Halloysite-alkaline phosphatase system—A potential bioactive component of scaffold for bone tissue engineering

Pietraszek, Aneta; Karewicz, Anna; Widnic, Marta; Lachowicz, Dorota; Gajewska, Marta; Bernasik, Andrzej; Nowakowska, Maria

doi:10.1016/j.colsurfb.2018.09.040

Cited by 36 publications

(10 citation statements)

References 22 publications

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“…This bioactive nanocomposite can also be incorporated into biomaterials used as scaffolds for tissue engineering. 53 A crucial problem that needs to be attended to in tissue engineering is forming a scaffold, which is competent enough to support the three-dimensional tissue formation The microstructures of the scaffolds could be of various types like open pore structures, fibrous matrices, hydrogels, and other natural and synthetic materials. 54 The use of HNT for tissue engineering is a relatively new venture; hence, there have been only few promising researches during the past decade.…”

Section: Gene Deliverymentioning

confidence: 99%

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Halloysite nanotubes as a nature’s boon for biomedical applications

2019

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The arena of biomedical science has long been in quest of innovative mediums for diagnostic and therapeutic applications. The latest being the use of nanomaterials for such applications, thereby giving rise to the branch of nanomedicine. Halloysite nanotubes (HNTs) are naturally occurring tubular clay nanomaterials, made of aluminosilicate kaolin sheets rolled several times. The aluminol and siloxane groups on the surface of HNT facilitate the formation of hydrogen bonding with the biomaterials onto its surface. These properties render HNT pivotal in diverse range of applications, such as in environmental sciences, waste-water treatment, dye removal, nanoelectronics and fabrication of nanocomposites, catalytic studies, as glass coatings or anticorrosive coatings, in cosmetics, as flame retardants, stimuli response, and forensic sciences. The specific properties of HNT also lead to numerous applications in biomedicine and nanomedicine, namely drug delivery, gene delivery, tissue engineering, cancer and stem cells isolation, and bioimaging. In this review, recent developments in the use of HNT for various nanomedicinal applications have been discussed.

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Section: Gene Deliverymentioning

confidence: 99%

“…This bioactive nanocomposite can also be incorporated into biomaterials used as scaffolds for tissue engineering. 53…”

Section: Applications Of Hnt In Biomedicinementioning

confidence: 99%

Halloysite nanotubes as a nature’s boon for biomedical applications

2019

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“…HNTs had a considerably more stable structure with a signi cant weight loss at 280 °C attributed to the interlayer water removal and dehydroxylation process at 415 °C (Pietraszek et al 2019). The weight loss due to the presence of TAX is not visible in the TGA curve of HNTs-TAX, which could be attributed to the low loading e ciency of TAX (Moeinpour et al 2019) and the drug con nement inside the halloysite lumen (Pietraszek et al 2019), resulting in a thermodynamically stable amorphous structure of TAX after loading process (Bavnhøj et al 2019).…”

Section: Hnts-tax Characterization Analysismentioning

confidence: 99%

Thermo-Responsive Hydrogels Based on Methylcellulose/Persian Gum Loaded With Taxifolin Enhance Bone Regeneration: An in Vitro / in Vivo Study

Sajadi-Javan

Varshosaz

Mirian

et al. 2021

Preprint

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In-situ forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25-1.5 w/v% PG and 1-7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs. In vitro release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.

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“…7 The encapsulation of alkaline phosphatase molecules in HNTs due to bone biomineralization attributable to alkaline phosphatases are considered as the main proteins in bone regeneration and remodeling which contain active immobilized enzyme and remain active in ALP-HAL NC (highest activity at pH = 13) to improve thermal stability. 64 In SEM images, chitosan/HNT films observed after immersion in simulated body fluid (SBF) can also create a dense apatite layer after days 7 and 28, thus the thickness of the apatite layer increase with the presence of HNTs. 34 Reducing the release of antibiotics in the body by controlled time-extended release via loading in HNTs and being capable of encasing drugs and molecules also allows them to be released for weeks and months, so it is one of the most promising applications for bone regeneration and a good alternative for traditional disinfection procedures.…”

Section: Bone Cement and Regenerationmentioning

confidence: 99%

Nanocontainers for drug delivery systems: A review of Halloysite nanotubes and their properties

2020

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Halloysite nanotubes (HNTs) are known as inexpensive and available nanomaterials that are rich in functionality, environmentally benign, and also safe and easy to process. As well, good particle size (i.e. nanoscale) and perfect tubular microstructures of these materials make them to be used extensively as drug carriers. Also, the unique physical and chemical properties of their internal and external surfaces are the greatest priority for the drug encapsulation controlling and releasing. In this review, is tried to emphasis on the main properties of HNTs to manage and develop effective drug delivery tools in the biomedical and pharmaceutical fields.

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Halloysite-alkaline phosphatase system—A potential bioactive component of scaffold for bone tissue engineering

Cited by 36 publications

References 22 publications

Halloysite nanotubes as a nature’s boon for biomedical applications

Halloysite nanotubes as a nature’s boon for biomedical applications

Thermo-Responsive Hydrogels Based on Methylcellulose/Persian Gum Loaded With Taxifolin Enhance Bone Regeneration: An in Vitro / in Vivo Study

Nanocontainers for drug delivery systems: A review of Halloysite nanotubes and their properties

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