Haemocompatibility Of Non-Functionalized And Plasmachemical Functionalized Detonation Nanodiamond Particles

Abstract

The purpose of this paper is to present the innovative design of microwave plasma system for modification of detonation nanodiamond particles (DNP) using a special rotating drum placed inside the reactor. Nanodiamond particles manufactured by detonation method reveal the biological activity depending on surface functionalization. Plasmachemical modification of detonation nanodiamond particles gives the possibility of controlling surface of nanodiamonds particles in biological tests. In this paper we would like to compare detonation nanodiamond (the grain sizes from 2 to 5 nm) with modified detonation nanodiamond in rotary reactor chamber, by microwave plasma activated chemical vapour deposition (MW PACVD) method in materials research (Raman and FT-IR spectroscopy) and in vitro examinations with full of human blood. The results indicate haemocompatibility of non-modified detonation nanodiamond and modified nanodiamond by MW PACVD method in rotary reactor chamber (modified ND-3) and the presence of haemolysis in commercial detonation nanodiamond.Keywords: detonation nanodiamond particles, modified detonation nanodiamond, MW PACVD, haemocompatibilityCelem pracy jest przedstawienie innowacyjnego projektu systemu plazmy mikrofalowej z wykorzystaniem do modyfikacji detonacyjnych cząstek nanodiamontowych (DNP) przy użyciu specjalnego obrotowego bębna umieszczonego wewnątrz reaktora. Nanodiamontowe cząstki wytwarzane metodą detonacji wykazują aktywność biologiczną w zależności od funkcjonalizacji powierzchni. Plasmo-chemiczna modyfikacja detonacyjnych nanodiamontowych cząstek daje możliwość kontrolowania ich powierzchni w testach biologicznych. Autorzy w artykule porównali detonacyjny nanodiament (wielkość ziarna od 2 do 5 nm) ze zmodyfikowanym w obrotowej komorze reaktora, za pomocą procesu chemicznego osadzania wspomaganego plazmą mikrofalową (MW PACVD), detonacyjnym nanodiamentem. Wykorzystano badania materiałowe (Ramana i FT-IR spektroskopia) oraz badania in vitro na pełnej krwi ludzkiej. Badania wykazały hemozgodność niezmodyfikowanego detonacyjnego nanodiamentu i nanodiamentu zmodyfikowanego za pomocą metody MW PACVD w obrotowej komorze reaktora (zmodyfikowany ND-3) oraz obecność hemolizy w komercyjnym detonacyjnym nanodiamencie.

Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells

“…In general, NDs are characterized by low toxicity [28,29] and high biocompatibility [30]. Schrand et al [31] examined the 24 h effect of NDs on a variety of cells, including neuroblastomas, macrophages, keratinocytes, and PC-12 cells using the MTT assay and by ultrastructural cell morphology analysis.…”

The Influence of Diamond Nanoparticles on Fibroblast Cell Line L929, Cytotoxicity and Bacteriostaticity of Selected Pathogens

“…The functionalization of nanodiamond particles could be affected in two ways, i.e., doping and surface functionalization. [15][16][17].…”

Nanodiamonds for Biomedical Applications – Features of Interaction with Blood Components and Behavior in the Circulatory System [Review]

“…ND surface functionalization [49][50][51] and coating [52,53] with macromolecules by adsorption or conjugation can affect the ND in vivo safety [54,55], as well as distribution and predominant localization in organism. Thus, in the sentinel lymph nodes of both healthy mice and mice with B16 melanoma, an increased accumulation of fluorescent ND stabilized by alkynefunctionalized polyglycerol and modified by the surface with a polyvalent set of mannoses was observed [56].…”

“…Some in vitro investigations of the effect of ND effects on whole blood [49,50], certain characteristics of blood cells and their functions or other blood system components did not find any deviations [78,79], while some other properties can reveal relatively weak alterations [48,51,[76][77][78][79][80][81][82][83]. Thus, strong aggregation of detonation ND is one of the limiting factors of its bioapplications, but it has been shown that even partially disaggregated carboxylated DNA does not affect either the viability of RBC or the Raman spectra of hemoglobin (Hb) [79].…”