H2O2 Signaling‐Triggered PI3K Mediates Mitochondrial Protection to Participate in Early Cardioprotection by Exercise Preconditioning

Yang, Yuan; S, Pan; Wan, Dong-Feng; Lu, Jiao; Huang, Yue

doi:10.1155/2018/1916841

Cited by 22 publications

(24 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein levels of Beclin 1 were downregulated for the ET group at 0 h but without significant alteration at 6 h. Furthermore, the ET group showed lower values of ATG5 at 6 h. Yuan et al [ 54 ] did not observe modifications in Beclin 1 in the heart tissue of rats after acute exhaustive treadmill running. Campos et al [ 55 ] blocked the autophagic flux with chloroquine and visualized an increase in LC3II, Sqstm1/p62, and Beclin 1 in control animals, but not in heart failure animals.…”

Section: Discussionmentioning

confidence: 97%

Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice

Pinto

Rocha

Marafon

et al. 2021

IJMS

View full text Add to dashboard Cite

Although physical exercise-induced autophagy activation has been considered a therapeutic target to enhance tissue health and extend lifespan, the effects of different exercise models on autophagy in specific metabolic tissues are not completely understood. This descriptive investigation compared the acute effects of endurance (END), exhaustive (ET), strength (ST), and concurrent (CC) physical exercise protocols on markers of autophagy, genes, and proteins in the gastrocnemius muscle, heart, and liver of mice. The animals were euthanized immediately (0 h) and six hours (6 h) after the acute exercise for the measurement of glycogen levels, mRNA expression of Prkaa1, Ppargc1a, Mtor, Ulk1, Becn1, Atg5, Map1lc3b, Sqstm1, and protein levels of Beclin 1 and ATG5. The markers of autophagy were measured by quantifying the protein levels of LC3II and Sqstm1/p62 in response to three consecutive days of intraperitoneal injections of colchicine. In summary, for gastrocnemius muscle samples, the main alterations in mRNA expressions were observed after 6 h and for the ST group, and the markers of autophagy for the CC group were increased (i.e., LC3II and Sqstm1/p62). In the heart, the Beclin 1 and ATG5 levels were downregulated for the ET group. Regarding the markers of autophagy, the Sqstm1/p62 in the heart tissue was upregulated for the END and ST groups, highlighting the beneficial effects of these exercise models. The liver protein levels of ATG5 were downregulated for the ET group. After the colchicine treatment, the liver protein levels of Sqstm1/p62 were decreased for the END and ET groups compared to the CT, ST, and CC groups. These results could be related to diabetes and obesity development or liver dysfunction improvement, demanding further investigations.

show abstract

Section: Discussionmentioning

confidence: 97%

Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice

Pinto

Rocha

Marafon

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…It has been shown that late exercise preconditioning protected the heart from exhaustive exercise-caused injuries through increasing Parkin-mediated mitophagy [114]. It was further suggested that exercise preconditioning augmented mitophagy via H 2 O 2 oxidative stress-induced activation of PI3K [115]. Consistent with this view, it was found that earlier aerobic exercise complemented by a natural herb Rhodiola sacra protected the cardiac and skeletal muscles in exhaustive exercise through enhanced mitophagy [116].…”

Section: Mitophagy and Exercisementioning

confidence: 90%

Physical Exercise and Selective Autophagy: Benefit and Risk on Cardiovascular Health

