2006
DOI: 10.1021/bm060718s
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H NMR Investigation of Thermally Triggered Insulin Release from Poly(N-isopropylacrylamide) Microgels

Abstract: We describe investigations of insulin release from thermoresponsive microgels using variable temperature (1)H NMR. Microgel particles composed of poly(N-isopropylacrylamide) were loaded with the peptide via a swelling technique, and this method was compared to simple equilibrium partitioning. Variable temperature (1)H NMR studies suggest that the swelling loading method results in enhanced entrapment of the peptide versus equilibrium partitioning. A centrifugation-loading assay supports this finding. Pseudo-te… Show more

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Cited by 96 publications
(67 citation statements)
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“…Previously, it was observed that when swollen particles are subjected to a temperature above LCST of PNIPAM, the particles shrink. The phenomenon happens as a result of disruption of hydrogen bonding with water molecules and the formation of hydrophobic associations among isopropyl moieties of PNIPAM [45,46]. Consequently, a resistance to flow and hence increase in viscosity at low shear rates can be observed due to formation of compact particles [47].…”
Section: Effect Of Temperature and Chemical Composition On The Rheolomentioning
confidence: 99%
“…Previously, it was observed that when swollen particles are subjected to a temperature above LCST of PNIPAM, the particles shrink. The phenomenon happens as a result of disruption of hydrogen bonding with water molecules and the formation of hydrophobic associations among isopropyl moieties of PNIPAM [45,46]. Consequently, a resistance to flow and hence increase in viscosity at low shear rates can be observed due to formation of compact particles [47].…”
Section: Effect Of Temperature and Chemical Composition On The Rheolomentioning
confidence: 99%
“…In aqueous solutions, a PNIPAM chain undergoes a well-characterized and reversible coilto-globule transition at its lower critical solution temperature (LCST), at which the 3D crosslinked PNIPAM networks exhibit a volume phase transition between swollen and collapsed phases [10]. Most of thermoresponsive microgels have been fabricated based on PNIPAM, its derivatives, or their hybrids [11][12][13][14][15][16]. Biodegradability is one of the most important considerations of scaffolds for tissue engineering.…”
Section: Introductionmentioning
confidence: 99%
“…9,10 In addition, their ability to bind and store substances and release them upon stimuli makes such systems potential candidates for drug delivery systems, not the least for protein and peptide drugs. 4,[11][12][13][14][15][16][17][18][19] When charged peptides interact with oppositely charged microgels, osmotic deswelling of the gel network takes place. However, due to the interplay between gel deswelling and peptide transport through the network, this is a complex process where a number of parameters effect the outcome in terms of functional properties such as the amount peptide incorporated, peptide distribution, and release rate.…”
Section: Introductionmentioning
confidence: 99%