Haplotypes of ‘Candidatus Liberibacter solanacearum’ identified in Umbeliferous crops in Spain

Alfaro-Fernández, A.; Hernández-Llópis, D.; Font, M. I.

doi:10.1007/s10658-017-1172-2

Cited by 36 publications

(30 citation statements)

References 17 publications

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“…After the first discovery in Finland, CLso was subsequently found in symptomatic carrots in Sweden (Munyaneza et al 2012b), Norway (Munyaneza et al 2012c), Spain (Alfaro-Fernández et al 2012), France (Loiseau et al 2014), and Germany (Munyaneza et al 2015). In Spain CLso was also detected in symptomatic celery, parsnip and parsley (Alfaro-Fernández et al 2017;Teresani et al 2014). In France, CLso was recently detected in the apiaceous crops celery, chervil, fennel, parsley and parsnip, in addition to carrot, and the infected carrot, celery, parsley and chervil plants had severe symptoms (Hajri et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Carrot Pathogen ‘Candidatus Liberibacter solanacearum’ Haplotype C Detected in Symptomless Potato Plants in Finland

et al. 2018

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Candidatus Liberibacter solanacearum' (CLso) haplotype C, a bacterial pathogen transmitted by the carrot psyllid Trioza apicalis, causes yield losses in carrot production. Due to concerns that this pathogen might also threaten potato (Solanum tuberosum) production, the occurrence of CLso in cultivated and volunteer potatoes in Tavastia Proper and Satakunta regions of Finland was studied. Volunteer potato plants were found in 13 of the 27 inspected carrot fields. Of the 148 potato samples tested by PCR, eight volunteer potato plants and one cultivated potato grown at the edge of a carrot field were found CLso positive. The PCR products obtained from these potatoes with primers OA2/OI2c, LpFrag4-1611F/LpFrag4-480R and CL514F/CL514R all showed 100% sequence identity to CLso haplotype C. This is the first observation of CLso haplotype C in field-grown potatoes. In addition, transmission experiments were performed. Attempts to transmit CLso into potato with carrot psyllids were not successful; however, CLso haplotype C was transmitted from infected carrots to potato plants by leaf grafting and by phloem connection formed by dodder, a parasitic plant, and found to survive in the potato plants for several weeks after transmission. However, the bacterial colonization progressed slowly in the potato phloem, and the amount of bacteria detected was low. The plants produced from the daughter tubers of the CLso positive potato plants were all CLso negative, suggesting that CLso haplotype C was not able to pass to the daughter plants. None of the CLso positive potatoes inoculated in greenhouse or collected from fields showed symptoms characteristic of zebra chip disease, associated with CLso haplotypes A and B.

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Section: Introductionmentioning

confidence: 99%

Carrot Pathogen ‘Candidatus Liberibacter solanacearum’ Haplotype C Detected in Symptomless Potato Plants in Finland

et al. 2018

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“…For Lso, few studies have been attempted to investigate its population structure and genetic diversity, in particular due to its fastidious nature (Lin and Gudmestad, 2013). Sequencing of the 16S rRNA, 16S/23S ISR and 50S rpIJ - rpIL ribosomal gene loci has become the method of choice for the study of the genetic diversity of the bacterium (Lin et al, 2009; Wen et al, 2009; Nelson et al, 2011, 2013; Teresani et al, 2014; Alfaro-Fernández et al, 2017; Haapalainen et al, 2017; Hajri et al, 2017; Monger and Jeffries, 2018). However, genetic variation within these genes does not generally allow differentiation of closely related strains due to their high degree of conservation (Lin and Gudmestad, 2013).…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Genetic Diversity of the Bacterial Plant Pathogen ‘Candidatus Liberibacter solanacearum’ as Revealed by a New Multilocus Sequence Analysis Scheme

Hajri¹,

Cousseau-Suhard²,

Gentit³

et al. 2019

Preprint

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10'Candidatus Liberibacter solanacearum' (Lso) has emerged as a serious threat on solanaceous 11 and apiaceous crops worldwide. Five Lso haplotypes (LsoA, LsoB, LsoC, LsoD and LsoE) 12 have been identified so far. To decipher genetic relationships between Lso strains, a MLSA 13 study of seven housekeeping genes (acnA, atpD, ftsZ, glnA, glyA, gnd and groEL) was 14 performed on a representative bacterial collection of 49 Lso strains. In all, 5415 bp spanning 15 the seven loci were obtained from each of the 49 strains of our bacterial collection. Analysis of 16 sequence data was consistent with a clonal population structure with no evidence of 17 recombination. Phylogenies reconstructed from individual genes, and with concatenated data, 18 were globally congruent with each other. In addition to the five highly supported and distinct 19 genetic clusters, which correspond to the five established haplotypes, our phylogenetic data 20 revealed the presence of a sixth haplotype, designated 'LsoG'. This new haplotype is currently 21 represented by two strains from France which had distinct sequences in four out of the seven 22 tested housekeeping genes. Altogether, the data presented here provide new information 23 regarding the genetic structure of Lso and the evolutionary history of the haplotypes defined 24 within this bacterial species.25 26

