Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ.

Yao, Shaochang; Bai, Liang; Lan, Zuzai; Tang, Meiqiong; Zhai, Yi; Huang, Hao; Wei, Rongchang

doi:10.1007/s11240-016-0988-3

Cited by 22 publications

(15 citation statements)

References 36 publications

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“…Sucrose is the main carbon source in culture media and acts as energy and osmotic agents to support the growth and metabolism of plant cells and organs . Sucrose concentration appeared to have significant effects on the root growth and secondary metabolites production . In this study, different concentrations of sucrose (10–40 g L −1 ) in the ½ MS‐B5 medium affected the rate of hairy root growth and production of plumbagin.…”

Section: Resultsmentioning

confidence: 64%

“…19 Sucrose concentration appeared to have significant effects on the root growth and secondary metabolites production. 31,47 In this study, different concentrations of sucrose (10-40 g L −1 ) in the ½ MS-B5 medium affected the rate of hairy root growth and production of plumbagin. Biomass of transgenic roots was improved with the increased sucrose concentrations within the range of 10-30 g L −1 (Figure 9).…”

Section: Effect Of Sucrose Concentrationsmentioning

confidence: 74%

“…These features are actually significant parameters in the transformation systems in order to improve time management strategies. In addition to the bacterial strain, the type of explant is also an essential factor for effective transformation . The stem explants were significantly more suitable for infection by the strains of A. rhizogenes .…”

Section: Resultsmentioning

confidence: 99%

“…In addition to the bacterial strain, the type of explant is also an essential factor for effective transformation. 22,31 The stem explants were significantly more suitable for infection by the strains of A. rhizogenes. In comparison, hypocotyl explants showed the lowest frequency of transformation.…”

Section: Effect Of Different Strains Of a Rhizogenes And Differentmentioning

confidence: 99%

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Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Beigmohamadi

Movafeghi

Jafari

et al. 2019

Biotechnology Progress

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Plumbago europaea L. is the main source of plumbagin which is a well‐known pharmacological active compound. In this investigation, genetically transformed roots of P. europaea were obtained by improving some factors affecting the efficiency of Agrobacterium rhizoigenes‐mediated transformation such as explant type, A. rhizoigenes strain, bacterial infection period, co‐cultivation period and acetosyringone concentration. The leaf, hypocotyl and stem explants from in vitro grown plantlets were infected with bacterial strains (A4, ATCC15834, MSU440 and A13). The highest transformation rate of 69.3% was achieved after 7–9 days by inoculating A. rhizogenes MSU440 strain onto the 3‐week‐old stem explants followed by a co‐cultivation period of 2 days on a medium containing 100 μM acetosyringone. To investigate the existence of the rolB gene, polymerase chain reaction was carried out using specific primers. Effects of growth media (MS, 1/2 MS, MS‐B5 and ½ MS‐B5), different sucrose concentrations and illumination on biomass production and plumbagin biosynthesis in P. europaea hairy root cultures were analyzed using stem explants after infection with MSU440 strain. ½ MS‐B5 liquid medium containing 30 g L−1 sucrose incubated in the dark resulted in the efficient biomass production of transformed hairy roots (12.5 g fresh weight, 1.8 g dry weight) with 3.2 mg g−1 DW plumbagin accumulation. This procedure provides a framework for large‐scale cultivation of hairy roots for plumbagin production. This is the first report describing the establishment of P. europaea hairy root culture with special emphasis on plumbagin production.

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Section: Resultsmentioning

confidence: 64%

Section: Effect Of Sucrose Concentrationsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Effect Of Different Strains Of a Rhizogenes And Differentmentioning

confidence: 99%

See 2 more Smart Citations

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Beigmohamadi

Movafeghi

Jafari

et al. 2019

Biotechnology Progress

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show abstract

“…However, plant cell suspension cultures for the production of secondary metabolites have been hampered by several limitations such as low yields of desired compounds, expensive culturing process, application of phytohormones, heterogeneous cell types, lack of storage tissue and products easily degraded by the enzymes released in the media (Davies & Deroles, 2014). Hairy root cultures have been induced in many plant species which leading to the in vitro production of numerous plant secondary metabolites and pharmacologically active compounds (Bensaddek et al, 2008;Ooi et al, 2013;Nagella et al, 2013;Sharafi et al, 2014;Thwe et al, 2016;Yao et al, 2016). Genetic engineering is a modern tool which regulates the production of secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

Effect of Agrobacterium rhizogenesis on hairy roots induction in fennel (Foeniculum vulgare Miller)

Abdi¹,

Uliaie²,

Bandehagh³

et al. 2017

JEBAS

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This study was conducted to access the effects of seven Agrobacterium rhizogenes strains i.e. A4, T4, MSU, TCC15834, 2656, Gm1 and R1000, on the hairy root generation from various explants i.e. hypocotyl, cotyledon, root, stem and leaf of Fennel ecotypes (Bonab, Izmir, Esfahan and Moghan). Results of study revealed that induction of hairy root varies with the A. rhizogenes strains and used explants. Among the various tested combinations, highest root induction was recorded form hypocotyl explants using A4 and TCC 15834 (70 and 67%, respectively) strains of A. rhizogenes. Response of explant toward hairy root induction and number of the hairy roots per explant was different and decreased from hypocotyl, leaf and cotyledonary explants, respectively. Among the various tested A. rhizogenes strain, TCC 15834 capable to induced hairy Roots development in all ecotypes but most effectively in Izmir (36.66 ± 0.57) and it was followed by Bonab (32 ± 0.44), Esfahan (30 ± 0.99) and Moghan (16.66 ± 0.79). All transformed hairy root were successfully confirmed by PCR using rolB primers. Further hairy root induction could be proved as an alternative approach for the production of secondary metabolites and its increasing use as model system in metabolic engineering. KEYWORDS Fennel Agrobacterium rhizogenesHairy root culture Hairy root induction Explant type

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Hairy root culture technology: applications, constraints and prospect

Gantait

Mukherjee²

2020

Appl Microbiol Biotechnol

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Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ.

Cited by 22 publications

References 36 publications

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Potential of the genetically transformed root cultures of Plumbago europaea for biomass and plumbagin production

Effect of Agrobacterium rhizogenesis on hairy roots induction in fennel (Foeniculum vulgare Miller)

Hairy root culture technology: applications, constraints and prospect

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