Gynogenetic diploid production in the red sea bream using UV-irradiated sperm of black sea bream and heat shock.

Sugama, Ketut; Taniguchi, Nobuyuki; Seki, Singo; Nabeshima, Hiroshi; Hasegawa, Yoshio

doi:10.2331/suisan.56.1427

Cited by 16 publications

(9 citation statements)

References 7 publications

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“…This finding is in agreement with similar experiments of chromosome manipulation in the red sea bream (SUGAMA et al 1990a;KITAMURA et al 1991;SUGAMA et al 1992). Thus, cold shocking is a valid alternative to heat shocking as tested in both sea bass (CARRILLO et al 1993) and other marine teleosts (SUGAMA et al 1990b). The low percentage of triploidy in our large-scale trial was probably caused by some technical bias in the handling of a greater egg mass.…”

supporting

confidence: 92%

Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass, Dicentrarchus labrax L.

Colombo

Barbaro²,

Libertini³

et al. 1995

J Appl Ichthyol

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The European sea bass, Dicentrarchus labrax L., was successfully subjected to chromosome manipulation. Triploidy was induced by cold-shocking e gs at 0-2°C for 20 min starting 5 min after fertilization in order to prevent the extrusion of the secon%polar body. Meiogynogenesis was also obtained by fertilizing eggs with W-irradiated sperm (3300 and 6600 erg m-) and subsequently doublin the chromosome set by meiotic block as above. The commercial advantages of culturing triploicf and gynogenetic sea bass are discussed.

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supporting

confidence: 92%

Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass, Dicentrarchus labrax L.

Colombo

Barbaro²,

Libertini³

et al. 1995

J Appl Ichthyol

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“…Lower growth performance of gynogenetic diploids in comparison with diploid controls has been reported in Misgurnus anguillicaudatus (Cantor) (Suzuki et al, 1985) and Salmo salar L. (Johnstone & Stet, 1995). In Pagrus major Temminck & Schlegel (Sugama et al, 1990), Clarias macrocephalus Gü nther (Na-Nakorn, 1995) and Gnathopogon caerulescens Sauvage (Fujioka, 1998) similar growth was obtained between gynogenetics and controls. In this study, there were no significant differences between the diploid control and the meiogynogenetic sea bass originating from the same breeders, but there were significant differences between the performance of progenies originating from different breeders.…”

Section: Discussionsupporting

confidence: 69%

“…In this study, there were no significant differences between the diploid control and the meiogynogenetic sea bass originating from the same breeders, but there were significant differences between the performance of progenies originating from different breeders. This suggests that observed differences in growth between the two progenies may be due to genetic factors from individual females (Sugama et al, 1990;Fujioka, 1998), indicating that phenotypic growth is more dependent on maternal effects than on effects of induction in the gynogenetics. On the other hand, despite the fact that fish were produced under optimal treatment conditions and reared under standard procedures, the number of viable fish was low.…”

Section: Discussionmentioning

confidence: 99%

Growth, gonadal development and sex ratios of meiogynogenetic diploid sea bass

Felip

Piferrer

Carrillo

et al. 2002

Journal of Fish Biology

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Gynogenesis showed little effect on general physiology and gonadal development in sea bass Dicentrarchus labrax. Meiogynogenetic fish showed well-developed gonads indicating low occurrence of developmental imbalances even after gynogenesis induction in this species. In addition, the proportion of sexes of meiogynogenetic sea bass was similar to the diploid controls in two independent trials, which did not deviate significantly from a 1 : 1 male : female sex ratio. Even considering some environmental influence on sex differentiation, as has been previously demonstrated, the fact that the proportion of sexes was similar between gynogenetic and control diploids essentially eliminates the possibility that in the sea bass the females are the homogametic sex. Although the mechanism of sex determination of this species still remains unknown, even after gynogenesis induction, the genetic mechanism of the ZW/ZZ type could probably operate in the sea bass.

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“…In red sea bream Pagrus major Temminck and Schlegel, no differences in growth rates were found between control and gynogenetic offspring up to the age of 6 months (Sugama et al, 1990). In contrast, in loach Misgurnus anguillicaudatus (Cantaor), Suzuki et al (1985) reported that the different growth rates between control and gynogen fish observed after 12 weeks were caused by a high level of abnormal fish among the gynogens.…”

Section: Discussionmentioning

confidence: 94%

“…Therefore, to ensure that paternal chromosomes are not transmitted to the next generation, sperm from a different fish species, given that no viable hybrids are produced, was used (Chourrout, 1986;Varadaraj, 1990;Sugama et al, 1990;Kavumpurath & Pandian, 1994;Smoker et al, 1995;Linhart et al, 1995;Mims et al, 1997;Fujioka, 1998;Gomelsky et al, 1998;Kawamura, 1998). Thus, individual embryos arising accidentally from intact sperm will be eliminated by lethal hybridization (Chourrout, 1986).…”

Section: Introductionmentioning

confidence: 99%

Induction of gynogenesis and gonad development in the muskellunge

Rlnchard

Garcia-Abiado

Dąbrowski

et al. 2002

Journal of Fish Biology

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Muskellunge Esox masquinongy eggs activated with UV-irradiated yellow perch Perca flavescens sperm were subjected to heat shock at 31 1 C for 6 min, 20 min after fertilization in three experiments. Survival at eyed stage was 1·7 1·6, 6·8 4·8 and 2·3 0·5% in experiments I, II and III, respectively. After rearing the gynogenetic muskellunge in troughs and then in ponds, the sex ratio of gynogens in experiment I did not significantly (P>0·05) differ from the expected ratio 0 : 1 (male : female), however, one male and one intersex were observed. In experiments II and III, the sex ratio of gynogens differed significantly from expected (0 : 1). Three months after hatching, the growth of females did not significantly (P>0·05) differ among control and gynogenetic groups, whereas male growth was significantly (P<0·01) higher in the control v. gynogenetic group. The histological structures of the gynogenetic fish gonads in both sexes were similar to those described in the gonads of control muskellunge. After the overwintering period, signs of active spermatogenesis were observed in the testis of 1+ year old gynogenetic fish, whereas ovaries contained only oocytes at the perinucleolar stage. At this stage, plasma sex steroids testosterone (T) and oestradiol-17 (E2) cannot be used to discriminate the sex of gynogenetic muskellunge, as intersex, male and female fish had similar levels of T and E2.

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Gynogenetic diploid production in the red sea bream using UV-irradiated sperm of black sea bream and heat shock.

Cited by 16 publications

References 7 publications

Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass, Dicentrarchus labrax L.

Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass, Dicentrarchus labrax L.

Growth, gonadal development and sex ratios of meiogynogenetic diploid sea bass

Induction of gynogenesis and gonad development in the muskellunge

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