 2000;Lauer et al, 2001). Using these techniques it is possible to direct neuron cell bodies to electrodes and thus provide the ability to study individual neurons within a neural network.…”

Section: Introductionmentioning

confidence: 99%

Low-density neuronal networks cultured using patterned poly-l-lysine on microelectrode arrays

Jun

Hynd

Dowell-Mesfin

et al. 2007

Journal of Neuroscience Methods

101

100

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Synaptic activity recorded from low-density networks of cultured rat hippocampal neurons was monitored using microelectrode arrays (MEAs). Neuronal networks were patterned with poly-Llysine (PLL) using microcontact printing (µCP). Polydimethysiloxane (PDMS) stamps were fabricated with relief structures resulting in patterns of 2 µm-wide lines for directing process growth and 20 µm-diameter circles for cell soma attachment. These circles were aligned to electrode sites. Different densities of neurons were plated in order to assess the minimal neuron density required for development of an active network. Spontaneous activity was observed at 10-14 days in networks using neuron densities as low as 200 cells/mm 2 . Immunocytochemistry demonstrated the distribution of dendrites along the lines and the location of foci of the presynaptic protein, synaptophysin, on neuron somas and dendrites. Scanning electron microscopy demonstrated that single fluorescent tracks contained multiple processes. Evoked responses of selected portions of the networks were produced by stimulation of specific electrode sites. In addition, the neuronal excitability of the network was increased by the bath application of high K + (10-12 mM). Application of DNQX, an AMPA antagonist, blocked all spontaneous activity, suggesting that the activity is excitatory and mediated through glutamate receptors.

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“…Zn vivo the ECM is involved in the migration of neural crest cells during development (Boucaut et al, 1984;Bronner-Fraser, 1986), as well as in the regeneration of peripheral nerves (Sandrock and Matthew, 1987) and myoneural synapses (Nitkin et al, 1983). In cell culture, the adhesive interactions of neurons with purified ECM molecules such as collagen, fibronectin, and laminin strongly influence the extent (Akers et al, 198 1;Carbonetto et al, 1982Carbonetto et al, , 1983Man-thorpe et al, 1983) and direction (Turner and Carbonetto, 1984;Hammarback et al, 1985) of neurite growth. Laminin, in particular, stimulates neurite outgrowth from dorsal root or sympathetic ganglia, which otherwise require exogenous nerve growth factor (NGF) (Edgar et al, 1984;Lander et al, 1985).…”

mentioning

confidence: 99%

Identification of a cell-surface protein involved in PC12 cell- substratum adhesion and neurite outgrowth on laminin and collagen

1989

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On substrata coated with laminin or native collagen (Types I/III), PC12 cells employ an active adhesion mechanism (i.e., one inhibited at low temperature, by azide or in the absence of divalent cations) to attach and extend neurites; on substrata coated with wheat germ agglutinin (WGA) or polylysine, by contrast, PC12 cells attach via a passive mechanism and fail to extend neurites (Turner et al., 1987). This paper reports the isolation of 2 monoclonal antibodies (3A3 and 1B1) that promote retraction of neurites extended on laminin and collagen. In studies of initial cell attachment, 3A3 inhibited active attachment to laminin or collagen but not passive attachment to WGA or polylysine, whereas 1B1 inhibited both active and passive attachment. The more potent of the antibodies, 3A3, precipitates 2 radioactive protein bands (of approximately 185 and 125 kDa) from 1% Nonidet P-40 extracts of metabolically labeled PC12 cells. The properties of these proteins suggest that the antigen recognized by 3A3 is a member of the integrin family of matrix receptors. The other monoclonal antibody, 1B1, reacts with many PC12 proteins, including both bands precipitated by 3A3. The available data strongly suggest that an integrin with specificity for both laminin and collagen mediates PC12 adhesion to the substratum at both the cell body and the neurite growth cone.

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Guidance of neurite outgrowth by pathways of substratum‐adsorbed laminin

Cited by 246 publications

References 17 publications

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Low-density neuronal networks cultured using patterned poly-l-lysine on microelectrode arrays

Identification of a cell-surface protein involved in PC12 cell- substratum adhesion and neurite outgrowth on laminin and collagen

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