Hanseniaspora terricola sp. nov., an ascomycetous yeast isolated from Tibet

Abstract

Eight apiculate strains isolated from Tibet, PR China, were identified as Hanseniaspora taiwanica and a novel species of Hanseniaspora based on the sequence analysis of the ITS region, the D1/D2 domains of the LSU rRNA and the translation elongation factor 1-a (TEF1) gene. Among them, four strains with identical sequences of D1/D2 and ITS formed a separate branch from the known Hanseniaspora species in the phylogenetic trees, and differed from the known species by at least 17 (3 %) nucleotide (nt) substitutions in the D1/D2 domains and more than 6 % substitutions and inserts/deletes in the ITS region. The phylogenetic analysis indicated that those four strains represent a novel species of Hanseniaspora, for which the names Hanseniaspora terricola sp. nov. (holotype CGMCC 2.6175T; MycoBank MB 834591) is proposed. The other four strains belonging to H. taiwanica produce spherical, void or fusiform ascospores, which differ from the original description that ascospores are absent.

Hanseniaspora menglaensis f.a., sp. nov., a novel apiculate yeast species isolated from rotting wood

“…Species of the genus Hanseniaspora have been isolated from various habitats, such as soil, flowers, fruit, tree exudates, rotten wood, bark, mushrooms, lichen, fermenting beverages and insect-associated samples [6]. They are particularly abundant on various fruits, as well as on flowers and tree barks [2, 3, 6, 7, 12, 13], which are their primary habitats [7].…”

“…[4] transferred all species of the genus Kloeckera , Kloeckera hatyaiensis , Kloeckera lindneri and Kloeckera taiwanica , to the genus Hanseniaspora on the basis of the new International Code of Nomenclature for algae, fungi, and plants . The latest incorporations to the genus were Hanseniaspora mollemarum from the Netherlands [5], Hanseniaspora gamundiae from Argentina [4], Hanseniaspora terricola from PR China [6] and Hanseniaspora smithiae from Argentina [7]. The genus currently includes 23 species, which can be separated into two lineages on the basis of phylogenetic relationships inferred using the D1/D2 domain of the large subunit (LSU) rRNA gene [5, 8–10] or from concatenation of taxonomic markers [4, 6, 7].…”

“…The latest incorporations to the genus were Hanseniaspora mollemarum from the Netherlands [5], Hanseniaspora gamundiae from Argentina [4], Hanseniaspora terricola from PR China [6] and Hanseniaspora smithiae from Argentina [7]. The genus currently includes 23 species, which can be separated into two lineages on the basis of phylogenetic relationships inferred using the D1/D2 domain of the large subunit (LSU) rRNA gene [5,[8][9][10] or from concatenation of taxonomic markers [4,6,7]. Recently, using whole-genome sequence comparison, Steenwyk et al confirmed the genus Hanseniaspora to be composed of two lineages, a fast-evolving lineage and a slow-evolving lineage, which differ in their evolutionary rates and the extent of gene loss [11].…”

“…The assembled sequences were compared with the NCBI GenBank database using the BLASTn search tool to identify similar sequences [21]. For phylogenetic placement of our isolates, we downloaded the D1/D2 domain of LSU, ITS region and ACT1 ex-type sequences of species of the genus Hanseniaspora from GenBank, following the protocol of Liu et al [6]. Wickerhamomyces anomalus CBS 5759 was selected as an outgroup taxon.…”

Hanseniaspora smithiae sp. nov., a Novel Apiculate Yeast Species From Patagonian Forests That Lacks the Typical Genomic Domestication Signatures for Fermentative Environments

“…( Čadež et al, 2019 ). The latest incorporations to the genus were Hanseniaspora mollemarum from The Netherlands ( Groenewald et al, 2018 ), Hanseniaspora gamundiae from Argentina ( Čadež et al, 2019 ), and Hanseniaspora terricola from Tibet ( Liu et al, 2021 ). Species of Hanseniaspora are widespread and particularly abundant on various fruits, as well as on flowers and bark, which are their primary habitats ( Saubin et al, 2020 ).…”