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“…The modern medicine uses parsnip for treating and preventing cardiovascular diseases. The medical norm of consuming parsnip is 10 kg for a person per year [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Study of the Influence of the Ripeness Degree of Parsnip Roots and Storage Method on Their Preservation

Pusik

Lyubymova

et al. 2019

EUREKA: Life Sciences

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Sowing parsnip is recommended to be harvested in the stage of technical ripeness that takes place in 120–150 days after sprouts. A ripeness degree cannot be determined by a root size, because in this case planting conditions and agrotechnique play an important role. The aim of the research was to analyze the influence of a vegetation period duration of different sorts of sowing parsnip on formation of chemical composition components and roots preservation to determine the dependence between the content of dry soluble substances and one of dry substances, sugars, saccharose, reducing sugars, ascorbic acid. The research gives a possibility to get roots with the maximal content of chemical composition components and long storage period. There has been studied the influence of a vegetation period duration of different sorts of sowing parsnip on formation of chemical composition components and roots preservation. The dependence between the content of dry soluble substances and one of dry substances, sugars, saccharose, reducing sugars, ascorbic acid has been determined. The research has been conducted with parsnip roots of sowing sort Petrik, Student, Boris. There has been studied the influence of a ripeness degree (vegetation period duration) on a quality and preservation of parsnip roots. Parsnip roots of the sowing sorts Student and Boris are characterized by the most content of the dry substance 27,1–25,4 %, and content of the total sugar 5,7–5,4 %, high content of vitamin С 10,2–10,1 mg/100 g. It has been established, that the studied sorts are not inclined to nitrates accumulation, their content varies from 60 to 80 mg/kg and is lower than the maximally permitted level (MPL 250 mg/kg). There has been established the direct strong connection between the content of the dry substance and sugars content (r=0,98), nitrates content (r=0,80) and the middle content with the testing mark (r=–0,54) and vitamin С (r=0,57). The dry soluble substance content has the direct strong connection with the sugars content (r=0,99), nitrates content (r=0,78) and middle connection with the content of vitamin С (r=0,46) and reverse middle connection with the testing mark (r=–0,52). The reverse middle connection exists between the sugars content and the testing mark (r=–0,52) and the direct strong connection with nitrates content (r=0,84), middle connection with the content of vitamin C (r=0,48). At storage of roots the loss of the mass of dry substances is from 11,5 % at storage in an open box to 6,2 – in a polyethylene film. The most changes take place in saccharose and monosaccharides, 36,6 and 33,0 % respectively. The content of vitamin C decreases by 30 % at storage in an open box and by 10 % in a polyethylene film. The vegetation period duration as 150 days is characterized by the lower sugars content, but at that the ratio saccharose: monosacharides – the most. There has been offered the method of determining the ripeness degree of parsnip roots by the saccharose content. At the technical ripeness the amount of saccharose is 72–75 % of the total amount of sugars. The optimal ripeness is characterized by deceleration of growing processes, decrease of saccharose accumulation rates, the most ratio of the roots mass to the one of leaves and saccharose to monosugars. The method of roots storage with p. e. film decreases losses of nutrients of roots and increases the storage duration.

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Haplotypes of ‘Candidatus Liberibacter solanacearum’ identified in Umbeliferous crops in Spain

Cited by 36 publications

References 17 publications

Carrot Pathogen ‘Candidatus Liberibacter solanacearum’ Haplotype C Detected in Symptomless Potato Plants in Finland

Carrot Pathogen ‘Candidatus Liberibacter solanacearum’ Haplotype C Detected in Symptomless Potato Plants in Finland

New Insights into the Genetic Diversity of the Bacterial Plant Pathogen ‘Candidatus Liberibacter solanacearum’ as Revealed by a New Multilocus Sequence Analysis Scheme

Study of the Influence of the Ripeness Degree of Parsnip Roots and Storage Method on Their Preservation

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