Haematology for Students and Practitioners (Including Practical Haematology)

Sood, Ramnik

doi:10.5005/jp/books/10335

Cited by 3 publications

(5 citation statements)

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“…The inverse mathematical relationship in the formula, MCHC= Haemoglobin concentration X 100/PCV [20] was in agreement with the results obtained. Furthermore, normal ranges of WBC and PCV as defined by Sood [21] were also obtained in this study, to affirm the positive effect of SNS-based diets in nutrition.…”

Section: Discussionsupporting

confidence: 77%

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In-vitro lethality assay and performance of Sumain nutritional supplement on the liver enzymes and lipid profile of wistar albino rats

Emmauel

et al. 2020

GSC Biol. and Pharm. Sci.

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Sumain Nutritional Supplement (SNS) is a processed composite flour of groundnut, soya bean, guinea corn, and maize grains, and substituted at 0, 25, 50, 75, and 100% diet levels of a Commercial feed. In vitro toxicity study was conducted using Brine shrimp larvae, while in vivo performance of these diets was assessed using wistar albino rats in a 21-day feeding period. Mean body weight difference was measured. Liver extracts were assayed for alanine, and aspartate amino transaminase (ALT, AST) concentrations. Plasma extracts were assayed for haematological, and lipid profiles using standard methodologies. Results showed that SNS extract was safer than a reference tannic acid by 83%, with LC50 of 550. Compared with AST/ALT ratio of 1.17 for 100% Commercial feed, those of SNS-incorporated feeds ranged from 0.92 to 1.16, indicating no apparent damage to liver tissue. At all levels of SNS substitutions, WBC and PCV concentrations were within standard acceptable ranges, while results of MCV and Hb-related parameters exhibited very good biochemical indices of health status during the period of study. 100% and 75% SNS substituted diets particularly, showed potential to reverse thrombocytopenia. Increased Triglyceride (TG) level coupled with lowered levels of Cholesterol (LDL and TC), led to the recommendation of these two SNS diets as good nutraceuticals for human subjects having symptoms of malnutrition.

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Section: Discussionsupporting

confidence: 77%

“…On the contrary, the PLT concentration increased proportionally with SNS substitution, but were significantly lower than that of the Commercial feed alone (P<0.5). Basal PLT concentration of 150,000 had been described as normal [21].…”

Section: Discussionmentioning

confidence: 99%

In-vitro lethality assay and performance of Sumain nutritional supplement on the liver enzymes and lipid profile of wistar albino rats

Emmauel

et al. 2020

GSC Biol. and Pharm. Sci.

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“…The total erythrocyte count, TEC (Cell X 1012/L) was determined by using haemocytometer with Neuberger slide. Hayem's solution was used for this purpose as described by Sood, (1996).Total leukocyte count, TLC (Cell x 109/L).WBC was estimated by haemocytometer with Neuber improved double slide and using Thoma's SOL. The special pipte was used for WBC (Dacic and Lewis, 1984).…”

Section: Hematological Parametersmentioning

confidence: 99%

The effect of some food additive on the liver function in laboratory animal

2018

Int. J. Biosci.

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Phosphorus is an essential element as it represents the second most abundant mineral in the human body.Therefore, this study was designed to investigate the effect of phosphoric acid additive in water on some hematological and biochemical parameters in rabbits. Twelve female adult rabbits were divided randomly into two groups equally. The first group received 10% of phosphoric acid in water while the second group was received tap water as control group. The blood was collected in zero day and in 66 day. The result revealed that the Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and inorganic phosphorus were significantly increased (P <0.05) in serum of treated group as compared with control group, while the total calcium concentration was significantly decreased (P <0.05) in serum of treated group in comparison with control group. On the other hand the results of the hematological parameters showed a significant decreasing (P <0.05) in the RBC count, Hb and PCV of the treated group, whereas the WBC count was significantly (P<0.05) increased in the treated group. In conclusion, the phosphoric acid additive in water caused several changes in some biochemical and hematological parameters in rabbits.

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“…The conventional method of blood cell counting was employed [13]. A volume of 0.02 ml blood was dispensed in a test tube containing 0.38 ml of leucocyte diluent solution, and then the contents were mixed and the tube was left for three minutes.…”

Section: Total Count Of Leucocytesmentioning

confidence: 99%

“…Then the slide was stained with Leishman's stain for two minutes and buffered with Leishman's buffer for 10 minutes. After that, the slide was rinsed with tap water and left for air drying [13]. The stained smear was examined under oil immersion power (100X), and at least 200 leucocytes were randomly counted.…”

Section: Absolute Count Of Leucocytesmentioning

confidence: 99%

Immunomodulation of mice blood cells following treatment with free extract of Lactobacillus acidophilus cell

Al-Naimy¹,

Al-Ezy²,

Al-Yas³

et al. 2010

JoBRC

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A study aimed to examine immunomodulatory effect of concentrated cell free filtrate (CCF) of Lactobacillus acidophilus on immune system of mice by detecting the total and absolute counts of leucocytes. It was shown that CCF of Lactobacillus acidophilus caused a significant increase in total leucocyte count for all concentrations used in comparison with negative control. However, in absolute leucocyte counts the, CCF of L.acidophilus at doses (500 , 750) mg/kg caused a significant increased in lymphocyte, neutrophil and monocyte counts, while there was no significant differences in eosinophils and basophil cells number of mice treated with bacterial filtrate. The interaction study showed that the post-treatment with CCF of L. acidophilus caused a significant increase in total leucocytes count and absolute counts of all types of leucocyte cells except basophils and esinophils which showed no significant differences in comparison with pre-treatment

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Haematology for Students and Practitioners (Including Practical Haematology)

Cited by 3 publications

References 0 publications

In-vitro lethality assay and performance of Sumain nutritional supplement on the liver enzymes and lipid profile of wistar albino rats

In-vitro lethality assay and performance of Sumain nutritional supplement on the liver enzymes and lipid profile of wistar albino rats

The effect of some food additive on the liver function in laboratory animal

Immunomodulation of mice blood cells following treatment with free extract of Lactobacillus acidophilus cell

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