Haploids in the Improvement of Linaceae and Asteraceae

Nichterlein, K.; Horn, Renate

doi:10.1007/3-540-26889-8_14

Cited by 2 publications

(2 citation statements)

References 58 publications

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“…A number of reviews describe the production of sunflower haploid plants [ 6 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. However, the information in these reviews is very concise and rather incomplete.…”

Section: Introductionmentioning

confidence: 99%

The Production of Helianthus Haploids: A Review of Its Current Status and Future Prospects

Blinkov

Varlamova

Kurenina

et al. 2022

Plants

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The genus Helianthus comprises 52 species and 19 subspecies, with the cultivated sunflower (Helianthus annuus L.) representing one of the most important oilseed crops in the world, which is also of value for fodder and technical purposes. Currently, the leading direction in sunflower breeding is to produce highly effective heterosis F1 hybrids with increased resistance to biotic and abiotic stresses. The production of inbred parental lines via repeated self-pollination takes 4–8 years, and the creation of a commercial hybrid can take as long as 10 years. However, the use of doubled haploid technology allows for the obtainment of inbred lines in one generation, shortening the time needed for hybrid production. Moreover, it allows for the introgression of the valuable genes present in the wild Helianthus species into cultivated sunflowers. Additionally, this technology makes it possible to manipulate the ploidy level, thereby restoring fertility in interspecific hybridization. This review systematizes and analyzes the knowledge available thus far about the production of haploid and dihaploid Helianthus plants using male (isolated anther and microspore cultures) and female (unpollinated ovaries and ovules culture) gametophytes, as well as by induced parthenogenesis using γ-irradiated pollen and interspecific hybridization. The genetic, physiological, and physical factors influencing the efficiency of haploid plant production are considered. A special section focuses on the approaches used to double a haploid chromosome set and the direct and indirect methods for determining the ploidy level. The current analyzed data on the successful application of haploid sunflower plants in breeding are summarized.

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Section: Introductionmentioning

confidence: 99%

The Production of Helianthus Haploids: A Review of Its Current Status and Future Prospects

Blinkov

Varlamova

Kurenina

et al. 2022

Plants

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“…The combined use of CMS cytoplasm and a fertility restorer is a basic requirement for an efficient hybrid production in order to guarantee a 100% cross pollination and high yielding hybrids. In sunflower, commercial hybrid breeding is entirely based on the PET1 cytoplasm (Nichterlein and Horn 2005), which originated from an interspecific cross between Helianthus petiolaris and Helianthus annuus (Leclercq 1969). However, more than 70 new CMS sources have been described in sunflower (Serieys 1999).…”

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confidence: 99%

Development of markers for the use of the PEF1 cytoplasm in sunflower hybrid breeding

2008

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The use of the new cytoplasmic male sterility (CMS) source PEF1 in sunflower hybrid breeding requires markers closely linked to the restorer gene Rf_PEF1 necessary for fertility restoration of hybrids based on the PEF1 cytoplasm as well as diagnostic markers to distinguish the PEF1 cytoplasm from other cytoplasms. Bulked segregant analyses of 256 AFLP primer combinations identified 35 polymorphic primer combinations with 1-3 polymorphisms, resulting in 40 polymorphisms. Eighteen AFLP markers mapped together with the Rf_PEF1 gene covering 119.9 cM. The closest markers, E39M51_300R and E44M56_112A, mapped 3.9 and 6.0 cM to the Rf_PEF1 gene, respectively. Six SSR markers, which belong to the linkage group 13, were screened for polymorphisms between the parental lines. Only ORS630 was polymorphic, but did not map to the same linkage group as Rf_PEF1, indicating that Rf_PEF1 is not located on linkage group 13 where the restorer gene Rf1 for the PET1 cytoplasm is located. Diagnostic markers to distinguish the PEF1 cytoplasm from the PET1 and the fertile cytoplasm in sunflower were obtained using primer combinations for the atp9 gene and orfH522.

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Haploids in the Improvement of Linaceae and Asteraceae

Cited by 2 publications

References 58 publications

The Production of Helianthus Haploids: A Review of Its Current Status and Future Prospects

The Production of Helianthus Haploids: A Review of Its Current Status and Future Prospects

Development of markers for the use of the PEF1 cytoplasm in sunflower hybrid breeding

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