Haematological, biochemical and clinical signs changes following experimental infection of Streptococcus agalactiae in red hybrid tilapia (Oreochromis sp.)

Abstract

Hematological and biochemistry parameters are essential for the evaluation of fish health status; it helps provide essential information to diagnose fish diseases. This experiment was conducted to investigate the affect of Streptococcus agalactiae infection on hematological and biochemical parameters in red hybrid tilapia (Oreochromis sp.). The fish were inoculated intraperitoneally with 1 x10 4 cfu/mL bacteria then blood samples were collected and tested during the experiment. After seven days The red blood cell (RBC) counts decreased from 2.31 x 10 6 to 1.68 x 10 6. The hemoglobin (Hb) and hematocrit (PCV) counts decreased to 7.17 g/dL and 19.2% in the infected fish compared to the control group (9.71 g/dL and 28.20%). In contrast, the white blood cell (WBC) counts increased from 4.20x10 3 in the control fish to 13.2x10 3. The biochemical results initially showed an increase in glucose levels of the infected fish compared to the control from 53.82 to 101.75 on the 72 nd hour, respectively. There were no significant differences in the levels of serum total protein (T. prot), albumin (ALB), and aspartate transminase (AST) between the infected and the control fish. The inoculated fish displayed marked clinical signs such as erratic swimming behavior, exophthalmia and lethargy during the experimental. The present study has established that the changes in hematological, biochemical and clinical signs of fish can be used as an early diagnosis of pathological and physiological status in red tilapia culture. 

Comparative pathogenesis of piscirickettsiosis in Atlantic salmon (Salmo salar L.) post‐smolt experimentally challenged with LF‐89‐like and EM‐90‐like Piscirickettsia salmonis isolates

“…These results agree with reports in tilapia, Oreochromis sp. infected with Streptococcus agalactiae (Alsaid et al., ) and Edwardsiella tarda (Benli, Caḡlan, & Yavuzcan, ) and in other fish species infected with Flavobacterium columnare , A. hydrophila , Staphylococcus xylosus and Vibrio anguillarum (Maqsood, Samoon, & Singh, ; Řehulka & Minařík, ).…”

POLA INFEKSI Streptococcus agalactiae STRAIN NP105O DAN N14G PADA IKAN NILA (Oreochromis niloticus)

“…Penurunan kadar hematokrit akan memengaruhi kadar hemoglobin dan jumlah eritrosit pada ikan. Kenaikan dan penurunan kadar hematokrit dapat dijadikan indikator gejala anemia dan polisetemia pada ikan (Alsaid et al, 2014). Terjadinya anemia berdampak negatif bagi ikan karena proses metabolisme terganggu (Suhermanto et al, 2013;Hamed et al, 2019).…”

“…Rendahnya eritrosit menyebabkan terjadinya anoxia, pengaturan suhu tubuh terganggu, serta abnormalitas pertumbuhan. Hasil penelitian ikan nila merah pascainfeksi S. agalactiae menunjukkan adanya penurunan yang signifikan jumlah eritrosit, persentase hematokrit, dan hemoglobin (Alsaid et al, 2014), disebabkan organ yang memproduksi darah merah yaitu ginjal dan limpa, serta organ hemopoitik lainnya terganggu (Sirimanapong et al, 2018). Gabriel et al (2015) menyatakan peningkatan total leukosit pascainjeksi S. agalactiae merupakan respons alami ikan terhadap serangan patogen, dan dapat dijadikan sebagai indikator awal adanya infeksi patogen pada ikan.…”

“…Gabriel et al (2015) menyatakan peningkatan total leukosit pascainjeksi S. agalactiae merupakan respons alami ikan terhadap serangan patogen, dan dapat dijadikan sebagai indikator awal adanya infeksi patogen pada ikan. Pascainfeksi bakteri, ikan Jurnal Riset Akuakultur, 15 (1), 2020, 51-58 umumnya mengalami leukositosis seperti penelitian yang dilakukan pada ikan nila merah dan ikan mas (Alsaid et al, 2014;Suhermanto et al, 2013).…”

Microbial regulation and protective effects of yerba mate (Ilex paraguariensis) in gamma-irradiated mice intestine

“…The plates were incubated overnight at respective temperatures and the diameter of the clear zone was measured in millimeters. The experiment was performed in triplicates and interpretation of antibacterial properties was conducted, (Alsaid, Daud, Bejo, & Abuseliana, 2010). Inhibition zone >15 mm were categorized as strong inhibitory activity; from 10e15 mm as moderate/mild inhibitory activity; and <10 mm was low inhibitory activity.…”