HB-EGF expression as a potential biomarker of acquired middle ear cholesteatoma

Xie, Shuang; Wang, Xiaoli; Ren, Hongmiao; Liu, Xiaoyu; Ren, Jun; Liu, Wei

doi:10.1080/00016489.2017.1284343

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…Although many studies have investigated the mechanisms of cholesteatoma formation, its pathogenesis remains unclear [1]. Many molecules and proteins have been investigated [13][14][15][16]. Studies on cholesteatoma tissue have shown that epidermal growth factor receptor (EGFR) disorder plays a role in the development of cholesteatoma.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of periostin, fibronectin and tenascin-C in patients chronic otitis media with cholesteatoma

Birinci

Terzi

Demir

et al. 2022

Egypt J Otolaryngol

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Background Although many studies have investigated the mechanisms of cholesteatoma formation, its pathogenesis remains unclear. The aim of this study was to evaluate the levels of periostin, fibronectin and tenascin-C, which are involved in many mechanisms, such as cell adhesion, cell differentiation, inflammation, fibrosis, angiogenesis and cell proliferation, in patients with chronic otitis media (COM). A total of 80 participants, 65 COM patients undergoing surgery and 15 healthcare personnel volunteers serving as controls, were included in this study. The participants were divided into four groups: cholesteatoma, granulation, avivation and control group. The serum periostin, fibronectin and tenascin-C levels of all participants were determined biochemically. Histopathological evaluation of tissue samples and 20 skin samples used as controls was performed by immunohistochemistry. Results Of the 65 patients, 22 presented with cholesteatoma, 15 with granulation tissue and 28 with the edge of the tympanic membrane perforation freshening tissue. There were no significant differences in serum periostin, fibronectin and tenascin-C levels between the groups. In the immunohistochemical evaluation, fibronectin and periostin staining was significantly more intense in the cholesteatoma group than in the other groups (p = 0.001). Epithelial tenascin-C staining was significantly more intense in the avivation group than in the other groups (p = 0.041). Conclusion The levels of periostin and fibronectin were higher in cholesteatoma tissue than in other forms of chronic otitis and skin tissue. This suggests that they may be involved in the mechanism of cholesteatoma formation. These proteins could be used as biomarkers in the diagnosis and treatment of cholesteatoma.

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Section: Discussionmentioning

confidence: 99%

Evaluation of periostin, fibronectin and tenascin-C in patients chronic otitis media with cholesteatoma

Birinci

Terzi

Demir

et al. 2022

Egypt J Otolaryngol

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“…Cholesteatoma is known as a severe chronic middle ear disease that occurs in the middle ear, eventually causing adverse complications with rapid growth and bone resorption [ 23 ]. It accounts for 0.5–1.8% of all brain tumors [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma

Yao

Zhang

Zheng

et al. 2021

BioMed Research International

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Introduction. miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear. Methods. Detection by qRT-PCR was conducted on miR-199a’s expression in both thirty pairs of cholesteatoma tissues and normal skins. For characterizing the function of miR-199a, this research adopted transwell assay, wound healing assay, and CCK8 assays. Under the support of qRT-PCR, efforts were made to investigate the relative expression of candidate target genes. Moreover, the evaluation of the targeting relationship between miR-199a and the candidate target gene was conducted with the dual-luciferase reporter assay. Results. The upregulation of miR-199a was found in cholesteatoma tissues, which facilitated the proliferation, migration, and invasion of HaCaT cells, while its downregulation caused opposite results. Conclusions. The findings of the present research offer more insights into the molecular mechanism of cholesteatoma progression.

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“…In the last decade it has become obvious that there is not one gene suitable for use of normalisation for all tissue types [ 40 ]. In the field of middle ear research many different genes have been used, such as B2M [ 52 ], PPIA [ 9 ], ACTB [ 33 , 34 ], HPRT1 [ 35 ] and GAPDH [ 36 – 39 ]. Interestingly, all articles published to this date use only one gene for normalisation, even though it has been abundantly shown that this is not sufficient for reliable analysis [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…QPCR has been used in several studies related to diseases of the middle ear. However, there seems to be little consensus as to which reference genes to use for normalisation, leading to the use of several different genes without proper establishment of which genes are appropriate [9,[31][32][33][34][35][36][37][38][39]. To be able to obtain reliable results the use of stably expressed reference genes for normalisation is essential [40].…”

Section: Introductionmentioning

confidence: 99%

Extensive qPCR analysis reveals altered gene expression in middle ear mucosa from cholesteatoma patients

Drakskog¹,

Klerk²,

Westerberg³

et al. 2020

PLoS ONE

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The middle ear is a small and hard to reach compartment, limiting the amount of tissue that can be extracted and the possibilities for studying the molecular mechanisms behind diseases like cholesteatoma. In this paper 14 reference gene candidates were evaluated in the middle ear mucosa of cholesteatoma patients and two different control tissues. ACTB and GAPDH were shown to be the optimal genes for the normalisation of target gene expression when investigating middle ear mucosa in multiplex qPCR analysis. Validation of reference genes using c-MYC expression confirmed the suitability of ACTB and GAPDH as reference genes and showed an upregulation of c-MYC in middle ear mucosa during cholesteatoma. The occurrence of participants of the innate immunity, TLR2 and TLR4, were analysed in order to compare healthy middle ear mucosa to cholesteatoma. Analysis of TLR2 and TLR4 showed variable results depending on control tissue used, highlighting the importance of selecting relevant control tissue when investigating causes for disease. It is our belief that a consensus regarding reference genes and control tissue will contribute to the comparability and reproducibility of studies within the field.

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HB-EGF expression as a potential biomarker of acquired middle ear cholesteatoma

Cited by 7 publications

References 17 publications

Evaluation of periostin, fibronectin and tenascin-C in patients chronic otitis media with cholesteatoma

Evaluation of periostin, fibronectin and tenascin-C in patients chronic otitis media with cholesteatoma

miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma

Extensive qPCR analysis reveals altered gene expression in middle ear mucosa from cholesteatoma patients

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