Tian

Chen

et al. 2019

Cells

View full text Add to dashboard Cite

Physical exercise promotes cardiorespiratory fitness, and is considered the mainstream of non-pharmacological therapies along with lifestyle modification for various chronic diseases, in particular cardiovascular diseases. Physical exercise may positively affect various cardiovascular risk factors including body weight, blood pressure, insulin sensitivity, lipid and glucose metabolism, heart function, endothelial function, and body fat composition. With the ever-rising prevalence of obesity and other types of metabolic diseases, as well as sedentary lifestyle, regular exercise of moderate intensity has been indicated to benefit cardiovascular health and reduce overall disease mortality. Exercise offers a wide cadre of favorable responses in the cardiovascular system such as improved dynamics of the cardiovascular system, reduced prevalence of coronary heart diseases and cardiomyopathies, enhanced cardiac reserve capacity, and autonomic regulation. Ample clinical and experimental evidence has indicated an emerging role for autophagy, a conservative catabolism process to degrade and recycle cellular organelles and nutrients, in exercise training-offered cardiovascular benefits. Regular physical exercise as a unique form of physiological stress is capable of triggering adaptation while autophagy in particular selective autophagy seems to be permissive to such cardiovascular adaptation. Here in this mini-review, we will summarize the role for autophagy in particular mitochondrial selective autophagy namely mitophagy in the benefit versus risk of physical exercise on cardiovascular function.

show abstract

“…43) Our past reports indicated that the blocked autophagy is associated with aggravated structural injury in cell and organelle. 33,34) Therefore, these results may explain how EE impairs autophagosome clearance and leads to the accumulation of LC3-II and p62, which, in turn, increases the proteotoxicity of misfolded/ damaged proteins and aggravates the myocardial ischemichypoxic injury.…”

Section: Discussionmentioning

confidence: 99%

“…These mechanisms have been previously demonstrated for their participation in EP-induced cardioprotection. 33,34) Bcl-2 is another key regulator of exercise-induced autophagy. 18) Under normal conditions, Beclin1 is inhibited by Bcl-2, whereas under stress, it dissociates from Beclin1-Bcl-2, thus, enabling the activation of PI3KC3, which induces autophagy.…”

Section: Discussionmentioning

confidence: 99%

Exercise Preconditioning Increases Beclin1 and Induces Autophagy to Promote Early Myocardial Protection via Intermittent Myocardial Ischemia-Hypoxia

et al. 2021

Self Cite

View full text Add to dashboard Cite

Exercise preconditioning (EP) provides protective effects for acute cardiovascular stress; however, its mechanisms need to be further investigated. Autophagy is a degradation pathway essential for myocardium health. Therefore, we investigated whether intermittent myocardial ischemia-hypoxia affected Beclin1 and whether the changes in autophagy levels contribute to EP-induced early myocardial protective effects. Rats were trained on a treadmill using an EP model (four cycles of 10 minutes of running/10 minutes of rest). Exhaustive exercise (EE) was performed to induce myocardial injury. Cardiac troponin I (cTnI) and ischemia-hypoxia staining were used to evaluate myocardial injury and protection. Double-labeled immunofluorescence staining and western blot analysis were employed to examine related markers. EP attenuated the myocardial ischemichypoxic injury induced by EE. Compared with the control (C) group, the dissociations of Beclin1/Bcl-2 ratio and Beclin1 expression were both higher in all other groups. Compared with the C group, PI3KC3 and the LC3-II/LC3-I ratio were higher in all other groups, whereas LC3-II was higher in the EE and EEP + EE groups. p62 was higher in the EE group than in the C group but lower in the EEP + EE group than in the EE group. We concluded that EP increases Beclin1 via intermittent myocardial ischemia-hypoxia and induces autophagy, which exerts early myocardial protective effects and reduces the myocardial ischemic-hypoxic injury induced by exhaustive exercise.

show abstract

H₂O₂ Signaling‐Triggered PI3K Mediates Mitochondrial Protection to Participate in Early Cardioprotection by Exercise Preconditioning

Cited by 22 publications

References 81 publications

Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice

Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice

Physical Exercise and Selective Autophagy: Benefit and Risk on Cardiovascular Health

Exercise Preconditioning Increases Beclin1 and Induces Autophagy to Promote Early Myocardial Protection <i>via</i> Intermittent Myocardial Ischemia-Hypoxia

Contact Info

Product

Resources

About