H19 Rises in Gastric Cancer and Exerts a Tumor-Promoting Function via miR-138/E2F2 Axis

Abstract

Purpose The aim of this paper was to investigate H19 expression in gastric cancer (GC) and its effects on the biological behavior of gastric cancer cells (GCCs), and at exploring its potential mechanism. Methods H19 expression in the patients’ tissues and serum was detected, and the correlation of the expression with the patients’ pathological data and survival rate was analyzed. Overexpression or inhibitory vectors of H19 , microRNA-138 ( miR-138 ) and E2F2 were constructed and transfected into GCCs to observe their effects on the cells’ proliferation, invasion and apoptosis. Results H19 rose in GC and was higher in GC patients with a tumor size ≥5 cm, high stages (III+IV) and lymph node metastasis. High H19 expression was associated with the poorer survival rate of the patients, so serum H19 had a certain diagnostic value for GC. H19 knockdown could inhibit GCCs to proliferate and invade and induce their apoptosis. miR-138 can be used as the target gene of H19 , and E2F2 can be negatively regulated by this miR, so miR-138 knockdown or E2F2 upregulation can weaken GCCs’ biological behavior changes that were caused by H19 knockdown. Conclusion H19 can be used as a biological indicator for diagnosing GC and predicting patients’ poor prognosis. Additionally, it promotes GCCs to proliferate and invade through miR-138 / E2F2 axis.

Abnormally Expressed lncRNAs as Potential Biomarkers for Gastric Cancer Risk: A Diagnostic Meta-Bioinformatics Analysis

“…We assessed the quality of included studies using NOS and found that the quality of the enrolled studies was acceptable. Thirty-eight studies [ 9 , 12 , 14 , 21 – 27 , 29 – 31 , 33 , 34 , 36 , 38 – 40 , 42 , 46 – 50 , 52 – 55 , 57 , 62 , 63 , 65 , 66 ] were of high quality while the other 16 studies [ 28 , 32 , 35 , 37 , 41 , 43 , 47 , 51 , 56 , 58 – 61 , 64 , 67 ] were of moderate quality.…”

“…Sample types included tissue [9,11,, circulating (plasma and serum) [12,13,47,[49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64][65], and gastric juice [21,64,66]. Most studies took specimens from Chinese population, and six studies took samples from Japanese [49],…”

Long non‐coding RNA‐H19 promotes ovarian cancer cell proliferation and migration via the microRNA‐140/Wnt1 axis

“…It is reported that H19 plays a critical role in all stages of tumorigenesis, highlighting the carcinogenic effects of H19 in a variety of cancers, such as gastric, hepatocellular, and pancreatic carcinoma. [18][19][20] Most studies have indicated that H19 is associated with growth, migration, invasion, and/or metastasis in many cancers, and serum H19 levels in patients with certain cancers have been suggested to be useful for diagnosis and prognosis. 21 Similarly, we found that H19 expression was upregulated in OC tissues and cells, and H19 overexpression was associated with poor prognosis of OC patients in this study.…”

The RNA-Binding Protein NELFE Promotes Gastric Cancer Growth and Metastasis Through E2F2

“…E2F2 expression was reported to be upregulated in gastric cancer (26,27), which is in accordance with the data of the present study. Additionally, H19 was demonstrated to promote gastric cancer cells to proliferate and invade through the miR-138/E2F2 axis (28). In cisplatin-resistant gastric cancer cells, miR-26a could improve the sensitivity of GC cells to cisplatin-based chemotherapies through E2F2 (29).…”